PMID- 30941822 OWN - NLM STAT- MEDLINE DCOM- 20190529 LR - 20190529 IS - 1520-6327 (Electronic) IS - 0739-4462 (Linking) VI - 101 IP - 2 DP - 2019 Jun TI - Molecular cloning, expression profiling, and functional analysis of a broad-complex isoform 2/3 (Br-Z2/Z3) transcription factor in the diamondback moth, Plutella xylostella (L.). PG - e21549 LID - 10.1002/arch.21549 [doi] AB - The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a widespread and destructive pest of cruciferous crops. New strategies for controlling it are needed because it is rapidly developing resistance to conventional pesticides. In insects, transcription factors (TFs) including broad-complex (Br-C) are thought to be useful for insecticide development because they are able to regulate the transcription of functional genes involved in responses to external stimuli including insecticides. In the present study, we cloned and sequenced the open reading frames (ORFs) of three BTB-ZF encoding genes from the diamondback moth deposited in the National Center for Biotechnology Information (NCBI) database under accessions MG753773, MG288674, and MG753772. The lengths of these ORFs were 1,680, 1,428, and 1,647 bp, respectively. The phylogenetic analysis based on the predicted amino acid sequences of ZF domains showed that MG753773 and MG288674 belonged to Z2/Z3 and Z7 of Br-C while MG753772 belonged to Ttk types. In the agreement, the highest expression level of MG753773 occurred during the prepupal stage, MG288674 and MG753772 were expressed during all stages and peaked in the adult and egg stages, respectively. RNA interference silencing of MG753773 in the late third instar larvae significantly decreased survival and pupation of the insects. With precocene II, transcription of MG753773 increased (4x) in the fourth instar larva 24 hr later; 48 hr later the rate of prepupation and pupation was significantly higher. These findings will contribute to the development of new regulators of the growth and development for diamondback moth control. CI - (c) 2019 Wiley Periodicals, Inc. FAU - Huang, Jingfei AU - Huang J AD - College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China. FAU - Fang, Ling AU - Fang L AD - College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China. FAU - Wang, Shuai AU - Wang S AD - College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China. FAU - Liu, Xiang AU - Liu X AD - College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China. FAU - Chen, Yong AU - Chen Y AD - Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. AD - Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. FAU - Chen, Yixin AU - Chen Y AD - Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. AD - Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. FAU - Tian, Houjun AU - Tian H AD - Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. AD - Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. FAU - Lin, Shuo AU - Lin S AD - Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. AD - Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. FAU - Tian, Sufen AU - Tian S AD - College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China. FAU - Wei, Hui AU - Wei H AD - Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. AD - Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian Province, China. FAU - Gu, Xiaojun AU - Gu X AUID- ORCID: 0000-0003-1504-0577 AD - College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China. LA - eng GR - 31240090/National Natural Science Foundation of China/ GR - 31701806/National Natural Science Foundation of China/ GR - 2014J01087/Natural Science Foundation of Fujian Province/ GR - KFA17140A and KFA 17382A/Development Foundation of Science and Technology of Fujian Agriculture and Forestry University/ GR - KF2015062/Development Foundation of Science and Technology of Fujian Agriculture and Forestry University/ PT - Journal Article DEP - 20190403 PL - United States TA - Arch Insect Biochem Physiol JT - Archives of insect biochemistry and physiology JID - 8501752 RN - 0 (Insect Proteins) RN - 0 (Protein Isoforms) RN - 0 (Transcription Factors) SB - IM MH - Amino Acid Sequence MH - Animals MH - Cloning, Molecular MH - Gene Expression Regulation, Developmental MH - Insect Proteins/genetics/*metabolism MH - Life Cycle Stages/genetics MH - Moths/genetics/*metabolism MH - Open Reading Frames MH - Phylogeny MH - Protein Isoforms/genetics/metabolism MH - RNA Interference MH - Transcription Factors/genetics/*metabolism OTO - NOTNLM OT - Plutella xylostella OT - RNA interference OT - broad-complex isoform 2/3 OT - expression profiling OT - metamorphosis OT - open reading frame OT - precocene II EDAT- 2019/04/04 06:00 MHDA- 2019/05/30 06:00 CRDT- 2019/04/04 06:00 PHST- 2019/01/25 00:00 [received] PHST- 2019/02/23 00:00 [revised] PHST- 2019/03/06 00:00 [accepted] PHST- 2019/04/04 06:00 [pubmed] PHST- 2019/05/30 06:00 [medline] PHST- 2019/04/04 06:00 [entrez] AID - 10.1002/arch.21549 [doi] PST - ppublish SO - Arch Insect Biochem Physiol. 2019 Jun;101(2):e21549. doi: 10.1002/arch.21549. Epub 2019 Apr 3.