PMID- 30958820
OWN - NLM
STAT- MEDLINE
DCOM- 20191217
LR  - 20240413
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 4
DP  - 2019
TI  - Heat shock in C. elegans induces downstream of gene transcription and 
      accumulation of double-stranded RNA.
PG  - e0206715
LID - 10.1371/journal.pone.0206715 [doi]
LID - e0206715
AB  - We observed that heat shock of Caenorhabditis elegans leads to the formation of 
      nuclear double-stranded RNA (dsRNA) foci, detectable with a dsRNA-specific 
      monoclonal antibody. These foci significantly overlap with nuclear HSF-1 
      granules. To investigate the molecular mechanism(s) underlying dsRNA foci 
      formation, we used RNA-seq to globally characterize total RNA and 
      immunoprecipitated dsRNA from control and heat shocked worms. We find a subset of 
      both sense and antisense transcripts enriched in the dsRNA pool by heat shock 
      overlap with dsRNA transcripts enriched by deletion of tdp-1, which encodes the 
      C. elegans ortholog of TDP-43. Interestingly, transcripts involved in translation 
      are over-represented in the dsRNAs induced by either heat shock or deletion of 
      tdp-1. Also enriched in the dsRNA transcripts are sequences downstream of 
      annotated genes (DoGs), which we globally quantified with a new algorithm. To 
      validate these observations, we used fluorescence in situ hybridization (FISH) to 
      confirm both antisense and downstream of gene transcription for eif-3.B, one of 
      the affected loci we identified.
FAU - Melnick, Marko
AU  - Melnick M
AUID- ORCID: 0000-0001-8616-3019
AD  - Integrative Physiology, University of Colorado, Boulder, Colorado, United States 
      of America.
FAU - Gonzales, Patrick
AU  - Gonzales P
AD  - Integrative Physiology, University of Colorado, Boulder, Colorado, United States 
      of America.
FAU - Cabral, Joseph
AU  - Cabral J
AUID- ORCID: 0000-0003-4733-6612
AD  - Integrative Physiology, University of Colorado, Boulder, Colorado, United States 
      of America.
FAU - Allen, Mary A
AU  - Allen MA
AD  - BioFrontiers Institute, University of Colorado, Boulder, Colorado, United States 
      of America.
FAU - Dowell, Robin D
AU  - Dowell RD
AD  - BioFrontiers Institute and Department of Molecular, Cellular and Developmental 
      Biology, University of Colorado, Boulder, Colorado, United States of America.
FAU - Link, Christopher D
AU  - Link CD
AD  - Integrative Physiology, University of Colorado, Boulder, Colorado, United States 
      of America.
AD  - Institute for Behavioral Genetics, University of Colorado, Boulder, Colorado, 
      United States of America.
LA  - eng
GR  - R01 NS063964/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20190408
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Helminth)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (TDP-1 protein, C elegans)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/cytology/genetics/*metabolism
MH  - Caenorhabditis elegans Proteins/genetics/metabolism
MH  - Cell Nucleus/genetics/*metabolism
MH  - *Heat-Shock Response
MH  - RNA, Double-Stranded/genetics/*metabolism
MH  - RNA, Helminth/genetics/*metabolism
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - *Transcription, Genetic
PMC - PMC6453478
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/04/09 06:00
MHDA- 2019/12/18 06:00
PMCR- 2019/04/08
CRDT- 2019/04/09 06:00
PHST- 2018/10/15 00:00 [received]
PHST- 2019/03/25 00:00 [accepted]
PHST- 2019/04/09 06:00 [entrez]
PHST- 2019/04/09 06:00 [pubmed]
PHST- 2019/12/18 06:00 [medline]
PHST- 2019/04/08 00:00 [pmc-release]
AID - PONE-D-18-29477 [pii]
AID - 10.1371/journal.pone.0206715 [doi]
PST - epublish
SO  - PLoS One. 2019 Apr 8;14(4):e0206715. doi: 10.1371/journal.pone.0206715. 
      eCollection 2019.