PMID- 30945184
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20190802
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1933
DP  - 2019
TI  - Subcellular Localization and Functions of Plant lncRNAs in Drought and Salt 
      Stress Tolerance.
PG  - 173-186
LID - 10.1007/978-1-4939-9045-0_9 [doi]
AB  - Plant lncRNAs are expected to play important roles in various plant processes 
      including response to abiotic stress, although the functions of most plant 
      lncRNAs are still unknown. To investigate these potential functions, integrated 
      approaches that employ genetic, transgenic, and molecular biology methods are 
      required. Here, we describe general methods to study the function of lncRNAs in 
      plant response to drought and salt stresses. First, the expression pattern and 
      subcellular localization of lncRNA are analyzed by GUS staining and RNA 
      fluorescence in situ hybridization (FISH). Then the responses of lncRNA mutant 
      and overexpressing transgenic plants to drought and salt stress treatments are 
      characterized, and their sensitivities to ABA are also assayed. To understand the 
      molecular mechanism of lncRNAs' function in stress response, transcriptome 
      sequencing (RNA-seq) and real-time quantitative PCR are performed to analyze 
      altered expression of stress-related genes. Finally, proline content and ROS 
      content are measured to reveal the accumulation of osmolytes and second 
      messengers in these plants in response to drought and salt stress treatments.
FAU - Qin, Tao
AU  - Qin T
AD  - Texas A&M AgriLife Research Center, Dallas, TX, USA.
FAU - Xiong, Liming
AU  - Xiong L
AD  - Texas A&M AgriLife Research Center, Dallas, TX, USA. lxiong@hkbu.edu.hk.
AD  - Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong. 
      lxiong@hkbu.edu.hk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Plant)
MH  - Arabidopsis/*genetics
MH  - Arabidopsis Proteins/*genetics
MH  - DNA Methylation
MH  - Gene Expression Regulation, Plant
MH  - RNA, Long Noncoding/*genetics
MH  - RNA, Plant/*genetics
MH  - *Salt Tolerance
MH  - *Stress, Physiological
MH  - Subcellular Fractions/*metabolism
OTO - NOTNLM
OT  - Drought stress
OT  - FISH
OT  - GUS staining
OT  - Plant lncRNAs
OT  - Proline content
OT  - ROS content
OT  - Salt stress
OT  - Stress response genes
OT  - Transcriptome sequencing
EDAT- 2019/04/05 06:00
MHDA- 2019/08/03 06:00
CRDT- 2019/04/05 06:00
PHST- 2019/04/05 06:00 [entrez]
PHST- 2019/04/05 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
AID - 10.1007/978-1-4939-9045-0_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1933:173-186. doi: 10.1007/978-1-4939-9045-0_9.