PMID- 30956681
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220408
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2019
DP  - 2019
TI  - Adenosine A2A Receptor Mediates Inhibition of Synovitis and Osteoclastogenesis 
      after Electroacupuncture in Rats with Collagen-Induced Arthritis.
PG  - 4617464
LID - 10.1155/2019/4617464 [doi]
LID - 4617464
AB  - BACKGROUND: This study was to investigate the role of adenosine A2A receptors 
      (A2AR) in inhibiting the effect of electroacupuncture (EA) on osteoclastogenesis 
      in collagen-induced arthritis (CIA). METHODS: Wistar rats were divided into four 
      groups: sham-control group, CIA-control group, CIA-EA group, and CIA-EA-SCH58261 
      (A2AR antagonist) group. We detected tumor necrosis factor-alpha (TNF-alpha), nuclear 
      transcription factor-kappaB (NF-kappaB), receptor activator of NF-kappaB ligand (RANKL), 
      protein kinase A (PKA), and extracellular regulatory protein kinase 1/2 (ERK1/2) 
      in peripheral blood by ELISA. PKA, ERK1/2, and NF-kappaB in ankle joints were 
      determined by western blotting. We evaluated the arthritis damage by histological 
      examination and determined the number of osteoclasts by tartrate-resistant acid 
      phosphatase (TRAP) staining. RESULTS: EA treatment downregulated the expression 
      of TNF-alpha, RANKL, PKA, ERK1/2, and NF-kappaB in peripheral blood but increased the 
      levels of PKA and ERK1/2 in ankle joints. Importantly, EA treatment reduced bone 
      erosion as evidenced by the histological findings and inhibited 
      osteoclastogenesis as revealed by TRAP staining. All these effects of the EA 
      treatment were reversed by combining EA treatment with the A2AR antagonist 
      SCH58261. CONCLUSION: Our data suggest that EA treatment activated A2AR. The 
      effects of the A2AR antagonist SCH58261 suggest that the inhibition of osteoclast 
      formation, the inhibition of TNF-alpha, RANKL, and NF-kappaB expression, and the increase 
      of ERK1/2 are all dependent on this EA-induced A2AR activation. It is therefore 
      likely that these pathways with clearly defined roles in inflammation and bone 
      erosion are at least partially involved in the mediation of the inhibition of 
      synovitis and osteoclast formation induced by EA.
FAU - Du, Zhong-Heng
AU  - Du ZH
AD  - Department of Acupuncture, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Zhang, Chun-Wu
AU  - Zhang CW
AD  - Department of Traditional Chinese Orthopedics & Traumatology, The First 
      Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, 
      China.
FAU - Xie, Wen-Xia
AU  - Xie WX
AD  - Department of Acupuncture, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Chen, Yong
AU  - Chen Y
AD  - Department of Acupuncture, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Cong, Wen-Jie
AU  - Cong WJ
AD  - Department of Acupuncture, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Wang, Ze-Dong
AU  - Wang ZD
AD  - Department of Acupuncture, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Wang, En-Pei
AU  - Wang EP
AD  - Department of Acupuncture, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Wu, Guang-Yu
AU  - Wu GY
AUID- ORCID: 0000-0002-8273-1741
AD  - Department of Traumatology, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
FAU - Ye, Tian-Shen
AU  - Ye TS
AUID- ORCID: 0000-0003-1745-8918
AD  - Department of Acupuncture, The First Affiliated Hospital of Wenzhou Medical 
      University, Wenzhou, Zhejiang 325000, China.
LA  - eng
PT  - Journal Article
DEP - 20190306
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC6431381
EDAT- 2019/04/09 06:00
MHDA- 2019/04/09 06:01
PMCR- 2019/03/06
CRDT- 2019/04/09 06:00
PHST- 2019/01/12 00:00 [received]
PHST- 2019/02/26 00:00 [accepted]
PHST- 2019/04/09 06:00 [entrez]
PHST- 2019/04/09 06:00 [pubmed]
PHST- 2019/04/09 06:01 [medline]
PHST- 2019/03/06 00:00 [pmc-release]
AID - 10.1155/2019/4617464 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2019 Mar 6;2019:4617464. doi: 
      10.1155/2019/4617464. eCollection 2019.