PMID- 30935947
OWN - NLM
STAT- MEDLINE
DCOM- 20200528
LR  - 20200528
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 379
IP  - 1
DP  - 2019 Jun 1
TI  - Identification of stemness in primary retinoblastoma cells by analysis of 
      stem-cell phenotypes and tumorigenicity with culture and xenograft models.
PG  - 110-118
LID - S0014-4827(19)30134-X [pii]
LID - 10.1016/j.yexcr.2019.03.034 [doi]
AB  - Retinoblastoma (RB) is a primary intraocular malignancy in childhood, and may 
      develop relapse and metastatic disease. This study was to identify the stem-cell 
      properties of primary retinoblastoma cells critical to tumorigenesis and 
      metastasis. Primary cells were isolated from fresh human RB tissues after 
      enucleation, and cultured in serum-free or serum-enriched conditions, with two RB 
      cell-lines Weri-RB1 and Y79 for comparison. Proliferation of primary RB cells 
      were well-maintained in serum-free condition of DMEM/F12 medium, and formed 
      stem-cell like spheroids. The immaturity of cultured primary RB cells was 
      demonstrated by tendency of highly expressed stem-cell markers (CD133, Nestin and 
      OCT4) and suppressed mature retinal-cell markers (GFAP, MAP2 and Recoverin). 
      CD133, a neural stem-cell marker being exclusively studied in RB, was found 
      positive in small patches of cells in archival human RB by immunohistochemistry. 
      Meanwhile, at initial isolation, insignificant CD133(+) cells were detected by 
      flow-cytometry, and substantial increase of positivity was observed after several 
      days cultivated in serum-free condition. Cultured primary RB cells were engrafted 
      in subretinal region of BALB/c nude mice for assessment of tumorigenicity. Strong 
      tumorigenic activity and extensive progression of the xenograft retinoblastoma 
      was induced by primary cells as compared with the two cell-lines. Again, 
      immunohistochemistry confirmed that the stem-cell markers were emphasized in the 
      xenograft tumor in mice. Our findings demonstrated that in comparison to the 
      well-established RB cell-lines, cultured primary RB cells possess stem-cell like 
      properties with highly expressed stem-cell markers, self-regenerative growth in 
      culture, and strong in vivo oncogenic potentiality.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Tang, Zhixin
AU  - Tang Z
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Ma, Huan
AU  - Ma H
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Mao, Yuxiang
AU  - Mao Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Ai, Siming
AU  - Ai S
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Zhang, Ping
AU  - Zhang P
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Nie, Cong
AU  - Nie C
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Gao, Yang
AU  - Gao Y
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China.
FAU - Lu, Rong
AU  - Lu R
AD  - State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen 
      University, Guangzhou, China. Electronic address: rongluzz@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190330
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (AC133 Antigen)
RN  - 0 (Biomarkers)
SB  - IM
MH  - AC133 Antigen/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Carcinogenesis/metabolism/pathology
MH  - Cell Line, Tumor
MH  - Child
MH  - Disease Models, Animal
MH  - Heterografts
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Neoplastic Stem Cells/metabolism/*pathology
MH  - Neural Stem Cells/metabolism/*pathology
MH  - Phenotype
MH  - Retinal Neoplasms/metabolism/*pathology
MH  - Retinoblastoma/metabolism/*pathology
OTO - NOTNLM
OT  - Primary cells
OT  - Retinoblastoma
OT  - Stem-cell characteristics
OT  - Tumorigenesis
OT  - Xenograft
EDAT- 2019/04/03 06:00
MHDA- 2020/05/29 06:00
CRDT- 2019/04/03 06:00
PHST- 2019/01/23 00:00 [received]
PHST- 2019/03/14 00:00 [revised]
PHST- 2019/03/27 00:00 [accepted]
PHST- 2019/04/03 06:00 [pubmed]
PHST- 2020/05/29 06:00 [medline]
PHST- 2019/04/03 06:00 [entrez]
AID - S0014-4827(19)30134-X [pii]
AID - 10.1016/j.yexcr.2019.03.034 [doi]
PST - ppublish
SO  - Exp Cell Res. 2019 Jun 1;379(1):110-118. doi: 10.1016/j.yexcr.2019.03.034. Epub 
      2019 Mar 30.