PMID- 30965560
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20190411
LR  - 20210413
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 19
IP  - 7
DP  - 2019 Apr 8
TI  - Use of Surface-Enhanced Raman Scattering (SERS) Probes to Detect Fatty Acid 
      Receptor Activity in a Microfluidic Device.
LID - 10.3390/s19071663 [doi]
LID - 1663
AB  - In this study, 4-mercaptobenzoic acid (MBA)-Au nanorods conjugated with a GPR120 
      antibody were developed as a highly sensitive surface-enhanced Raman spectroscopy 
      (SERS) probe, and were applied to detect the interaction of fatty acids (FA) and 
      their cognate receptor, GPR120, on the surface of human embryonic kidney cells 
      (HEK293-GPRR120) cultured in a polydimethylsiloxane (PDMS) microfluidic device. 
      Importantly, the two dominant characteristic SERS peaks of the Raman reporter 
      molecule MBA, 1078 cm(-1) and 1581 cm(-1), do not overlap with the main Raman 
      peaks from the PDMS substrate when the appropriate spectral scanning range is 
      selected, which effectively avoided the interference from the PDMS background 
      signals. The proposed microfluidic device consisted of two parts, that is, the 
      concentration gradient generator (CGG) and the cell culture well array. The CGG 
      part was fabricated to deliver five concentrations of FA simultaneously. A high 
      aspect ratio well structure was designed to address the problem of HEK cells 
      vulnerable to shear flow. The results showed a positive correlation between the 
      SERS peak intensity and the FA concentrations. This work, for the first time, 
      achieved the simultaneous monitoring of the Raman spectra of cells and the 
      responses of the receptor in the cells upon the addition of fatty acid. The 
      development of this method also provides a platform for the monitoring of cell 
      membrane receptors on single-cell analysis using SERS in a PDMS-based 
      microfluidic device.
FAU - Zhang, Han
AU  - Zhang H
AUID- ORCID: 0000-0002-9991-9409
AD  - Department of Biological Engineering, Utah State University, Logan, UT 
      84322-4105, USA. han.zhang1985@aggiemail.usu.edu.
FAU - Zhang, Wei
AU  - Zhang W
AUID- ORCID: 0000-0002-1326-6700
AD  - Department of Biological Engineering, Utah State University, Logan, UT 
      84322-4105, USA. wei.zhang@aggiemail.usu.edu.
FAU - Xiao, Lifu
AU  - Xiao L
AUID- ORCID: 0000-0002-0320-2076
AD  - Department of Biological Engineering, Utah State University, Logan, UT 
      84322-4105, USA. lfxiao0517@gmail.com.
FAU - Liu, Yan
AU  - Liu Y
AD  - Department of Internal Medicine, University Central Florida, Orlando, FL 
      32827-7408, USA. Yan.liu@ucf.edu.
FAU - Gilbertson, Timothy A
AU  - Gilbertson TA
AD  - Department of Internal Medicine, University Central Florida, Orlando, FL 
      32827-7408, USA. Timothy.Gilbertson@ucf.edu.
FAU - Zhou, Anhong
AU  - Zhou A
AUID- ORCID: 0000-0001-6645-6375
AD  - Department of Biological Engineering, Utah State University, Logan, UT 
      84322-4105, USA. Anhong.Zhou@usu.edu.
LA  - eng
GR  - R01 DC013318/DC/NIDCD NIH HHS/United States
GR  - R01DC013318/NH/NIH HHS/United States
GR  - 1264498/National Science Foundation/
PT  - Journal Article
DEP - 20190408
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
PMC - PMC6480160
OTO - NOTNLM
OT  - 4-mercaptobenzoic acid
OT  - GPR120
OT  - Raman
OT  - SERS
OT  - fatty acid
OT  - microfluidics
COIS- The authors declare no conflict of interest.
EDAT- 2019/04/11 06:00
MHDA- 2019/04/11 06:01
PMCR- 2019/04/01
CRDT- 2019/04/11 06:00
PHST- 2019/02/02 00:00 [received]
PHST- 2019/03/20 00:00 [revised]
PHST- 2019/03/26 00:00 [accepted]
PHST- 2019/04/11 06:00 [entrez]
PHST- 2019/04/11 06:00 [pubmed]
PHST- 2019/04/11 06:01 [medline]
PHST- 2019/04/01 00:00 [pmc-release]
AID - s19071663 [pii]
AID - sensors-19-01663 [pii]
AID - 10.3390/s19071663 [doi]
PST - epublish
SO  - Sensors (Basel). 2019 Apr 8;19(7):1663. doi: 10.3390/s19071663.