PMID- 30938456
OWN - NLM
STAT- MEDLINE
DCOM- 20200622
LR  - 20200622
IS  - 1527-3350 (Electronic)
IS  - 0270-9139 (Linking)
VI  - 70
IP  - 1
DP  - 2019 Jul
TI  - Characteristics of Impaired Dendritic Cell Function in Patients With Hepatitis B 
      Virus Infection.
PG  - 25-39
LID - 10.1002/hep.30637 [doi]
AB  - Dendritic cells (DCs) are antigen-presenting cells with a central role in host 
      immune response. This study analyzed gene expression and DC function in hepatitis 
      B virus (HBV) patients, functions impaired because of HBV, and identified the 
      genes related to these functions. Peripheral blood mononuclear cells from 64 HBV 
      patients and 19 healthy controls were analyzed. Peripheral blood DCs were stained 
      with antibodies against human leukocyte antigen-DR/Lin-1/CD123/CD11c and 
      separated into plasmacytoid DCs (pDCs) and myeloid DCs by fluorescence-activated 
      cell sorting. Using an interferon-gamma enzyme-linked immunospot assay, we 
      analyzed antigen-specific response in HBV-infected patients. Regarding DC 
      function, we analyzed antigen-presenting capacity, cell migration capacity, 
      phagocytic capacity, and cytokine production capacity. DC gene expression was 
      analyzed by microarray to identify genes related to DC function. No difference 
      was found in the number of DCs in peripheral blood between healthy participants 
      and HBV patients. In cell-surface marker analysis, CD80, CD83, CD86, CD40, and 
      C-C motif chemokine receptor 7 expression levels in pDCs were related to the 
      HBV-specific T-cell response. DCs from HBV patients exhibited decreases in 
      antigen-presenting capacity, migration capacity, and cytokine production 
      capacity. In gene expression analysis, immune-related genes with greatly reduced 
      expression levels in chronic hepatitis B patients were identified. Of these 
      genes, interleukin (IL)-6 signal transducer (IL6ST) expression level positively 
      correlated with DC surface marker expression level. Adjustment of IL6ST 
      expression level in DCs and treatment with oncostatin M resulted in recovery of 
      DC function. Conclusion: IL6ST expression was identified as one cause of decline 
      in DC function in HBV patients. Adjustment of IL6 family cytokine signaling may 
      be useful for recovering reduced DC function in HBV infection.
CI  - (c) 2019 by the American Association for the Study of Liver Diseases.
FAU - Yonejima, Atsushi
AU  - Yonejima A
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Mizukoshi, Eishiro
AU  - Mizukoshi E
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Tamai, Toshikatsu
AU  - Tamai T
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Nakagawa, Hidetoshi
AU  - Nakagawa H
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Kitahara, Masaaki
AU  - Kitahara M
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Yamashita, Tatsuya
AU  - Yamashita T
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Arai, Kuniaki
AU  - Arai K
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Terashima, Takeshi
AU  - Terashima T
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Iida, Noriho
AU  - Iida N
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Fushimi, Kazumi
AU  - Fushimi K
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Okada, Hikari
AU  - Okada H
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Yamashita, Taro
AU  - Yamashita T
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Sakai, Yoshio
AU  - Sakai Y
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Honda, Masao
AU  - Honda M
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
FAU - Kaneko, Shuichi
AU  - Kaneko S
AD  - Department of Gastroenterology, Graduate School of Medicine, Kanazawa University, 
      Kanazawa City, Japan.
LA  - eng
GR  - JP16fk0310508/AMED/International
GR  - JP19fk0310107/AMED/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190524
PL  - United States
TA  - Hepatology
JT  - Hepatology (Baltimore, Md.)
JID - 8302946
SB  - IM
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Dendritic Cells/*metabolism
MH  - Female
MH  - Gene Expression Profiling
MH  - Hepatitis B/*immunology
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - T-Lymphocytes, Cytotoxic/physiology
EDAT- 2019/04/03 06:00
MHDA- 2020/06/23 06:00
CRDT- 2019/04/03 06:00
PHST- 2018/10/23 00:00 [received]
PHST- 2019/03/25 00:00 [accepted]
PHST- 2019/04/03 06:00 [pubmed]
PHST- 2020/06/23 06:00 [medline]
PHST- 2019/04/03 06:00 [entrez]
AID - 10.1002/hep.30637 [doi]
PST - ppublish
SO  - Hepatology. 2019 Jul;70(1):25-39. doi: 10.1002/hep.30637. Epub 2019 May 24.