PMID- 30932727
OWN - NLM
STAT- MEDLINE
DCOM- 20201014
LR  - 20201014
IS  - 1439-7609 (Electronic)
IS  - 1439-7595 (Linking)
VI  - 30
IP  - 3
DP  - 2020 May
TI  - Pharmaceutical immunoglobulins reduce neutrophil extracellular trap formation and 
      ameliorate the development of MPO-ANCA-associated vasculitis.
PG  - 544-550
LID - 10.1080/14397595.2019.1602292 [doi]
AB  - Objectives: Intravenous immunoglobulin (IVIG) therapy is effective against some 
      autoimmune diseases. We examined the effects of pharmaceutical immunoglobulins on 
      the development of MPO-ANCA-associated vasculitis (MPO-AAV).Methods: Peripheral 
      blood neutrophils were pretreated with 5 mg/ml sulfo-immunoglobulins (IVIG-S) and 
      then exposed to 100 nM phorbol myristate acetate (PMA). Thereafter, neutrophil 
      extracellular traps (NETs) were detected by flow cytometry. Next, Wistar-Kyoto 
      rats were given oral administration of 10 mg/kg/day propylthiouracil for 28 days 
      and intraperitoneal (i.p.) injection of 1 mug PMA on days 0 and 7. These rats were 
      divided into two groups: Group 1 with i.p. injection of 400 mg/kg IVIG-S on days 
      8-12 and Group 2 with vehicle similarly. ANCA titers were chronologically 
      determined by indirect immunofluorescence. On day 28, all rats were killed to 
      examine NET formation in the peritoneum and the development of AAV.Results: 
      IVIG-S significantly inhibited NET formation induced by PMA in vitro. NET amounts 
      in the peritoneum in Group 1 were significantly smaller than in Group 2, and ANCA 
      titers in Group 1 were significantly lower than in Group 2. The degree of 
      pulmonary hemorrhage in Group 1 was also smaller than in Group 2.Conclusion: 
      IVIG-S reduce NET formation and ameliorate the development of MPO-AAV.
FAU - Uozumi, Ryo
AU  - Uozumi R
AD  - Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido 
      University, Sapporo, Japan.
AD  - Division of Laboratory and Transfusion Medicine, Hokkaido University Hospital, 
      Sapporo, Japan.
FAU - Iguchi, Risa
AU  - Iguchi R
AD  - Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido 
      University, Sapporo, Japan.
FAU - Masuda, Sakiko
AU  - Masuda S
AD  - Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido 
      University, Sapporo, Japan.
FAU - Nishibata, Yuka
AU  - Nishibata Y
AD  - Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido 
      University, Sapporo, Japan.
FAU - Nakazawa, Daigo
AU  - Nakazawa D
AD  - Division of Rheumatology, Endocrinology and Nephrology, Faculty of Medicine and 
      Graduate School of Medicine, Hokkaido University, Sapporo, Japan.
FAU - Tomaru, Utano
AU  - Tomaru U
AD  - Department of Pathology, Faculty of Medicine and Graduate School of Medicine, 
      Hokkaido University, Sapporo, Japan.
FAU - Ishizu, Akihiro
AU  - Ishizu A
AD  - Department of Medical Laboratory Science, Faculty of Health Sciences, Hokkaido 
      University, Sapporo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20190412
PL  - England
TA  - Mod Rheumatol
JT  - Modern rheumatology
JID - 100959226
RN  - 0 (Immunoglobulins, Intravenous)
SB  - IM
MH  - Animals
MH  - Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis/*drug 
      therapy/metabolism
MH  - Extracellular Traps/*metabolism
MH  - Immunoglobulins, Intravenous/pharmacology/*therapeutic use
MH  - Male
MH  - Neutrophils/*drug effects/metabolism
MH  - Rats
OTO - NOTNLM
OT  - ANCA-associated vasculitis
OT  - Anti-neutrophil cytoplasmic antibody (ANCA)
OT  - animal model
OT  - intravenous immunoglobulin (IVIG)
OT  - neutrophil extracellular traps (NETs)
EDAT- 2019/04/02 06:00
MHDA- 2020/10/21 06:00
CRDT- 2019/04/02 06:00
PHST- 2019/04/02 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/04/02 06:00 [entrez]
AID - 10.1080/14397595.2019.1602292 [doi]
PST - ppublish
SO  - Mod Rheumatol. 2020 May;30(3):544-550. doi: 10.1080/14397595.2019.1602292. Epub 
      2019 Apr 12.