PMID- 30972993
OWN - NLM
STAT- MEDLINE
DCOM- 20190618
LR  - 20190618
IS  - 0393-974X (Print)
IS  - 0393-974X (Linking)
VI  - 33
IP  - 2
DP  - 2019 Mar-Apr
TI  - Effects of exercise on sex hormones and expression of relevant genes in the 
      hypothalamus in obese mice.
PG  - 517-523
AB  - This study aimed to investigate the effect of different exercise loads on sex 
      hormones and expressions of relevant genes in hypothalamus in obese mice. Sixty 
      weaning male C57BL/6 mice were used as subjects. Among them, 15 mice were 
      randomly classified into the normal diet group (CON group), and the remaining 45 
      mice into high-fat diet group (MOB group). The obesity was successfully achieved 
      by high-fat diet 10 weeks later. Then the rats were randomly divided into three 
      groups based on weight, namely, obesity control group (OBC group), obesity with 
      moderate-intensity exercise group (MOBC group), and obesity with high-intensity 
      exercise group (HOBC group), with 15 mice in each group. Mice in the MOBC group 
      and HOBC group were offered 8 weeks of swimming training, and the exercise time 
      increased incrementally until 2 h and 4 h per day. After the training was over, 
      ELISA method was used to determine the serum levels of Adiponectin (Adipo), 
      luteotropic hormone (LH), follicle-stimulating hormone (FSH) and testosterone 
      (T). Real-time PCR was implemented to detect the transcriptional levels of genes 
      of Adipo and other relevant proteins in the hypothalamus. The result showed that 
      compared with the CON group, there was a significant reduction in the serum 
      levels of Adipo, LH, FSH and T in the OBC group (P<0.05). As compared with the 
      OBC group, the serum levels of Adipo, LH, FSH and T increased significantly in 
      the OBC group (P<0.05). There was a significant increase in the transcriptional 
      levels of Adipo, Adipo receptor 1 (Adipo R1), and AMP-activated protein kinase 
      (AMPK) in the OBC group (P<0.05) compared to in the CON group; meanwhile, the 
      transcriptional levels of kisspeptin (Kiss) and gonadotropin-releasing hormone 
      (GnRH) decreased significantly (P<0.05). In conclusion, long-term 
      moderate-intensity exercise could improve the negative effect of obesity on sex 
      development. Long-term high-intensity exercises could not improve the inhibitory 
      effect of obesity on sex development.
FAU - Wang, J M
AU  - Wang JM
AD  - School of Sports Science, Nantong University, Nantong, Jiangsu, China.
FAU - Gong, T Y
AU  - Gong TY
AD  - School of Sports Science, Nantong University, Nantong, Jiangsu, China.
FAU - Zhao, Z J
AU  - Zhao ZJ
AD  - School of Sports Science, Zhengzhou University, Zhengzhou, Henan, China.
LA  - eng
PT  - Letter
PL  - Italy
TA  - J Biol Regul Homeost Agents
JT  - Journal of biological regulators and homeostatic agents
JID - 8809253
RN  - 0 (Adiponectin)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Kisspeptins)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 3XMK78S47O (Testosterone)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - EC 2.7.4.3 (Adenylate Kinase)
SB  - IM
MH  - Adenylate Kinase/metabolism
MH  - Adiponectin/blood
MH  - Animals
MH  - Follicle Stimulating Hormone/blood
MH  - Gonadal Steroid Hormones/*blood
MH  - Gonadotropin-Releasing Hormone/metabolism
MH  - Hypothalamus/*metabolism
MH  - Kisspeptins/metabolism
MH  - Luteinizing Hormone/blood
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Obese
MH  - Obesity/*genetics
MH  - *Physical Conditioning, Animal
MH  - Random Allocation
MH  - Testosterone/blood
OTO - NOTNLM
OT  - Adiponectin
OT  - HPG axis
OT  - endocrine
OT  - exercise intervention
OT  - obesity
EDAT- 2019/04/12 06:00
MHDA- 2019/06/19 06:00
CRDT- 2019/04/12 06:00
PHST- 2019/04/12 06:00 [entrez]
PHST- 2019/04/12 06:00 [pubmed]
PHST- 2019/06/19 06:00 [medline]
AID - 31 [pii]
PST - ppublish
SO  - J Biol Regul Homeost Agents. 2019 Mar-Apr;33(2):517-523.