PMID- 30941001
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1662-4548 (Print)
IS  - 1662-453X (Electronic)
IS  - 1662-453X (Linking)
VI  - 13
DP  - 2019
TI  - Cx43-Associated Secretome and Interactome Reveal Synergistic Mechanisms for 
      Glioma Migration and MMP3 Activation.
PG  - 143
LID - 10.3389/fnins.2019.00143 [doi]
LID - 143
AB  - Extracellular matrix (ECM) remodeling, degradation and glioma cell motility are 
      critical aspects of glioblastoma multiforme (GBM). Despite being a rich source of 
      potential biomarkers and targets for therapeutic advance, the dynamic changes 
      occurring within the extracellular environment that are specific to GBM motility 
      have yet to be fully resolved. The gap junction protein connexin43 (Cx43) 
      increases glioma migration and invasion in a variety of in vitro and in vivo 
      models. In this study, the upregulation of Cx43 in C6 glioma cells induced 
      morphological changes and the secretion of proteins associated with cell 
      motility. Demonstrating the selective engagement of ECM remodeling networks, 
      secretome analysis revealed the near-binary increase of osteopontin and matrix 
      metalloproteinase-3 (MMP3), with gelatinase and NFF-3 assays confirming the 
      proteolytic activities. Informatic analysis of interactome and secretome 
      downstream of Cx43 identifies networks of glioma motility that appear to be 
      synergistically engaged. The data presented here implicate ECM remodeling and 
      matrikine signals downstream of Cx43/MMP3/osteopontin and ARK1B10 inhibition as 
      possible avenues to inhibit GBM.
FAU - Aftab, Qurratulain
AU  - Aftab Q
AD  - Department of Cellular and Physiological Sciences, Life Sciences Institute, 
      University of British Columbia, Vancouver, BC, Canada.
FAU - Mesnil, Marc
AU  - Mesnil M
AD  - Signalisation et Transports Ioniques Membranaires (STIM), CNRS ERL 7003, 
      University of Poitiers, Poitiers, France.
FAU - Ojefua, Emmanuel
AU  - Ojefua E
AD  - Department of Chemistry, Brandon University, Brandon, MB, Canada.
FAU - Poole, Alisha
AU  - Poole A
AD  - Department of Chemistry, Brandon University, Brandon, MB, Canada.
FAU - Noordenbos, Jenna
AU  - Noordenbos J
AD  - Department of Chemistry, Brandon University, Brandon, MB, Canada.
FAU - Strale, Pierre-Olivier
AU  - Strale PO
AD  - Department of Cellular and Physiological Sciences, Life Sciences Institute, 
      University of British Columbia, Vancouver, BC, Canada.
FAU - Sitko, Chris
AU  - Sitko C
AD  - Department of Chemistry, Brandon University, Brandon, MB, Canada.
FAU - Le, Caitlin
AU  - Le C
AD  - Department of Chemistry, Brandon University, Brandon, MB, Canada.
FAU - Stoynov, Nikolay
AU  - Stoynov N
AD  - Department of Biochemistry and Molecular Biology, Centre for High-Throughput 
      Biology, University of British Columbia, Vancouver, BC, Canada.
FAU - Foster, Leonard J
AU  - Foster LJ
AD  - Department of Biochemistry and Molecular Biology, Centre for High-Throughput 
      Biology, University of British Columbia, Vancouver, BC, Canada.
FAU - Sin, Wun-Chey
AU  - Sin WC
AD  - Signalisation et Transports Ioniques Membranaires (STIM), CNRS ERL 7003, 
      University of Poitiers, Poitiers, France.
FAU - Naus, Christian C
AU  - Naus CC
AD  - Signalisation et Transports Ioniques Membranaires (STIM), CNRS ERL 7003, 
      University of Poitiers, Poitiers, France.
FAU - Chen, Vincent C
AU  - Chen VC
AD  - Department of Chemistry, Brandon University, Brandon, MB, Canada.
LA  - eng
PT  - Journal Article
DEP - 20190319
PL  - Switzerland
TA  - Front Neurosci
JT  - Frontiers in neuroscience
JID - 101478481
PMC - PMC6433981
OTO - NOTNLM
OT  - ARK1B10
OT  - MMP3
OT  - connexin43
OT  - gap junction
OT  - glioma
OT  - migration
OT  - osteopontin
OT  - secretome
EDAT- 2019/04/04 06:00
MHDA- 2019/04/04 06:01
PMCR- 2019/01/01
CRDT- 2019/04/04 06:00
PHST- 2017/09/27 00:00 [received]
PHST- 2019/02/07 00:00 [accepted]
PHST- 2019/04/04 06:00 [entrez]
PHST- 2019/04/04 06:00 [pubmed]
PHST- 2019/04/04 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fnins.2019.00143 [doi]
PST - epublish
SO  - Front Neurosci. 2019 Mar 19;13:143. doi: 10.3389/fnins.2019.00143. eCollection 
      2019.