PMID- 30967512
OWN - NLM
STAT- MEDLINE
DCOM- 20200629
LR  - 20200629
IS  - 1937-9145 (Electronic)
IS  - 1945-0877 (Linking)
VI  - 12
IP  - 576
DP  - 2019 Apr 9
TI  - Pivotal role of STIM2, but not STIM1, in IL-4 production by IL-3-stimulated 
      murine basophils.
LID - eaav2060 [pii]
LID - 10.1126/scisignal.aav2060 [doi]
AB  - Basophils have nonredundant roles in various immune responses that require Ca(2+) 
      influx. Here, we examined the role of two Ca(2+) sensors, stromal interaction 
      molecule 1 and 2 (STIM1 and STIM2), in basophil activation. We found that loss of 
      STIM1, but not STIM2, impaired basophil IL-4 production after stimulation with 
      immunoglobulin E (IgE)-containing immune complexes. In contrast, when basophils 
      were stimulated with IL-3, loss of STIM2, but not STIM1, reduced basophil IL-4 
      production. This difference in STIM proteins was associated with distinct time 
      courses of Ca(2+) influx and transcription of the Il4 gene that were elicited by 
      each stimulus. Similarly, basophil-specific STIM1 expression was required for 
      IgE-driven chronic allergic inflammation in vivo, whereas STIM2 was required for 
      IL-4 production after combined IL-3 and IL-33 treatment in mice. These data 
      indicate that STIM1 and STIM2 have differential roles in the production of IL-4, 
      which are stimulus dependent. Furthermore, these results illustrate the vital 
      role of STIM2 in basophils, which is often considered to be less important than 
      STIM1.
CI  - Copyright (c) 2019 The Authors, some rights reserved; exclusive licensee American 
      Association for the Advancement of Science. No claim to original U.S. Government 
      Works.
FAU - Yoshikawa, Soichiro
AU  - Yoshikawa S
AUID- ORCID: 0000-0003-1820-3829
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan. 
      yoshisou.mbch@tmd.ac.jp.
FAU - Oh-Hora, Masatsugu
AU  - Oh-Hora M
AD  - Department of Biochemistry, Graduate School of Medicine, Juntendo University, 
      Tokyo 113-8421, Japan.
FAU - Hashimoto, Ryota
AU  - Hashimoto R
AUID- ORCID: 0000-0003-0983-6704
AD  - Department of Physiology, Faculty of Medicine, Juntendo University, Tokyo 
      113-8421, Japan.
FAU - Nagao, Toshihisa
AU  - Nagao T
AUID- ORCID: 0000-0003-2597-0719
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
FAU - Peters, Louis
AU  - Peters L
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
AD  - Faculty of Medicine, Imperial College London, London SW7 2BX, UK.
FAU - Egawa, Mayumi
AU  - Egawa M
AUID- ORCID: 0000-0001-5806-0039
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
FAU - Ohta, Takuya
AU  - Ohta T
AUID- ORCID: 0000-0002-4174-6349
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
FAU - Miyake, Kensuke
AU  - Miyake K
AUID- ORCID: 0000-0003-1149-3755
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
FAU - Adachi, Takahiro
AU  - Adachi T
AUID- ORCID: 0000-0002-3711-8577
AD  - Department of Immunology, Medical Research Institute, Tokyo Medical and Dental 
      University, Tokyo 113-8510, Japan.
FAU - Kawano, Yohei
AU  - Kawano Y
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
FAU - Yamanishi, Yoshinori
AU  - Yamanishi Y
AUID- ORCID: 0000-0002-9598-3542
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
FAU - Karasuyama, Hajime
AU  - Karasuyama H
AUID- ORCID: 0000-0003-0689-0836
AD  - Department of Immune Regulation, Graduate School of Medical and Dental Sciences, 
      Tokyo Medical and Dental University, Tokyo 113-8510, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190409
PL  - United States
TA  - Sci Signal
JT  - Science signaling
JID - 101465400
RN  - 0 (Il4 protein, mouse)
RN  - 0 (Interleukin-3)
RN  - 0 (Stim1 protein, mouse)
RN  - 0 (Stim2 protein, mouse)
RN  - 0 (Stromal Interaction Molecule 1)
RN  - 0 (Stromal Interaction Molecule 2)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Basophils/cytology/*immunology
MH  - Calcium Signaling/genetics/*immunology
MH  - Immunoglobulin E/immunology
MH  - Interleukin-3/genetics/*immunology
MH  - Interleukin-4/genetics/*immunology
MH  - Mice
MH  - Mice, Knockout
MH  - Stromal Interaction Molecule 1/genetics/*immunology
MH  - Stromal Interaction Molecule 2/genetics/*immunology
EDAT- 2019/04/11 06:00
MHDA- 2020/07/01 06:00
CRDT- 2019/04/11 06:00
PHST- 2019/04/11 06:00 [entrez]
PHST- 2019/04/11 06:00 [pubmed]
PHST- 2020/07/01 06:00 [medline]
AID - 12/576/eaav2060 [pii]
AID - 10.1126/scisignal.aav2060 [doi]
PST - epublish
SO  - Sci Signal. 2019 Apr 9;12(576):eaav2060. doi: 10.1126/scisignal.aav2060.