PMID- 30948095 OWN - NLM STAT- MEDLINE DCOM- 20190725 LR - 20190725 IS - 1873-0191 (Electronic) IS - 0928-4931 (Linking) VI - 100 DP - 2019 Jul TI - An injectable conductive Gelatin-PANI hydrogel system serves as a promising carrier to deliver BMSCs for Parkinson's disease treatment. PG - 584-597 LID - S0928-4931(18)31252-9 [pii] LID - 10.1016/j.msec.2019.03.024 [doi] AB - Parkinson's disease (PD) is a long-term neurodegenerative disorders that characterized by a progressive loss of dopaminergic neurons in substantia nigra pars compacta (SNc). Bone marrow stromal cells (BMSCs) are promising therapeutic agents for neurodegenerative disease due to their multipotent capacity. To promote the potential therapeutic effect of BMSCs on PD, we developed an injectable Gelatin-PANI hydrogels as a novel carrier for delivering BMSCs to the SNc region in mice with PD by stereotactic injection. Histology results showed that the BMSCs-loaded hydrogels lead to increased numbers of tyrosine hydroxylase positive (TH(+)) dopaminergic neurons and fibers in the SNc and striatum, and increased expression of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) in the SNc. Meanwhile, rotarod and open field evaluation demonstrated BMSCs-loaded hydrogels significantly improved the behavioral performance of PD mice. Importantly, BMSCs-loaded hydrogels imparted more sustained protective effects than BMSCs alone in PD mice. Overall, the current data indicate that the hydrogel serves as a promising carrier to deliver BMSCs to the SNc for the treatment of PD. CI - Copyright (c) 2019. Published by Elsevier B.V. FAU - Xue, Jinhua AU - Xue J AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Liu, Yutong AU - Liu Y AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Darabi, Mohammad Ali AU - Darabi MA AD - Departments of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba and Manitoba Institute of Child Health, Winnipeg, MB R3T 2N2, Canada. FAU - Tu, Genghong AU - Tu G AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Huang, Lu AU - Huang L AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Ying, Li AU - Ying L AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Xiao, Bin AU - Xiao B AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Wu, Yue AU - Wu Y AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. FAU - Xing, Malcolm AU - Xing M AD - Departments of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba and Manitoba Institute of Child Health, Winnipeg, MB R3T 2N2, Canada. Electronic address: Malcolm.xing@umanitoba.ca. FAU - Zhang, Lin AU - Zhang L AD - Key Laboratory of Construction and Detection in Tissue Engineering of Guangdong Province, Department of Histology and Embryology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. Electronic address: zlilyzh@smu.edu.cn. FAU - Zhang, Lu AU - Zhang L AD - Key Laboratory of Functional Proteomics of Guangdong Province, Key Laboratory of Mental Health of the Ministry of Education, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, China. Electronic address: zhanglu@smu.edu.cn. LA - eng PT - Journal Article DEP - 20190308 PL - Netherlands TA - Mater Sci Eng C Mater Biol Appl JT - Materials science & engineering. C, Materials for biological applications JID - 101484109 RN - 0 (Aniline Compounds) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Delayed-Action Preparations) RN - 0 (Drug Carriers) RN - 0 (Glial Cell Line-Derived Neurotrophic Factor) RN - 0 (Hydrogels) RN - 0 (polyaniline) RN - 9000-70-8 (Gelatin) RN - 9P21XSP91P (1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine) MH - 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine MH - Aniline Compounds/chemical synthesis/chemistry MH - Animals MH - Brain-Derived Neurotrophic Factor/pharmacology MH - Cell Survival MH - Delayed-Action Preparations/pharmacology MH - Dopaminergic Neurons/pathology MH - Drug Carriers/*chemistry MH - *Electric Conductivity MH - Gelatin/chemical synthesis/*chemistry MH - Glial Cell Line-Derived Neurotrophic Factor/pharmacology MH - Hydrogels/chemical synthesis/*chemistry MH - *Injections MH - Male MH - *Mesenchymal Stem Cell Transplantation MH - Mesenchymal Stem Cells/*cytology MH - Mice, Inbred C57BL MH - Parkinson Disease/pathology/*therapy MH - Rheology MH - Substantia Nigra/pathology OTO - NOTNLM OT - Bone marrow stromal cells (BMSCs) OT - Hydrogel OT - PANI OT - Parkinson's disease (PD) OT - Substantia nigra pars compacta (SNc) EDAT- 2019/04/06 06:00 MHDA- 2019/07/26 06:00 CRDT- 2019/04/06 06:00 PHST- 2018/05/08 00:00 [received] PHST- 2018/12/27 00:00 [revised] PHST- 2019/03/07 00:00 [accepted] PHST- 2019/04/06 06:00 [entrez] PHST- 2019/04/06 06:00 [pubmed] PHST- 2019/07/26 06:00 [medline] AID - S0928-4931(18)31252-9 [pii] AID - 10.1016/j.msec.2019.03.024 [doi] PST - ppublish SO - Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:584-597. doi: 10.1016/j.msec.2019.03.024. Epub 2019 Mar 8.