PMID- 30954278
OWN - NLM
STAT- MEDLINE
DCOM- 20200330
LR  - 20200330
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Linking)
VI  - 332
DP  - 2019 Jul 15
TI  - Dopamine inhibits human CD8+ Treg function through D(1)-like dopaminergic 
      receptors.
PG  - 233-241
LID - S0165-5728(18)30578-2 [pii]
LID - 10.1016/j.jneuroim.2019.02.007 [doi]
AB  - CD8+ T regulatory/suppressor cells (Treg) affect peripheral tolerance and may be 
      involved in autoimmune diseases as well as in cancer. In view of our previous 
      data showing the ability of DA to affect adaptive immune responses, we 
      investigated the dopaminergic phenotype of human CD8+ Treg as well as the ability 
      of DA to affect their generation and activity. Results show that CD8+ T cells 
      express both D(1)-like and D(2)-like dopaminergic receptors (DR), tyrosine 
      hydroxylase (TH), the rate-limiting enzyme in the synthesis of DA, and vesicular 
      monoamine transporter (VMAT) 2 and contain high levels of intracellular DA. 
      Preferential upregulation of DR mRNA levels in the CD8+CD28- T cell compartment 
      occurs during generation of CD8+ Treg, which is reduced by DA and by the 
      D(1)-like DR agonist SKF-38393. DA and SKF-38393 also reduce the suppressive 
      activity of CD8+ Treg on human peripheral blood mononuclear cells. Treg are 
      crucial for tumor escape from the host immune system, thus the ability of DA to 
      inhibits Treg function supports dopaminergic pathways as a druggable targets to 
      develop original and innovative antitumor strategies.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - Nasi, Giorgia
AU  - Nasi G
AD  - Center of Excellence for Biomedical Research/Department of Internal Medicine, 
      Clinical Immunology Unit, Clinical Immunology Unit, University of Genoa, Genoa, 
      Italy; Biotherapy Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy. 
      Electronic address: gfilaci@unige.it.
FAU - Ahmed, Tanzeel
AU  - Ahmed T
AD  - Center for Research in Medical Pharmacology, University of Insubria, Varese, 
      Italy.
FAU - Rasini, Emanuela
AU  - Rasini E
AD  - Center for Research in Medical Pharmacology, University of Insubria, Varese, 
      Italy.
FAU - Fenoglio, Daniela
AU  - Fenoglio D
AD  - Center of Excellence for Biomedical Research/Department of Internal Medicine, 
      Clinical Immunology Unit, Clinical Immunology Unit, University of Genoa, Genoa, 
      Italy; Biotherapy Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
FAU - Marino, Franca
AU  - Marino F
AD  - Center for Research in Medical Pharmacology, University of Insubria, Varese, 
      Italy. Electronic address: franca.marino@uninsubria.it.
FAU - Filaci, Gilberto
AU  - Filaci G
AD  - Center of Excellence for Biomedical Research/Department of Internal Medicine, 
      Clinical Immunology Unit, Clinical Immunology Unit, University of Genoa, Genoa, 
      Italy; Biotherapy Unit, IRCCS Ospedale Policlinico San Martino, Genoa, Italy.
FAU - Cosentino, Marco
AU  - Cosentino M
AD  - Center for Research in Medical Pharmacology, University of Insubria, Varese, 
      Italy. Electronic address: franca.marino@uninsubria.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190309
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Dopamine D1)
RN  - 0 (SLC18A2 protein, human)
RN  - 0 (Vesicular Monoamine Transport Proteins)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Dopamine/*pharmacology
MH  - Flow Cytometry
MH  - Humans
MH  - Neuroimmunomodulation/physiology
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Dopamine D1/analysis/biosynthesis/*drug effects/genetics
MH  - T-Lymphocytes, Regulatory/*drug effects/metabolism
MH  - Tyrosine 3-Monooxygenase/biosynthesis/genetics
MH  - Vesicular Monoamine Transport Proteins/biosynthesis/genetics
OTO - NOTNLM
OT  - CD8+ Treg
OT  - Dopamine
OT  - Dopaminergic receptor
EDAT- 2019/04/08 06:00
MHDA- 2020/03/31 06:00
CRDT- 2019/04/08 06:00
PHST- 2018/12/19 00:00 [received]
PHST- 2019/02/06 00:00 [revised]
PHST- 2019/02/15 00:00 [accepted]
PHST- 2019/04/08 06:00 [pubmed]
PHST- 2020/03/31 06:00 [medline]
PHST- 2019/04/08 06:00 [entrez]
AID - S0165-5728(18)30578-2 [pii]
AID - 10.1016/j.jneuroim.2019.02.007 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2019 Jul 15;332:233-241. doi: 10.1016/j.jneuroim.2019.02.007. 
      Epub 2019 Mar 9.