PMID- 30939790
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20200225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 7
DP  - 2019 Apr 1
TI  - Quantitative Determination of Acetamiprid in Pollen Based on a Sensitive 
      Enzyme-Linked Immunosorbent Assay.
LID - 10.3390/molecules24071265 [doi]
LID - 1265
AB  - A sensitive biotinylated indirect competitive enzyme-linked immunosorbent assay 
      (Bic-ELISA) was developed to detect acetamiprid pesticides in pollen, based on 
      the heterogeneous coating antigen and biotinylated anti-acetamiprid monoclonal 
      antibody. Under optimized experimental conditions, the detection limit for the 
      Bic-ELISA was 0.17 ng/mL and the linear range was 0.25(-)25 ng/mL. The 
      cross-reactivities could be regarded as negligible for the biotinylated 
      antibodies with their analogues except for thiacloprid (1.66%). Analyte 
      recoveries for extracts of spiked pollen (camellia pollen, lotus pollen, rape 
      pollen) ranged from 81.1% to 108.0%, with intra-day relative standard deviations 
      (RSDs) of 4.8% to 10.9%, and the average reproducibility was 85.4% to 110.9% with 
      inter-assay and inter-assay RSDs of 6.1% to 11.7%. The results of Bic-ELISA 
      methods for the Taobao's website samples were largely consistent with HPLC-MS/MS. 
      Therefore, the established Bic-ELISA methods would be conducive to the monitoring 
      of acetamiprid in pollen.
FAU - Fang, Qingkui
AU  - Fang Q
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      qkfang@163.com.
AD  - Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests 
      of Anhui Higher Education Institutes, Anhui Agricultural University, Hefei 
      230036, China. qkfang@163.com.
FAU - Zu, Quan
AU  - Zu Q
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      zuquan06053122@163.com.
AD  - Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests 
      of Anhui Higher Education Institutes, Anhui Agricultural University, Hefei 
      230036, China. zuquan06053122@163.com.
FAU - Hua, Xiude
AU  - Hua X
AD  - College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, 
      China. huaxiude@njau.edu.cn.
FAU - Lv, Pei
AU  - Lv P
AD  - School of Resource & Environment, Anhui Agricultural University, Hefei 230036, 
      China. lvpei@ahau.edu.cn.
FAU - Lin, Wanwen
AU  - Lin W
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      mei.mei906888037@163.com.
FAU - Zhou, Dahe
AU  - Zhou D
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      17352900322@163.com.
FAU - Xu, Zihan
AU  - Xu Z
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      zhmirror03@sina.com.
FAU - Fan, Jiarui
AU  - Fan J
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      15156879285@163.com.
FAU - Li, Xiaohan
AU  - Li X
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      lixianhan99@sina.com.
FAU - Cao, Haiqun
AU  - Cao H
AUID- ORCID: 0000-0003-3653-3450
AD  - School of Plant Protection, Anhui Agricultural University, Hefei 230036, China. 
      caohaiqun@ahau.edu.cn.
AD  - Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests 
      of Anhui Higher Education Institutes, Anhui Agricultural University, Hefei 
      230036, China. caohaiqun@ahau.edu.cn.
LA  - eng
GR  - 31701808/National Natural Science Foundation of China/
GR  - GJFP2019011/Agro-product Quality Safety Risk Assessment of the Ministry of 
      Agriculture of the People's Republic of China/
PT  - Journal Article
DEP - 20190401
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Neonicotinoids)
RN  - 5HL5N372P0 (acetamiprid)
MH  - Antibodies, Monoclonal/*immunology
MH  - Camellia/*chemistry
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Lotus/*chemistry
MH  - Neonicotinoids/*analysis/immunology
MH  - Pollen/*chemistry
PMC - PMC6479566
OTO - NOTNLM
OT  - Bic-ELISA
OT  - acetamiprid
OT  - biotinylated mAb
OT  - heterogeneous coating antigen
OT  - pesticide
OT  - pollen
COIS- The authors declare no conflict of interest.
EDAT- 2019/04/04 06:00
MHDA- 2019/08/03 06:00
PMCR- 2019/04/01
CRDT- 2019/04/04 06:00
PHST- 2019/02/27 00:00 [received]
PHST- 2019/03/27 00:00 [revised]
PHST- 2019/03/30 00:00 [accepted]
PHST- 2019/04/04 06:00 [entrez]
PHST- 2019/04/04 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
PHST- 2019/04/01 00:00 [pmc-release]
AID - molecules24071265 [pii]
AID - molecules-24-01265 [pii]
AID - 10.3390/molecules24071265 [doi]
PST - epublish
SO  - Molecules. 2019 Apr 1;24(7):1265. doi: 10.3390/molecules24071265.