PMID- 30969429
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20190802
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1947
DP  - 2019
TI  - Laser Doppler Flowmetry to Study the Regulation of Cerebral Blood Flow by G 
      Protein-Coupled Receptors in Rodents.
PG  - 377-387
LID - 10.1007/978-1-4939-9121-1_22 [doi]
AB  - A large body of evidence suggests that G protein-coupled receptors (GPCRs) play 
      an important role in the regulation of peripheral vascular reactivity. Meanwhile, 
      the extent of GPCR influence on the regulation of brain vascular reactivity, or 
      cerebral blood flow (CBF), has yet to be fully appreciated. This is of 
      physiological importance as the modulation of CBF depends on an intricate 
      interplay between neurons, astrocytes, pericytes, and endothelial cells, all of 
      which partaking in the formation of a functional entity referred to as the 
      neurovascular unit (NVU). The NVU is the anatomical substrate of neurovascular 
      coupling (NVC) mechanisms, whereby increased neuronal activity leads to increased 
      blood flow to accommodate energy, oxygen, and nutrients demands. In light of 
      growing evidence showing impaired NVC in several neurological disorders, and the 
      fact that GPCRs represent the most important targets of FDA-approved drugs, it is 
      of utmost importance to use experimental approaches to study GPCR-induced 
      regulation of NVC for the future development of pharmaceutical compounds that 
      could normalize CBF function. Herein, we describe a minimally invasive approach 
      called laser Doppler flowmetry (LDF) that, when used in combination with a 
      whisker stimulation paradigm in rodents, allows gauging blood perfusion in 
      activated cerebral cortex. We comprehensively explain the surgical procedure and 
      data acquisition in mice, and discussed about important experimental 
      considerations for the study of CBF regulation by GPCRs using pharmacological 
      agents.
FAU - Toussay, Xavier
AU  - Toussay X
AD  - Ottawa Hospital Research Institute (Neurosciences), Ottawa, ON, Canada.
AD  - Department of Cellular and Molecular Medicine, University of Ottawa Brain and 
      Mind Research Institute, University of Ottawa, Ottawa, ON, Canada.
FAU - Tiberi, Mario
AU  - Tiberi M
AD  - Ottawa Hospital Research Institute (Neurosciences), Ottawa, ON, Canada.
AD  - Department of Cellular and Molecular Medicine, University of Ottawa Brain and 
      Mind Research Institute, University of Ottawa, Ottawa, ON, Canada.
FAU - Lacoste, Baptiste
AU  - Lacoste B
AD  - Ottawa Hospital Research Institute (Neurosciences), Ottawa, ON, Canada. 
      blacoste@uottawa.ca.
AD  - Department of Cellular and Molecular Medicine, University of Ottawa Brain and 
      Mind Research Institute, University of Ottawa, Ottawa, ON, Canada. 
      blacoste@uottawa.ca.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Receptors, G-Protein-Coupled)
MH  - Animals
MH  - Cerebral Cortex/*blood supply
MH  - *Cerebrovascular Circulation
MH  - Laser-Doppler Flowmetry/*methods
MH  - Mice
MH  - Rats
MH  - Receptors, G-Protein-Coupled/*metabolism
OTO - NOTNLM
OT  - Cerebral blood flow
OT  - GPCRs
OT  - Laser doppler flowmetry
OT  - Physiology
OT  - Rodents
EDAT- 2019/04/11 06:00
MHDA- 2019/08/03 06:00
CRDT- 2019/04/11 06:00
PHST- 2019/04/11 06:00 [entrez]
PHST- 2019/04/11 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
AID - 10.1007/978-1-4939-9121-1_22 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1947:377-387. doi: 10.1007/978-1-4939-9121-1_22.