PMID- 30944642
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 17
IP  - 5
DP  - 2019 May
TI  - Label-free microfluidic chip for the identification of mesothelial cell clusters 
      in pleural effusion.
PG  - 4532-4544
LID - 10.3892/ol.2019.10118 [doi]
AB  - The detection of tumor cells and clusters in pleural effusion assists in the 
      diagnosis of lung cancer. The proportion of tumor cells and clusters to the total 
      number of cells in each patient varies substantially due to individual 
      differences and the severity of the disease. The identification of one tumor cell 
      or cluster from a large number of pleural effusions is the main challenge for 
      hydrothorax tumor cell detection techniques. In the present study, by using A549 
      lung cancer and Met-5A mesothelial cell lines, a label-free microfluidic chip 
      based on cell cluster size was designed. By setting the parameters of the chip, 
      individual cells and clusters were able to enter different microfluidic channels. 
      Subsequent to non-specific staining, the recovered components were stained using 
      acridine orange (AO). A charge-coupled device camera was used to captured images 
      of the cell, and the features of these cells were analyzed in their R and G 
      channels using Matlab software to establish the characteristics and finally 
      differentiate between the tumor and non-tumor cell or clusters. According to the 
      results, when inlet A and B were under a velocity of 10 and 8.5 ml/h, 
      respectively, the tumor cell clusters were successfully collected through 
      microfluidic channels III-V, with a recovery rate of ~80%. Subsequent to staining 
      with AO, the feature values in the R and G channels were identified, and initial 
      differentiation was achieved. The present study combined the microfluidic chip, 
      which is based on cluster size, with a computer identification method for pleural 
      effusion. The successful differentiation of tumor cell clusters from non-tumor 
      clusters provides the basis for the identification of tumor clusters in 
      hydrothorax.
FAU - Zhao, Lili
AU  - Zhao L
AD  - Department of Laboratory Science, School of Laboratory Medicine, Tianjin Medical 
      University, Tianjin 300203, P.R. China.
FAU - Zhao, Meng
AU  - Zhao M
AD  - Key Laboratory of Computer Vision and System of Ministry of Education, School of 
      Computer Science and Engineering, Tianjin University of Technology, Tianjin 
      300384, P.R. China.
FAU - Yang, Yu
AU  - Yang Y
AD  - Department of Laboratory Science, School of Laboratory Medicine, Tianjin Medical 
      University, Tianjin 300203, P.R. China.
FAU - Gu, Yajun
AU  - Gu Y
AD  - Department of Laboratory Science, School of Laboratory Medicine, Tianjin Medical 
      University, Tianjin 300203, P.R. China.
FAU - Zheng, Fang
AU  - Zheng F
AD  - Department of Laboratory Science, School of Laboratory Medicine, Tianjin Medical 
      University, Tianjin 300203, P.R. China.
FAU - Wang, Xuan
AU  - Wang X
AD  - Department of Clinical Laboratory, Tianjin Chest Hospital, Tianjin 300051, P.R. 
      China.
FAU - Zheng, Zhiyuan
AU  - Zheng Z
AD  - Department of Bone Science, North China University of Science and Technology 
      Affiliated Hospital, Tangshan, Hebei 063000, P.R. China.
FAU - Sun, Xuguo
AU  - Sun X
AD  - Department of Laboratory Science, School of Laboratory Medicine, Tianjin Medical 
      University, Tianjin 300203, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190306
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC6444474
OTO - NOTNLM
OT  - acridine orange
OT  - cell cluster
OT  - image processing
OT  - lung cancer
OT  - mesothelial cell
OT  - microfluidic chip
OT  - pleural effusion
EDAT- 2019/04/05 06:00
MHDA- 2019/04/05 06:01
PMCR- 2019/03/06
CRDT- 2019/04/05 06:00
PHST- 2018/02/02 00:00 [received]
PHST- 2019/01/28 00:00 [accepted]
PHST- 2019/04/05 06:00 [entrez]
PHST- 2019/04/05 06:00 [pubmed]
PHST- 2019/04/05 06:01 [medline]
PHST- 2019/03/06 00:00 [pmc-release]
AID - OL-0-0-10118 [pii]
AID - 10.3892/ol.2019.10118 [doi]
PST - ppublish
SO  - Oncol Lett. 2019 May;17(5):4532-4544. doi: 10.3892/ol.2019.10118. Epub 2019 Mar 
      6.