PMID- 30931468
OWN - NLM
STAT- MEDLINE
DCOM- 20190828
LR  - 20190828
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 19
IP  - 9
DP  - 2019 Apr 23
TI  - Immature dendritic cells navigate microscopic mazes to find tumor cells.
PG  - 1665-1675
LID - 10.1039/c9lc00150f [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells with high sentinel 
      ability to scan their neighborhood and to initiate an adaptive immune response. 
      Whereas chemotactic migration of mature DCs (mDCs) towards lymph nodes is 
      relatively well documented, the migratory behavior of immature DCs (imDCs) in 
      tumor microenvironments is still poorly understood. Here, microfluidic systems of 
      various geometries, including mazes, are used to investigate how the physical and 
      chemical microenvironment influences the migration pattern of imDCs. Under proper 
      degree of confinement, the imDCs are preferentially recruited towards cancer vs. 
      normal cells, accompanied by increased cell speed and persistence. Furthermore, a 
      systematic screen of cytokines, reveals that Gas6 is a major chemokine 
      responsible for the chemotactic preference. These results and the accompanying 
      theoretical model suggest that imDC migration in complex tissue environments is 
      tuned by a proper balance between the strength of the chemical gradients and the 
      degree of spatial confinement.
FAU - Um, Eujin
AU  - Um E
AD  - Center for Soft and Living Matter, Institute for Basic Science (IBS), Ulsan, 
      44919, Republic of Korea.
FAU - Oh, Jung Min
AU  - Oh JM
FAU - Park, Juhee
AU  - Park J
FAU - Song, Taegeun
AU  - Song T
FAU - Kim, Tae-Eon
AU  - Kim TE
FAU - Choi, Yongjun
AU  - Choi Y
FAU - Shin, Changsik
AU  - Shin C
FAU - Kolygina, Diana
AU  - Kolygina D
FAU - Jeon, Jae-Hyung
AU  - Jeon JH
FAU - Grzybowski, Bartosz A
AU  - Grzybowski BA
FAU - Cho, Yoon-Kyoung
AU  - Cho YK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
RN  - 0 (Cytokines)
SB  - IM
EIN - Lab Chip. 2019 Sep 10;19(18):3140. PMID: 31468052
MH  - Animals
MH  - Cell Line
MH  - *Cell Movement
MH  - Chemotaxis
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
EDAT- 2019/04/02 06:00
MHDA- 2019/08/29 06:00
CRDT- 2019/04/02 06:00
PHST- 2019/04/02 06:00 [pubmed]
PHST- 2019/08/29 06:00 [medline]
PHST- 2019/04/02 06:00 [entrez]
AID - 10.1039/c9lc00150f [doi]
PST - ppublish
SO  - Lab Chip. 2019 Apr 23;19(9):1665-1675. doi: 10.1039/c9lc00150f.