PMID- 30954462
OWN - NLM
STAT- MEDLINE
DCOM- 20200603
LR  - 20200603
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 269
DP  - 2019 Jul
TI  - Development of a cucumber green mottle mosaic virus-based expression vector for 
      the production in cucumber of neutralizing epitopes against a devastating animal 
      virus.
PG  - 18-25
LID - S0166-0934(18)30332-X [pii]
LID - 10.1016/j.jviromet.2019.04.006 [doi]
AB  - Virus-based expression systems have been widely exploited for the production of 
      recombinant proteins in plants during the last thirty years. Advances in 
      technology have boosted scale-up manufacturing of plant-made pharmaceuticals to 
      high levels, via the complementation of transient expression and viral vectors. 
      This combination allows proteins of interest to be produced in plants within a 
      matter of days and thus, is well suited for the development of plant-made 
      vaccines or therapeutics against emerging infectious diseases and potential 
      bioterrorism agents. Several plant-based products are currently in varying stages 
      of clinical development. To investigate the viability of virus-based expression 
      systems for plant-made vaccines against porcine reproductive and respiratory 
      syndrome virus (PRRSV), the most devastating threat to the pork industry in 
      Canada, we cloned the full-length genome of a cucumber green mottle mosaic virus 
      (CGMMV) isolate and developed a CGMMV-based expression vector. We further 
      employed this vector to express the neutralizing epitope (NE) of PRRSV 
      glycoprotein 5 (GP5) in cucumber leaves via agroinfiltration. The coding region 
      of the GP5 NE was inserted downstream of the open reading frame for coat protein 
      (CP) and expressed by a readthrough mechanism. The chimeric virus particles were 
      stable and the expression levels reached as high as 35.84 mg/kg of cucumber leaf 
      fresh weight. This study offers a promising solution to the production of a low 
      cost, versatile and robust vaccine for oral administration against PRRSV through 
      a chimeric virus particle display system.
CI  - Crown Copyright (c) 2019. Published by Elsevier B.V. All rights reserved.
FAU - Tran, Hong Hanh
AU  - Tran HH
AD  - London Research and Development Centre, Agriculture and Agri-Food Canada, London, 
      Ontario, N5V 4T3, Canada; Department of Biology, Western University, London, 
      Ontario, N6A 5B7, Canada.
FAU - Chen, Bin
AU  - Chen B
AD  - London Research and Development Centre, Agriculture and Agri-Food Canada, London, 
      Ontario, N5V 4T3, Canada; Department of Biology, Western University, London, 
      Ontario, N6A 5B7, Canada.
FAU - Chen, Hui
AU  - Chen H
AD  - London Research and Development Centre, Agriculture and Agri-Food Canada, London, 
      Ontario, N5V 4T3, Canada; Department of Biology, Western University, London, 
      Ontario, N6A 5B7, Canada.
FAU - Menassa, Rima
AU  - Menassa R
AD  - London Research and Development Centre, Agriculture and Agri-Food Canada, London, 
      Ontario, N5V 4T3, Canada.
FAU - Hao, Xiuming
AU  - Hao X
AD  - Harrow Research and Development Centre, Agriculture and Agri-Food Canada, 2585 
      County Road 20, Harrow, Ontario, N0R 1G0, Canada.
FAU - Bernards, Mark
AU  - Bernards M
AD  - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada.
FAU - Huner, Norman P A
AU  - Huner NPA
AD  - Department of Biology, Western University, London, Ontario, N6A 5B7, Canada.
FAU - Wang, Aiming
AU  - Wang A
AD  - London Research and Development Centre, Agriculture and Agri-Food Canada, London, 
      Ontario, N5V 4T3, Canada; Department of Biology, Western University, London, 
      Ontario, N6A 5B7, Canada. Electronic address: Aiming.Wang@CANADA.CA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190405
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Epitopes)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
RN  - Cucumber green mottle mosaic virus
SB  - IM
MH  - Animals
MH  - Cucumis sativus/*metabolism/virology
MH  - Epitopes/*immunology
MH  - *Genetic Vectors
MH  - Genome, Viral
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/immunology
MH  - RNA, Viral
MH  - Swine/virology
MH  - Tobamovirus/*genetics
MH  - Viral Envelope Proteins/genetics/immunology
MH  - Viral Vaccines/*immunology
OTO - NOTNLM
OT  - CGMMV
OT  - Molecular farming
OT  - PRRSV
OT  - Particle display
OT  - Virus vector
EDAT- 2019/04/08 06:00
MHDA- 2020/06/04 06:00
CRDT- 2019/04/08 06:00
PHST- 2018/06/29 00:00 [received]
PHST- 2019/04/02 00:00 [revised]
PHST- 2019/04/04 00:00 [accepted]
PHST- 2019/04/08 06:00 [pubmed]
PHST- 2020/06/04 06:00 [medline]
PHST- 2019/04/08 06:00 [entrez]
AID - S0166-0934(18)30332-X [pii]
AID - 10.1016/j.jviromet.2019.04.006 [doi]
PST - ppublish
SO  - J Virol Methods. 2019 Jul;269:18-25. doi: 10.1016/j.jviromet.2019.04.006. Epub 
      2019 Apr 5.