PMID- 30944807
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231006
IS  - 2190-572X (Print)
IS  - 2190-5738 (Electronic)
IS  - 2190-5738 (Linking)
VI  - 9
IP  - 4
DP  - 2019 Apr
TI  - Molecular characterization of laccase genes from the basidiomycete Trametes 
      hirsuta Bm-2 and analysis of the 5' untranslated region (5'UTR).
PG  - 160
LID - 10.1007/s13205-019-1691-y [doi]
LID - 160
AB  - The aim of this study was to identify and characterize laccase genes produced by 
      Trametes hirsuta Bm-2 in a liquid medium, both with and without induction. The 
      amplification of 5'and 3'regions of laccase sequences was obtained by the 
      RACE-PCR method, and these were assembled to obtain a cDNA of total length. Two 
      new laccase genes were isolated from basal medium (lac-B) and lignocellulosic 
      grapefruit substrate (lac-T), both encoding open reading frames of 2566 bp. Both 
      laccase-predicted proteins consisted of 521 amino acids, four copper-binding 
      regions, a signal peptide, and five potential glycosilation sites 
      (Asn-Xaa-Ser/Tre). Moreover, the deduced amino acid sequences share about 76-85% 
      identity with other laccases of WRF. Sequence comparison showed 47 synonymous 
      point mutations between lac-B and lac-T. In addition, 5' untranslated regions 
      (UTR) of laccase genes lac-B and lac-T showed differences in length and number of 
      regulatory elements that may affect transcriptional or translational expression 
      of these genes.
FAU - Pereira-Patron, Alejandrina
AU  - Pereira-Patron A
AD  - 1Depto. de Ingenieria Quimica y Bioquimica, Tecnologico Nacional de Mexico, 
      Instituto Tecnologico de Merida, Av. Tecnologico Km 4.5 S/N, 97118 Merida, 
      Yucatan Mexico. ISNI: 0000 0000 8806 6098. GRID: grid.462454.5
FAU - Solis-Pereira, Sara
AU  - Solis-Pereira S
AD  - 1Depto. de Ingenieria Quimica y Bioquimica, Tecnologico Nacional de Mexico, 
      Instituto Tecnologico de Merida, Av. Tecnologico Km 4.5 S/N, 97118 Merida, 
      Yucatan Mexico. ISNI: 0000 0000 8806 6098. GRID: grid.462454.5
FAU - Lizama-Uc, Gabriel
AU  - Lizama-Uc G
AD  - 1Depto. de Ingenieria Quimica y Bioquimica, Tecnologico Nacional de Mexico, 
      Instituto Tecnologico de Merida, Av. Tecnologico Km 4.5 S/N, 97118 Merida, 
      Yucatan Mexico. ISNI: 0000 0000 8806 6098. GRID: grid.462454.5
FAU - Ramirez-Prado, Jorge H
AU  - Ramirez-Prado JH
AD  - 2Unidad de Biotecnologia, Centro de Investigacion Cientifica de Yucatan, Calle 43 
      No. 130 x 32 y 34, Col. Chuburna de Hidalgo, 97205 Merida, Yucatan Mexico. ISNI: 
      0000 0004 0428 7635. GRID: grid.418270.8
FAU - Perez-Brito, Daisy
AU  - Perez-Brito D
AUID- ORCID: 0000-0003-2116-0065
AD  - 3Laboratorio GeMBio, Centro de Investigacion Cientifica de Yucatan, Calle 43 No. 
      130 x 32 y 34, Col. Chuburna de Hidalgo, 97205 Merida, Yucatan Mexico. ISNI: 0000 
      0004 0428 7635. GRID: grid.418270.8
FAU - Tapia-Tussell, Raul
AU  - Tapia-Tussell R
AUID- ORCID: 0000-0001-5124-6156
AD  - 4Unidad de Energia Renovable, Centro de Investigacion Cientifica de Yucatan, 
      Carretera Sierra Papacal-Chuburna Puerto Km 5, 97302 Merida, Yucatan Mexico. 
      ISNI: 0000 0004 0428 7635. GRID: grid.418270.8
LA  - eng
PT  - Journal Article
DEP - 20190330
PL  - Germany
TA  - 3 Biotech
JT  - 3 Biotech
JID - 101565857
PMC - PMC6441420
OTO - NOTNLM
OT  - Laccase genes
OT  - RACE-PCR
OT  - Trametes hirsuta
OT  - Untranslated regions
COIS- Compliance with ethical standardsThe authors declare that there is no conflict of 
      interest regarding publication of this paper.
EDAT- 2019/04/05 06:00
MHDA- 2019/04/05 06:01
PMCR- 2020/04/01
CRDT- 2019/04/05 06:00
PHST- 2018/12/07 00:00 [received]
PHST- 2019/03/21 00:00 [accepted]
PHST- 2019/04/05 06:00 [entrez]
PHST- 2019/04/05 06:00 [pubmed]
PHST- 2019/04/05 06:01 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - 1691 [pii]
AID - 10.1007/s13205-019-1691-y [doi]
PST - ppublish
SO  - 3 Biotech. 2019 Apr;9(4):160. doi: 10.1007/s13205-019-1691-y. Epub 2019 Mar 30.