PMID- 30961693
OWN - NLM
STAT- MEDLINE
DCOM- 20210609
LR  - 20210609
IS  - 1533-4880 (Print)
IS  - 1533-4880 (Linking)
VI  - 19
IP  - 9
DP  - 2019 Sep 1
TI  - Promoting Osteogenesis of Human Umbilical Cord Mesenchymal Stem Cells Using 1alpha, 
      25-(OH)(2)D(3) to Change Intracellular Calcium Concentration.
PG  - 5435-5440
LID - 10.1166/jnn.2019.16509 [doi]
AB  - Vitamin D plays a major role in the regulation of calcium homeostasis and affects 
      bone metabolism. There is currently limited detailed knowledge about the vitamin 
      D endocrine system in human bone cells. Here, we investigated the direct effects 
      of 1alpha, 25-dihydroxyvitamin D(3) (1alpha, 25-(OH)(2)D(3) or 'VD3') on osteogenesis of human 
      umbilical cord mesenchymal stem cells (HUCMSCs). We also studied the impact of 
      VD3 on intracellular Ca(2+) concentrations in osteogenic cells. The results of 
      alizarin red staining and alkaline phosphatase activity tests showed that VD3 
      could not induce osteogenic differentiation in HUCMSCs. However, addition of VD3 
      to the osteogenic differentiationinducing medium could promote HUCMSC to 
      differentiate into osteoblasts. Calcium imaging showed that the addition of VD3 
      increased intracellular Ca(2+) concentrations in osteogenic HUCMSCs. Thus, we 
      concluded that adding VD3 increased intracellular Ca(2+) concentrations in 
      osteogenic HUCMSCs and promoted their osteogenesis.
FAU - Wang, Fei
AU  - Wang F
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Cheng, Na
AU  - Cheng N
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Chen, Jie
AU  - Chen J
AD  - Hunan HuiYiSen Cell Gene Engineering Co. Ltd., Changsha 410008, China.
FAU - Yang, Yuchen
AU  - Yang Y
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Huang, Rongqi
AU  - Huang R
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Huang, Hualin
AU  - Huang H
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Lin, Zuoxian
AU  - Lin Z
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Tian, Chao
AU  - Tian C
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Zhao, Huifang
AU  - Zhao H
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Long, Yan
AU  - Long Y
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
FAU - Li, Zhiyuan
AU  - Li Z
AD  - Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of 
      Stem Cell and Regenerative Medicine, Guangzhou Institute of Biomedicine and 
      Health, Chinese Academy of Sciences, Guangzhou 510530, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nanosci Nanotechnol
JT  - Journal of nanoscience and nanotechnology
JID - 101088195
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - *Osteogenesis
MH  - Umbilical Cord
EDAT- 2019/04/10 06:00
MHDA- 2021/06/10 06:00
CRDT- 2019/04/10 06:00
PHST- 2019/04/10 06:00 [entrez]
PHST- 2019/04/10 06:00 [pubmed]
PHST- 2021/06/10 06:00 [medline]
AID - 10.1166/jnn.2019.16509 [doi]
PST - ppublish
SO  - J Nanosci Nanotechnol. 2019 Sep 1;19(9):5435-5440. doi: 10.1166/jnn.2019.16509.