PMID- 30966772
OWN - NLM
STAT- MEDLINE
DCOM- 20191017
LR  - 20191017
IS  - 1793-6853 (Electronic)
IS  - 0192-415X (Linking)
VI  - 47
IP  - 3
DP  - 2019
TI  - Luteolin: A Natural Flavonoid Enhances the Survival of HUVECs against Oxidative 
      Stress by Modulating AMPK/PKC Pathway.
PG  - 541-557
LID - 10.1142/S0192415X19500289 [doi]
AB  - Oxidative stress has been implicated in the pathogenesis of atherosclerotic 
      cardiovascular diseases. Dietary supplementation of anti-oxidants has been 
      reported to have beneficial effects on the prevention of atherogenic diseases. 
      Luteolin (a natural flavonoid) has been shown to possess antimutagenic, 
      antitumorigenic, anti-oxidant and anti-inflammatory properties. However, the 
      effects and underlying molecular mechanisms of luteolin on cardiovascular systems 
      are poorly explored. Therefore, the aim of the present study was to test whether 
      luteolin could protect against oxidative stress-induced endothelial cell injury 
      and explore the underlying mechanisms. In this study, human umbilical vein 
      endothelial cells (HUVECs) were pre-treated with luteolin followed by hydrogen 
      peroxide induction (H(2)O(2)). Our results showed that luteolin protected against 
      H(2)O(2)-induced oxidative stress and ameliorated ROS and superoxide generation. 
      In addition, we found that luteolin treatment inhibited the H(2)O(2)-induced 
      membrane assembly of NADPH oxidase subunits, which was further confirmed by 
      specifically inhibiting NADPH oxidase using DPI treatment. Furthermore, pAMPK 
      protein expression was enhanced and p-PKC isoforms were significantly 
      down-regulated by luteolin treatment in a dose-dependent manner, and a similar 
      effect was observed upon DPI treatment. However, co-treatment with the specific 
      inhibitor of AMPK (Compound C) restored p-PKC levels suggesting the role of AMPK 
      signaling in regulating p-PKC expression under oxidative stress condition in 
      HUVECs. Finally, we confirmed using siRNAs and specific inhibitor and/or 
      activator of AMPK (AICAR) that luteolin treatment induced AMPK is a key player 
      and regulator of activated expression of PKC isoforms and thereby confers 
      protection against H(2)O(2)-induced oxidative stress in HUVECs.
FAU - Ou, Hsiu-Chung
AU  - Ou HC
AD  - * Department of Occupational Therapy, College of Medical and Health Science, 
      Taichung, Taiwan.
FAU - Pandey, Sudhir
AU  - Pandey S
AD  - double dagger Graduate Institute of Biomedical Science, China Medical University, Taichung, 
      Taiwan.
FAU - Hung, Meng-Yu
AU  - Hung MY
AD  -  section sign Graduate Institute of Basic Medical Science, China Medical University, 
      Taichung, Taiwan.
FAU - Huang, Su-Hua
AU  - Huang SH
AD  - dagger Department of Biotechnology, Asia University, Taichung, Taiwan.
FAU - Hsu, Pei-Tz
AU  - Hsu PT
AD  - dagger Department of Biotechnology, Asia University, Taichung, Taiwan.
FAU - Day, Cecilia-Hsuan
AU  - Day CH
AD  - ** Department of Nursing, Meiho University, Pingtung, Taiwan.
FAU - Pai, Peiying
AU  - Pai P
AD  -  paragraph sign School of Medicine, College of Medicine, China Medical University, Taichung, 
      Taiwan.
AD  - daggerdagger Division of Cardiovascular Medicine, Department of Medicine, China Medical 
      University Hospital, Taichung, Taiwan.
FAU - Viswanadha, Vijaya Padma
AU  - Viswanadha VP
AD  -  section sign section sign Department of Biotechnology, Bharathiar University, Coimbatore, India.
FAU - Kuo, Wei-Wen
AU  - Kuo WW
AD  -  parallel Department of Biological Science and Technology, China Medical University, 
      Taichung, Taiwan.
FAU - Huang, Chih-Yang
AU  - Huang CY
AD  - double dagger Graduate Institute of Biomedical Science, China Medical University, Taichung, 
      Taiwan.
AD  -  section sign Graduate Institute of Basic Medical Science, China Medical University, 
      Taichung, Taiwan.
AD  - double daggerdouble dagger Medical Research Center For Exosomes and Mitochondria Related Diseases, China 
      Medical University Hospital, Taichung, Taiwan.
AD  -  paragraph sign paragraph sign Department of Clinical Laboratory, The Sixth Affiliated Hospital of Guangzhou 
      Medical University, Qingyuan People's Hospital, Guangdong, China.
AD  -  parallel parallel College of Medicine, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical 
      Foundation, Tzu Chi University, Hualien, Taiwan.
LA  - eng
PT  - Journal Article
DEP - 20190409
PL  - Singapore
TA  - Am J Chin Med
JT  - The American journal of Chinese medicine
JID - 7901431
RN  - 0 (Flavonoids)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - KUX1ZNC9J2 (Luteolin)
SB  - IM
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Cell Survival/*drug effects
MH  - Cells, Cultured
MH  - Flavonoids/*pharmacology
MH  - Human Umbilical Vein Endothelial Cells/*metabolism
MH  - Humans
MH  - Hydrogen Peroxide/adverse effects
MH  - Luteolin/*pharmacology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Oxidative Stress/*drug effects
OTO - NOTNLM
OT  - AMPK
OT  - HUVECs
OT  - Luteolin
OT  - NADPH Oxidase
OT  - PKC
OT  - ROS
EDAT- 2019/04/11 06:00
MHDA- 2019/10/18 06:00
CRDT- 2019/04/11 06:00
PHST- 2019/04/11 06:00 [pubmed]
PHST- 2019/10/18 06:00 [medline]
PHST- 2019/04/11 06:00 [entrez]
AID - 10.1142/S0192415X19500289 [doi]
PST - ppublish
SO  - Am J Chin Med. 2019;47(3):541-557. doi: 10.1142/S0192415X19500289. Epub 2019 Apr 
      9.