PMID- 30946611
OWN - NLM
STAT- MEDLINE
DCOM- 20200501
LR  - 20200501
IS  - 1502-7708 (Electronic)
IS  - 0036-5521 (Linking)
VI  - 54
IP  - 4
DP  - 2019 Apr
TI  - Roseburia intestinalis inhibits oncostatin M and maintains tight junction 
      integrity in a murine model of acute experimental colitis.
PG  - 432-440
LID - 10.1080/00365521.2019.1595708 [doi]
AB  - Objective: Levels of oncostatin M (OSM) and the composition of gut microbiota 
      predict responses to anti-TNF agents used for IBD therapy. Here, the aim was to 
      investigate the effects of Roseburia intestinalis, a gut microbiota, on OSM and 
      on intestinal barrier in colitis. Methods: In the murine model of 3% dextran 
      sulfate sodium (DSS)-induced colitis, we tested disease activity index (DAI), 
      colon length, histological score and expression of tight junction (TJ) proteins 
      (ZO-1, occludin and claudin-1), OSM, TNF-alpha and TLR5. In addition, a cellular 
      model was used to examine the role of R. intestinalis during secretion of OSM by 
      lipopolysaccharide (LPS)-induced bone marrow-derived macrophages (BMDMs) isolated 
      from wild-type (WT) and TLR5 knockout (TLR5 KO) mice. Furthermore, we evaluated 
      the impact of OSM on expressions of TJ proteins by Caco-2 cells. Results: R. 
      intestinalis in DSS-induced colitis decreased DAI score (p < .001), colon length 
      shortening (6.46 +/- 0.36 cm vs 5.65 +/- 0.47 cm, p = .022), histological score 
      (2.667 +/- 1.15 vs 5.33 +/- 1.14, p = .018) and increased expression of TJ proteins 
      (p < .05). In addition, R. intestinalis reduced expression of OSM (p < .05) and 
      TNF-alpha (p < .05), while increasing expression of TLR5 (p < .05). Furthermore, R. 
      intestinalis reduced secretion of OSM (p < .05) by LPS-induced BMDMs isolated 
      from WT and TLR5 KO mice. Moreover, OSM downregulated expression of TJ proteins 
      (p < .05) by Caco-2 cells in a concentration-dependent manner. Conclusions: These 
      results indicate that R. intestinalis attenuates inflammation in IBD by 
      decreasing secretion of OSM and by promoting intestinal barrier function. Taken 
      together, the data provide insight into the role of the gut microbiota in 
      patients with IBD who are resistant to anti-TNF therapy.
FAU - Tan, Bei
AU  - Tan B
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Luo, Weiwei
AU  - Luo W
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Shen, Zhaohua
AU  - Shen Z
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Xiao, Mengwei
AU  - Xiao M
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Wu, Shuai
AU  - Wu S
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Meng, Xiangrui
AU  - Meng X
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Wu, Xing
AU  - Wu X
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Yang, Zhenyu
AU  - Yang Z
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Tian, Li
AU  - Tian L
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
FAU - Wang, Xiaoyan
AU  - Wang X
AD  - a Department of Gastroenterology , Third Xiangya Hospital, Central South 
      University , Changsha , China.
AD  - b Hunan Key Laboratory of Nonresolving Inflammation and Cancer , Changsha , 
      China.
LA  - eng
PT  - Journal Article
DEP - 20190404
PL  - England
TA  - Scand J Gastroenterol
JT  - Scandinavian journal of gastroenterology
JID - 0060105
RN  - 0 (Tlr5 protein, mouse)
RN  - 0 (Toll-Like Receptor 5)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 106956-32-5 (Oncostatin M)
RN  - 9042-14-2 (Dextran Sulfate)
RN  - Roseburia intestinalis
SB  - IM
MH  - Animals
MH  - Caco-2 Cells
MH  - Clostridiales/*physiology
MH  - Colitis/*metabolism/microbiology
MH  - Colon/pathology
MH  - Dextran Sulfate
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Intestinal Mucosa/microbiology/*pathology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Oncostatin M/*metabolism
MH  - Tight Junctions/*metabolism
MH  - Toll-Like Receptor 5/genetics
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Microbiota
OT  - anti-TNF therapy
OT  - colitis
OT  - oncostatin M
OT  - tight junction
EDAT- 2019/04/05 06:00
MHDA- 2020/05/02 06:00
CRDT- 2019/04/05 06:00
PHST- 2019/04/05 06:00 [pubmed]
PHST- 2020/05/02 06:00 [medline]
PHST- 2019/04/05 06:00 [entrez]
AID - 10.1080/00365521.2019.1595708 [doi]
PST - ppublish
SO  - Scand J Gastroenterol. 2019 Apr;54(4):432-440. doi: 
      10.1080/00365521.2019.1595708. Epub 2019 Apr 4.