PMID- 30968846
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20200225
IS  - 1643-3750 (Electronic)
IS  - 1234-1010 (Print)
IS  - 1234-1010 (Linking)
VI  - 25
DP  - 2019 Apr 10
TI  - Serum Levels of Inflammatory Cytokines and Expression of BCL2 and BAX mRNA in 
      Peripheral Blood Mononuclear Cells and in Patients with Chronic Heart Failure.
PG  - 2633-2639
LID - 10.12659/MSM.912457 [doi]
AB  - BACKGROUND This study investigated the expression of the BCL2 and BAX mRNA, 
      inflammatory cytokines, interleukin-1ss (IL-1ss), IL-6, and tumor necrosis 
      factor-alpha (TNF-alpha), and cardiac function in patients with chronic heart 
      failure (CHF). The New York Heart Association (NYHA) Functional Classification 
      and measurement of the left ventricular ejection fraction (LVEF) evaluated 
      cardiac function. MATERIAL AND METHODS Patients with CHF (n=60) due to coronary 
      heart disease, hypertensive heart disease, and cardiomyopathy, and healthy 
      controls (n=30) were studied. Enzyme-linked immunosorbent assay (ELISA) measured 
      serum levels of IL-1ss, IL-6, and TNF-alpha. Quantitative reverse transcription 
      polymerase chain reaction (qRT-PCR) detected mRNA expression of BCL2 and BAX in 
      peripheral blood mononuclear cells (PBMCs). Color Doppler ultrasound measured the 
      LVEF, and the NYHA classification of CHF was used. RESULTS In patients with CHF, 
      levels of IL-1ss, IL-6 and TNF-alpha, and mRNA expression of BAX were 
      significantly increased compared with the control group (p<0.01); BCL2 mRNA level 
      was significantly lower (p<0.01). There were no significant differences in the 
      expression levels of inflammatory cytokines, or BCL2 or BAX mRNA in patients with 
      CHF due to coronary heart disease, hypertensive heart disease, or cardiomyopathy. 
      Expression levels of IL-1ss, IL-6, TNF-alpha, and BAX mRNA were significantly 
      associated with the degree of CHF. Cardiac function was negatively correlated 
      with LVEF (p<0.05). Expression levels of BCL2 mRNA level were negatively 
      correlated with cardiac function (p<0.05), and positively correlated with LVEF 
      (p<0.05). CONCLUSIONS Levels of IL-1ss, IL-6, TNF-alpha, and BAX mRNA were 
      negatively correlated with cardiac function, and BCL2 mRNA expression was 
      positively associated with CHF.
FAU - Liu, Wenxiu
AU  - Liu W
AD  - Department of Cardiology, Hebei Medical University, Shijiazhuang, Hebei, China 
      (mainland).
AD  - Department of Cardiology, Bethune International Peace Hospital, Shijiazhuang, 
      Hebei, China (mainland).
FAU - Ru, Leisheng
AU  - Ru L
AD  - Department of Cardiology, Bethune International Peace Hospital, Shijiazhuang, 
      Hebei, China (mainland).
FAU - Su, Chang
AU  - Su C
AD  - Department of Cardiology, Bethune International Peace Hospital, Shijiazhuang, 
      Hebei, China (mainland).
FAU - Qi, Shuying
AU  - Qi S
AD  - Department of Cardiology, Bethune International Peace Hospital, Shijiazhuang, 
      Hebei, China (mainland).
FAU - Qi, Xiaoyong
AU  - Qi X
AD  - Department of Cardiology, Hebei Medical University, Shijiazhuang, Hebei, China 
      (mainland).
LA  - eng
PT  - Journal Article
DEP - 20190410
PL  - United States
TA  - Med Sci Monit
JT  - Medical science monitor : international medical journal of experimental and 
      clinical research
JID - 9609063
RN  - 0 (BCL2 protein, human)
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Messenger)
RN  - 0 (bcl-2-Associated X Protein)
MH  - Aged
MH  - Cardiomyopathies/blood/genetics/physiopathology
MH  - Chronic Disease
MH  - Coronary Disease/blood/genetics/physiopathology
MH  - Cytokines/*blood
MH  - Female
MH  - *Gene Expression Regulation
MH  - Heart Failure/*blood/*genetics/physiopathology
MH  - Heart Function Tests
MH  - Humans
MH  - Hypertension/blood/genetics/physiopathology
MH  - Inflammation Mediators/*blood
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Male
MH  - Middle Aged
MH  - Proto-Oncogene Proteins c-bcl-2/blood/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Stroke Volume
MH  - bcl-2-Associated X Protein/blood/*genetics
PMC - PMC6474297
COIS- Conflict of interest None.
EDAT- 2019/04/11 06:00
MHDA- 2019/08/03 06:00
PMCR- 2019/04/10
CRDT- 2019/04/11 06:00
PHST- 2019/04/11 06:00 [entrez]
PHST- 2019/04/11 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
PHST- 2019/04/10 00:00 [pmc-release]
AID - 912457 [pii]
AID - 10.12659/MSM.912457 [doi]
PST - epublish
SO  - Med Sci Monit. 2019 Apr 10;25:2633-2639. doi: 10.12659/MSM.912457.