PMID- 30947188
OWN - NLM
STAT- MEDLINE
DCOM- 20190809
LR  - 20190809
IS  - 1423-0267 (Electronic)
IS  - 0030-3755 (Linking)
VI  - 242
IP  - 2
DP  - 2019
TI  - Premacular Cell Proliferation Profiles in Tangential Traction 
      Vitreo-Maculopathies Suggest a Key Role for Hyalocytes.
PG  - 106-112
LID - 10.1159/000495853 [doi]
AB  - PURPOSE: To compare immunocytochemical and ultrastructural features of premacular 
      tissue surgically removed from eyes with tangential traction 
      vitreo-maculopathies. METHODS: By spectral-domain optical coherence tomography 
      (SD-OCT), premacular tissue was differentiated into premacular proliferation and 
      premacular membrane (PMM). Specimens were harvested during vitrectomy from 10 
      eyes with macular pucker, lamellar macular hole (LMH) and full-thickness macular 
      hole, and prepared for immunocytochemistry and transmission electron microscopy. 
      RESULTS: All specimens showed positive autofluorescence consistent with the 
      yellow colour of peeled tissue. Glial cells were predominantly positive in 
      premacular proliferation. Hyalocytes were the main cell type in PMM. Electron 
      microscopy revealed densely packed premacular glial cells neighbouring hyalocytes 
      and vitreous collagen strands. Myofibroblasts with features indicative of 
      contractile properties were found in PMM, exclusively. Cell composition of 
      premacular proliferation was free of contractile elements. CONCLUSION: All three 
      types of vitreo-maculopathy have similar cell constituents in their premacular 
      tissue. Cell population of premacular proliferation is not unique for LMHs. 
      Corresponding to SD-OCT, electron microscopy demonstrates hyalocytes and vitreous 
      collagen in PMMs both directly adjacent to the cellular complex of premacular 
      proliferation. Study results point to the vitreous as one important pathogenic 
      player potentially driving the degenerative cellular process at the vitreoretinal 
      interface in tangential traction vitreo-maculopathies.
CI  - (c) 2019 S. Karger AG, Basel.
FAU - Schumann, Ricarda G
AU  - Schumann RG
AD  - Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany.
FAU - Hagenau, Felix
AU  - Hagenau F
AD  - Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany.
FAU - Guenther, Stefanie R
AU  - Guenther SR
AD  - Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany.
FAU - Wolf, Armin
AU  - Wolf A
AD  - Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany.
FAU - Priglinger, Siegfried G
AU  - Priglinger SG
AD  - Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany.
FAU - Vogt, Denise
AU  - Vogt D
AD  - Department of Ophthalmology, Ludwig Maximilian University, Munich, Germany, 
      denise.vogt@med.uni-muenchen.de.
LA  - eng
PT  - Journal Article
DEP - 20190404
PL  - Switzerland
TA  - Ophthalmologica
JT  - Ophthalmologica. Journal international d'ophtalmologie. International journal of 
      ophthalmology. Zeitschrift fur Augenheilkunde
JID - 0054655
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Cell Proliferation
MH  - Epiretinal Membrane/*pathology
MH  - Female
MH  - Follow-Up Studies
MH  - Humans
MH  - Immunohistochemistry
MH  - Macula Lutea/*pathology
MH  - Male
MH  - Microscopy, Electron, Transmission
MH  - Middle Aged
MH  - Retrospective Studies
MH  - Tomography, Optical Coherence/*methods
MH  - Visual Acuity
MH  - Vitreoretinopathy, Proliferative/*pathology
MH  - Vitreous Body/*pathology
OTO - NOTNLM
OT  - Full-thickness macular hole
OT  - Hyalocytes
OT  - Immunocytochemistry
OT  - Lamellar macular hole
OT  - Premacular membrane
OT  - Premacular proliferation
OT  - Tangential traction vitreo-maculopathy
OT  - Transmission electron microscopy
OT  - Vitreoschisis
EDAT- 2019/04/05 06:00
MHDA- 2019/08/10 06:00
CRDT- 2019/04/05 06:00
PHST- 2018/07/02 00:00 [received]
PHST- 2018/11/27 00:00 [accepted]
PHST- 2019/04/05 06:00 [pubmed]
PHST- 2019/08/10 06:00 [medline]
PHST- 2019/04/05 06:00 [entrez]
AID - 000495853 [pii]
AID - 10.1159/000495853 [doi]
PST - ppublish
SO  - Ophthalmologica. 2019;242(2):106-112. doi: 10.1159/000495853. Epub 2019 Apr 4.