PMID- 30936600
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220408
IS  - 0253-0716 (Print)
IS  - 1735-3688 (Electronic)
IS  - 0253-0716 (Linking)
VI  - 44
IP  - 2
DP  - 2019 Mar
TI  - Bone Marrow Stromal Cells with the Granulocyte Colony-Stimulating Factor in the 
      Management of Chemotherapy-Induced Ovarian Failure in a Rat Model.
PG  - 135-145
AB  - BACKGROUND: Bone marrow stromal cells (BMSCs), as a type of mesenchymal stem 
      cells, and the granulocyte colony-stimulating factor (G-CSF), as a type of growth 
      factor, may recover damaged ovaries. The aim of the present study was to 
      investigate the effects of the coadministration of BMSCs and the G-CSF on damaged 
      ovaries after creating a chemotherapy model with cyclophosphamide (CTX) in rats. 
      METHODS: The present study was performed in Semnan, Iran, in the late 2016 and 
      the early 2017. BMSCs were cultured and were confirmed using the CD markers of 
      stromal cells. Forty female Wistar rats were randomly divided into 4 groups. The 
      rats were injected intraperitoneally with CTX for 14 days to induce chemotherapy 
      and ovarian destruction. Then, the BMSCs were injected into bilateral ovaries and 
      the G-CSF was injected intraperitoneally, individually and together. Four weeks 
      later, the number of ovarian follicles using H&E staining, the number of 
      apoptotic granulosa cells using the TUNEL assay, the number of produced oocytes 
      from the ovaries, and the levels of serum E2 and FSH using an ELISA reader were 
      assessed. Statistical analysis was done using one-way ANOVA with SPSS, version 
      16.0. RESULTS: The results showed that the effects of the coadministration of 
      2x10(6) BMSCs and 70 microg/kg of the G-CSF were significantly more favorable than 
      those in the control group (P<0.001), the BMSC group (P=0.016), and the G-CSF 
      group (P<0.001) on the recovery of damaged ovaries. CONCLUSION: The efficacy of 
      the coadministration of BMSCs and the G-CSF in the recovery of ovaries damaged by 
      chemotherapy was high by comparison with the administration of either of them 
      separately.
FAU - Sameni, Hamid Reza
AU  - Sameni HR
AD  - Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University 
      of Medical Sciences, Semnan, Iran.
FAU - Seiri, Maryam
AU  - Seiri M
AD  - Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University 
      of Medical Sciences, Semnan, Iran.
FAU - Safari, Manouchehr
AU  - Safari M
AD  - Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University 
      of Medical Sciences, Semnan, Iran.
FAU - Tabrizi Amjad, Mohammad Hasan
AU  - Tabrizi Amjad MH
AD  - Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University 
      of Medical Sciences, Semnan, Iran.
FAU - Khanmohammadi, Nasrin
AU  - Khanmohammadi N
AD  - Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University 
      of Medical Sciences, Semnan, Iran.
FAU - Zarbakhsh, Sam
AU  - Zarbakhsh S
AD  - Nervous System Stem Cells Research Center, Faculty of Medicine, Semnan University 
      of Medical Sciences, Semnan, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Med Sci
JT  - Iranian journal of medical sciences
JID - 8104374
PMC - PMC6423433
OTO - NOTNLM
OT  - Chemotherapy
OT  - Granulocyte colony-stimulating factor
OT  - Ovary
OT  - Regeneration
OT  - Mesenchymal stromal cells
EDAT- 2019/04/03 06:00
MHDA- 2019/04/03 06:01
PMCR- 2019/03/01
CRDT- 2019/04/03 06:00
PHST- 2019/04/03 06:00 [entrez]
PHST- 2019/04/03 06:00 [pubmed]
PHST- 2019/04/03 06:01 [medline]
PHST- 2019/03/01 00:00 [pmc-release]
AID - IJMS-44-2 [pii]
PST - ppublish
SO  - Iran J Med Sci. 2019 Mar;44(2):135-145.