PMID- 30940568
OWN - NLM
STAT- MEDLINE
DCOM- 20200113
LR  - 20231213
IS  - 1872-7573 (Electronic)
IS  - 0378-8741 (Linking)
VI  - 238
DP  - 2019 Jun 28
TI  - Natural dietary antioxidants containing flavonoids modulate keratinocytes 
      physiology: In vitro tri-culture models.
PG  - 111844
LID - S0378-8741(19)30190-4 [pii]
LID - 10.1016/j.jep.2019.111844 [doi]
AB  - ETHNOPHARMACOLOGICAL RELEVANCE: Several traditional medicinal herbs are widely 
      used for dermatologic and cosmetic preparations. The beneficial skin repair 
      activity is detected in various phases of wound-healing process, such as 
      cell-cell, cell-matrix interactions or collagen synthesis. AIM OF THE STUDY: The 
      study assessed the effects of Opuntia ficus-indica (L.) Mill. (Opuntia) and Milk 
      Thistle (MT) (Silybum marianum (L.) Gaerth) on adult keratinocytes (HaCaT) 
      functioning under basal condition or in the presence of mechanical damage 
      (wounded cells). MATERIALS AND METHODS: The role of the natural compounds was 
      tested on HaCaT grown in mono-culture and tri-culture configurations. In 
      tri-cultures models, HaCaT were treated with the conditioned media (CM) obtained 
      by Human Normal Dermal Fibroblast (NHDF) and Human Dermal Microvascular 
      Endothelial cells (HMVEC) co-cultures. Specifically, were tested cell viability, 
      oxidative stress mechanisms (cytokines release and lipid peroxidation) and 
      cellular remodelling (growth factors release or metalloproteinase modulation). 
      Moreover, the migratory potential of HaCaT was analysed by the use of wound 
      healing in vitro assay. RESULTS: Opuntia and MT differently modified the 
      metabolism (EGF, MMP-9), and the migratory properties of HaCaT both under 
      physiological conditions or upon mechanical damage (wounded cells). Moreover, 
      both compounds modulated HaCaT response to oxidative stress. The response to the 
      natural compounds were modified, and in some cases potentiated, in tri-culture 
      configuration systems. CONCLUSIONS: The data demonstrated that in vitro 
      tri-culture approach is suitable to characterize the role of natural compounds on 
      the complex communication between dermal-epidermal cellular components and 
      microvascular endothelium. Specifically, Opuntia and MT are good alternatives to 
      synthetic compounds in skin repair promotion.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Bassino, Eleonora
AU  - Bassino E
AD  - Department of Life Sciences and Systems Biology, University of Torino, Italy. 
      Electronic address: eleonora.bassino@unito.it.
FAU - Gasparri, Franco
AU  - Gasparri F
AD  - Department of Pharmacy, University of Salerno, Italy.
FAU - Munaron, Luca
AU  - Munaron L
AD  - Department of Life Sciences and Systems Biology, University of Torino, Italy.
LA  - eng
PT  - Journal Article
DEP - 20190330
PL  - Ireland
TA  - J Ethnopharmacol
JT  - Journal of ethnopharmacology
JID - 7903310
RN  - 0 (Antioxidants)
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Flavonoids)
SB  - IM
MH  - Antioxidants/*pharmacology
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Cell Survival/drug effects
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Diet
MH  - Endothelial Cells/*drug effects/physiology
MH  - Fibroblasts/*drug effects/physiology
MH  - Flavonoids/*pharmacology
MH  - Humans
MH  - Keratinocytes/*drug effects/physiology
MH  - *Silybum marianum
MH  - *Opuntia
MH  - Wound Healing/drug effects
OTO - NOTNLM
OT  - Endothelial cells
OT  - Keratinocytes
OT  - Milk thistle
OT  - Opuntia ficus-Indica (L.) Mill.
OT  - Wound-healing
EDAT- 2019/04/04 06:00
MHDA- 2020/01/14 06:00
CRDT- 2019/04/04 06:00
PHST- 2019/01/14 00:00 [received]
PHST- 2019/03/21 00:00 [revised]
PHST- 2019/03/27 00:00 [accepted]
PHST- 2019/04/04 06:00 [pubmed]
PHST- 2020/01/14 06:00 [medline]
PHST- 2019/04/04 06:00 [entrez]
AID - S0378-8741(19)30190-4 [pii]
AID - 10.1016/j.jep.2019.111844 [doi]
PST - ppublish
SO  - J Ethnopharmacol. 2019 Jun 28;238:111844. doi: 10.1016/j.jep.2019.111844. Epub 
      2019 Mar 30.