PMID- 30969151
OWN - NLM
STAT- MEDLINE
DCOM- 20190604
LR  - 20190604
IS  - 1945-0257 (Electronic)
IS  - 1945-0257 (Linking)
VI  - 23
IP  - 5
DP  - 2019 May
TI  - CCAAT/Enhancer Binding Protein beta-Mediated MMP3 Upregulation Promotes Esophageal 
      Squamous Cell Cancer Invasion In Vitro and Is Associated with Metastasis in Human 
      Patients.
PG  - 304-309
LID - 10.1089/gtmb.2018.0291 [doi]
AB  - Aims: Metastasis is a significant obstacle to curing esophageal squamous cell 
      carcinoma (ESCC). The CCAAT/enhancer binding protein beta (C/EBPbeta) and matrix 
      metalloproteinase 3 (MMP3) are thought to play key roles in cancer invasion and 
      metastasis. In this study, we aimed to detect whether C/EBPbeta-mediated tumor 
      invasion was dependent on MMP3. In addition, we determined whether C/EBPbeta 
      upregulation was associated with MMP3 levels and metastatic status in patients 
      with ESCC. Materials and Methods: A total of 126 patients with ESCC were 
      recruited for this study. The mRNA and protein levels of C/EBPbeta and MMP3 in ESCC 
      cell lines and specimens from ESCC patient were determined by reverse 
      transcription-polymerase chain reaction and western blot, respectively. Tumor 
      cell invasion was analyzed using an in vitro Matrigel Invasion Assay. The 
      correlation between C/EBPbeta and MMP3 expression was determined by Pearson's 
      correlation analysis. Results: Both mRNA and protein levels of MMP3 were 
      upregulated by C/EBPbeta overexpression and downregulated by C/EBPbeta siRNA in KYSE150 
      cell cultures. The promotion of ESCC cell invasion through C/EBPbeta was inhibited 
      by MMP3 siRNA. The level of C/EBPbeta was correlated with MMP3 and metastatic status 
      in patients with ESCC. Conclusions: C/EBPbeta upregulation promoted tumor cell 
      invasion in an MMP3-dependent manner in vitro and was associated with metastatic 
      status in ESCC.
FAU - Li, Hong
AU  - Li H
AD  - 1 Department of Oncology, Henan Cancer Hospital, Zhengzhou University, Zhengzhou, 
      Henan Province, People's Republic of China.
FAU - Yang, Fan
AU  - Yang F
AD  - 2 Department of Computer Science, University of Nebraska-Lincoln, Lincoln, 
      Nebraska.
FAU - Chai, Li
AU  - Chai L
AD  - 3 Department of Clinical Laboratory, Tongxu County People's Hospital, Tongxu, 
      Henan Province, People's Republic of China.
FAU - Zhang, Liguo
AU  - Zhang L
AD  - 4 Department of Thoracic Surgery, Xinxiang City Center Hospital, Xinxiang, Henan 
      Province, People's Republic of China.
FAU - Li, Sha
AU  - Li S
AD  - 5 Department of Molecular Laboratory, Zhengzhou Haipu Medical Laboratory, 
      Zhengzhou, People's Republic of China.
FAU - Xu, Ziguang
AU  - Xu Z
AD  - 6 Department of Pathology, Henan Provincial People's Hospital, Zhengzhou 
      University, Zhengzhou, People's Republic of China.
FAU - Kong, Lingfei
AU  - Kong L
AD  - 6 Department of Pathology, Henan Provincial People's Hospital, Zhengzhou 
      University, Zhengzhou, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20190410
PL  - United States
TA  - Genet Test Mol Biomarkers
JT  - Genetic testing and molecular biomarkers
JID - 101494210
RN  - 0 (CCAAT-Enhancer-Binding Protein-beta)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Aged
MH  - CCAAT-Enhancer-Binding Protein-beta/genetics/*physiology
MH  - Carcinoma, Squamous Cell/genetics
MH  - Cell Line, Tumor
MH  - China
MH  - Esophageal Neoplasms/genetics
MH  - Esophageal Squamous Cell Carcinoma/*genetics/physiopathology
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/genetics/metabolism/*physiology
MH  - Middle Aged
MH  - Neoplasm Invasiveness/genetics
MH  - Neoplasm Metastasis/genetics
MH  - Up-Regulation
OTO - NOTNLM
OT  - C/EBPbeta
OT  - MMP3
OT  - esophageal squamous cell carcinoma
OT  - invasion
OT  - metastasis
EDAT- 2019/04/11 06:00
MHDA- 2019/06/05 06:00
CRDT- 2019/04/11 06:00
PHST- 2019/04/11 06:00 [pubmed]
PHST- 2019/06/05 06:00 [medline]
PHST- 2019/04/11 06:00 [entrez]
AID - 10.1089/gtmb.2018.0291 [doi]
PST - ppublish
SO  - Genet Test Mol Biomarkers. 2019 May;23(5):304-309. doi: 10.1089/gtmb.2018.0291. 
      Epub 2019 Apr 10.