PMID- 30949151
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240417
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 10
DP  - 2019
TI  - Metabolic Peculiarities of Paracoccidioides brasiliensis Dimorphism as 
      Demonstrated by iTRAQ Labeling Proteomics.
PG  - 555
LID - 10.3389/fmicb.2019.00555 [doi]
LID - 555
AB  - Paracoccidioidomycosis (PCM), a systemic mycosis with a high incidence in Latin 
      America, is caused by thermodimorphic fungi of the Paracoccidioides genus. The 
      contact with host occurs by the inhalation of conidia or mycelial propagules 
      which once reaching the pulmonary alveoli differentiate into yeast cells. This 
      transition process is vital in the pathogenesis of PCM allowing the fungus 
      survival in the host. Thus, the present work performed a comparative proteome 
      analysis of mycelia, mycelia-to-yeast transition, and yeast cells of 
      Paracoccidioides brasiliensis. For that, tryptic peptides were labeled with iTRAQ 
      and identified by LC-MS/MS and computational data analysis, which allowed the 
      identification of 312 proteins differentially expressed in different 
      morphological stages. Data showed that P. brasiliensis yeast cells preferentially 
      employ aerobic beta-oxidation and the tricarboxylic acid cycle accompanied by 
      oxidative phosphorylation for ATP production, in comparison to mycelia and the 
      transition from mycelia-to-yeast cells. Furthermore, yeast cells show a metabolic 
      reprogramming in amino acid metabolism and in the induction of virulence 
      determinants and heat shock proteins allowing adaptation to environmental 
      conditions during the increase of the temperature. In opposite of that, the 
      alcoholic fermentation found to P. lutzii, at least under laboratory conditions, 
      is strongly favored in mycelium compared to yeast cells. Thereby, the data 
      strongly support substantial metabolic differences among members of the 
      Paracoccidioides complex, when comparing the saprobiotic mycelia and the yeast 
      parasitic phases.
FAU - Araujo, Danielle Silva
AU  - Araujo DS
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Brazil.
AD  - Laboratorio de Bioquimica e Quimica de Proteinas, Instituto de Ciencias 
      Biologicas, Universidade de Brasilia, Brasilia, Brazil.
AD  - Faculdade Unida de Campinas, Goiania, Brazil.
FAU - Pereira, Maristela
AU  - Pereira M
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Brazil.
FAU - Portis, Igor Godinho
AU  - Portis IG
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Brazil.
FAU - Dos Santos Junior, Agenor de Castro Moreira
AU  - Dos Santos Junior ACM
AD  - Laboratorio de Bioquimica e Quimica de Proteinas, Instituto de Ciencias 
      Biologicas, Universidade de Brasilia, Brasilia, Brazil.
FAU - Fontes, Wagner
AU  - Fontes W
AD  - Laboratorio de Bioquimica e Quimica de Proteinas, Instituto de Ciencias 
      Biologicas, Universidade de Brasilia, Brasilia, Brazil.
FAU - de Sousa, Marcelo Valle
AU  - de Sousa MV
AD  - Laboratorio de Bioquimica e Quimica de Proteinas, Instituto de Ciencias 
      Biologicas, Universidade de Brasilia, Brasilia, Brazil.
FAU - Assuncao, Leandro do Prado
AU  - Assuncao LDP
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Brazil.
FAU - Baeza, Lilian Cristiane
AU  - Baeza LC
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Brazil.
FAU - Bailao, Alexandre Mello
AU  - Bailao AM
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Brazil.
FAU - Ricart, Carlos Andre Ornelas
AU  - Ricart CAO
AD  - Laboratorio de Bioquimica e Quimica de Proteinas, Instituto de Ciencias 
      Biologicas, Universidade de Brasilia, Brasilia, Brazil.
FAU - Brock, Matthias
AU  - Brock M
AD  - Fungal Biology and Genetics Group, University of Nottingham, Nottingham, United 
      Kingdom.
FAU - Soares, Celia Maria de Almeida
AU  - Soares CMA
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, Goiania, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20190320
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC6436475
OTO - NOTNLM
OT  - dimorphism
OT  - iTRAQ
OT  - neglected disease
OT  - paracoccidioidomycosis
OT  - proteomics
EDAT- 2019/04/06 06:00
MHDA- 2019/04/06 06:01
PMCR- 2019/03/20
CRDT- 2019/04/06 06:00
PHST- 2019/01/21 00:00 [received]
PHST- 2019/03/04 00:00 [accepted]
PHST- 2019/04/06 06:00 [entrez]
PHST- 2019/04/06 06:00 [pubmed]
PHST- 2019/04/06 06:01 [medline]
PHST- 2019/03/20 00:00 [pmc-release]
AID - 10.3389/fmicb.2019.00555 [doi]
PST - epublish
SO  - Front Microbiol. 2019 Mar 20;10:555. doi: 10.3389/fmicb.2019.00555. eCollection 
      2019.