PMID- 30951888
OWN - NLM
STAT- MEDLINE
DCOM- 20200206
LR  - 20200206
IS  - 1769-714X (Electronic)
IS  - 1286-4579 (Linking)
VI  - 21
IP  - 8-9
DP  - 2019 Oct-Nov
TI  - Identification and characterization of Paracoccidioides lutzii proteins 
      interacting with macrophages.
PG  - 401-411
LID - S1286-4579(19)30046-2 [pii]
LID - 10.1016/j.micinf.2019.03.002 [doi]
AB  - Paracoccidioidomycosis (PCM), caused by thermodimorphic fungi of the 
      Paracoccidioides genus, is a systemic disorder that involves the lungs and other 
      organs. The adherence of pathogenic microorganisms to host tissues is an 
      essential event in the onset of colonization and spread. The host-pathogen 
      interaction is a complex interplay between the defense mechanisms of the host and 
      the efforts of pathogenic microorganisms to colonize it. Therefore, the 
      identification of fungi proteins interacting with host proteins is an important 
      step understanding the survival strategies of the fungus within the host. In this 
      paper, we used affinity chromatography based on surface proteomics (ACSP) to 
      investigate the interactions of pathogen proteins with host surface molecules. 
      Paracoccidioides lutzii extracts enriched of surface proteins were captured by 
      chromatographic resin, which was immobilized with macrophage cell surface 
      proteins, and identified by mass spectrometry. A total of 215 proteins of 
      P. lutzii were identified interacting with macrophage proteins. In silico 
      analysis classified those proteins according to the presence of sites for N- and 
      O-glycosylation and secretion by classical and non-classical pathways. Serine 
      proteinase (SP) and fructose-1,6-bisphosphate aldolase (FBA) were identified in 
      our proteomics analysis. Immunolocalization assay and flow cytometry both showed 
      an increase in the expression of these two proteins during host-pathogen 
      interaction.
CI  - Copyright (c) 2019 Institut Pasteur. Published by Elsevier Masson SAS. All rights 
      reserved.
FAU - Tomazett, Mariana Vieira
AU  - Tomazett MV
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, 74001-970, Goiania, Goias, Brazil. Electronic address: 
      marianatomazett@gmail.com.
FAU - Baeza, Lilian Cristiane
AU  - Baeza LC
AD  - Centro de Ciencias Medicas e Farmaceuticas, Universidade Estadual do Oeste do 
      Parana, 85819-110, Cascavel, Parana, Brazil. Electronic address: 
      lilianbaeza@gmail.com.
FAU - Paccez, Juliano Domiraci
AU  - Paccez JD
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, 74001-970, Goiania, Goias, Brazil. Electronic address: 
      julianopaccez@gmail.com.
FAU - Parente-Rocha, Juliana Alves
AU  - Parente-Rocha JA
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, 74001-970, Goiania, Goias, Brazil. Electronic address: 
      juparente@gmail.com.
FAU - Ribeiro-Dias, Fatima
AU  - Ribeiro-Dias F
AD  - Laboratorio de Imunidade Natural, Instituto de Patologia Tropical e Saude 
      Publica, Universidade Federal de Goias, 74605-050, Goiania, Goias, Brazil. 
      Electronic address: fatimardias@gmail.com.
FAU - de Almeida Soares, Celia Maria
AU  - de Almeida Soares CM
AD  - Laboratorio de Biologia Molecular, Instituto de Ciencias Biologicas, Universidade 
      Federal de Goias, 74001-970, Goiania, Goias, Brazil. Electronic address: 
      cmasoares@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190402
PL  - France
TA  - Microbes Infect
JT  - Microbes and infection
JID - 100883508
RN  - 0 (Fungal Proteins)
RN  - 0 (Immobilized Proteins)
RN  - 0 (Membrane Proteins)
RN  - EC 3.4.- (Serine Proteases)
RN  - EC 4.1.2.13 (Fructose-Bisphosphate Aldolase)
SB  - IM
MH  - Animals
MH  - Cell Wall/chemistry/metabolism
MH  - Fructose-Bisphosphate Aldolase/genetics/metabolism
MH  - Fungal Proteins/genetics/*metabolism
MH  - *Host-Pathogen Interactions
MH  - Immobilized Proteins/metabolism
MH  - Macrophages/*metabolism/microbiology
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Paracoccidioides/metabolism/*physiology
MH  - Protein Binding
MH  - Proteomics
MH  - RAW 264.7 Cells
MH  - Serine Proteases/genetics/metabolism
OTO - NOTNLM
OT  - Fructose-1,6-Bisphosphate aldolase
OT  - Interaction
OT  - Macrophages
OT  - Paracoccidioides lutzii
OT  - Serine proteinase
EDAT- 2019/04/06 06:00
MHDA- 2020/02/07 06:00
CRDT- 2019/04/06 06:00
PHST- 2019/01/09 00:00 [received]
PHST- 2019/03/21 00:00 [revised]
PHST- 2019/03/22 00:00 [accepted]
PHST- 2019/04/06 06:00 [pubmed]
PHST- 2020/02/07 06:00 [medline]
PHST- 2019/04/06 06:00 [entrez]
AID - S1286-4579(19)30046-2 [pii]
AID - 10.1016/j.micinf.2019.03.002 [doi]
PST - ppublish
SO  - Microbes Infect. 2019 Oct-Nov;21(8-9):401-411. doi: 10.1016/j.micinf.2019.03.002. 
      Epub 2019 Apr 2.