PMID- 30946940
OWN - NLM
STAT- MEDLINE
DCOM- 20200214
LR  - 20200214
IS  - 1878-4216 (Electronic)
IS  - 0278-5846 (Linking)
VI  - 93
DP  - 2019 Jul 13
TI  - Early intervention with electroacupuncture prevents PTSD-like behaviors in rats 
      through enhancing hippocampal endocannabinoid signaling.
PG  - 171-181
LID - S0278-5846(19)30005-3 [pii]
LID - 10.1016/j.pnpbp.2019.03.018 [doi]
AB  - Electroacupuncture (EA) is a clinically useful physiological therapy that has 
      been recently adopted to treat several brain disorders. However, the potential 
      role of early EA intervention in the prevention of posttraumatic stress disorder 
      (PTSD) as well as its potential cellular and molecular mechanism has never been 
      investigated previously. In the present study, we used an enhanced single 
      prolonged stress (ESPS) model to access the effects of early EA intervention on 
      the prevention of anxiety-like and fear learning behaviors, as well as the 
      influence of the expression of post-synaptic density protein 95 (PSD95), 
      synaptophysin (Syn), brain derived neurotrophic factor (BDNF), diacylglycerol 
      lipase alpha (DAGLalpha) and cannabinoid type 1 receptor (CB1R) in the hippocampus 
      with or without DAGLalpha or CB1R knockdown by a short hairpin RNA (shRNA) in the 
      hippocampus. Moreover, the effects of electrical stimulation with different 
      parameters on the expression of DAGLalpha and CB1R in the hippocampal astrocytes were 
      also observed. The results showed that Early EA intervention improved hippocampal 
      synaptic plasticity and ameliorated PTSD-like behaviors and also increased 
      expression of BDNF, DAGLalpha and CB1R. However, either DAGLalpha or CB1R knockdown by a 
      short hairpin RNA (shRNA) eliminated the neuroprotective effects of early EA 
      intervention. Furthermore, electrical stimulation with 2/15 Hz 1 mA elevated the 
      expression of DAGLalpha and CB1R. Altogether, our findings provide new insights 
      regarding the possibility of using early EA intervention in the prevention of 
      PTSD, and the protective effects of EA is involving the activation of DAGLalpha and 
      CB1R.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Xue, Fen
AU  - Xue F
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Xue, Shan-Shan
AU  - Xue SS
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Liu, Ling
AU  - Liu L
AD  - Institution of Neuroscience, Fourth Military Medical University, Xi'an 710032, 
      China.
FAU - Sang, Han-Fei
AU  - Sang HF
AD  - Department of Anesthesiology, Xiang'an Hospital, Xiamen University, Xiamen 
      361101, China.
FAU - Ma, Quan-Rui
AU  - Ma QR
AD  - Department of Human Anatomy and Histology and Embryology, Basic Medical College, 
      Ningxia Medical University, Yinchuan 750004, China.
FAU - Tan, Qing-Rong
AU  - Tan QR
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Wang, Hua-Ning
AU  - Wang HN
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China.
FAU - Zhou, Cui-Hong
AU  - Zhou CH
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China. Electronic address: zhouch4610@163.com.
FAU - Peng, Zheng-Wu
AU  - Peng ZW
AD  - Department of Psychiatry, Xijing Hospital, Fourth Military Medical University, 
      Xi'an 710032, China. Electronic address: pengzw@fmmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190401
PL  - England
TA  - Prog Neuropsychopharmacol Biol Psychiatry
JT  - Progress in neuro-psychopharmacology & biological psychiatry
JID - 8211617
RN  - 0 (Endocannabinoids)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Disease Models, Animal
MH  - *Electroacupuncture/methods
MH  - Endocannabinoids/*metabolism
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Maze Learning
MH  - Neuronal Plasticity
MH  - Neuropsychological Tests
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Signal Transduction
MH  - Stress Disorders, Post-Traumatic/*prevention & control/therapy
OTO - NOTNLM
OT  - Early intervention
OT  - Electroacupuncture
OT  - Endocannabinoid system
OT  - Hippocampus
OT  - PTSD
EDAT- 2019/04/05 06:00
MHDA- 2020/02/15 06:00
CRDT- 2019/04/05 06:00
PHST- 2019/01/04 00:00 [received]
PHST- 2019/03/20 00:00 [revised]
PHST- 2019/03/29 00:00 [accepted]
PHST- 2019/04/05 06:00 [pubmed]
PHST- 2020/02/15 06:00 [medline]
PHST- 2019/04/05 06:00 [entrez]
AID - S0278-5846(19)30005-3 [pii]
AID - 10.1016/j.pnpbp.2019.03.018 [doi]
PST - ppublish
SO  - Prog Neuropsychopharmacol Biol Psychiatry. 2019 Jul 13;93:171-181. doi: 
      10.1016/j.pnpbp.2019.03.018. Epub 2019 Apr 1.