PMID- 30974174
OWN - NLM
STAT- MEDLINE
DCOM- 20200110
LR  - 20200110
IS  - 1879-0240 (Electronic)
IS  - 0965-1748 (Linking)
VI  - 109
DP  - 2019 Jun
TI  - Insect hemolymph coagulation: Kinetics of classically and non-classically 
      secreted clotting factors.
PG  - 63-71
LID - S0965-1748(18)30441-7 [pii]
LID - 10.1016/j.ibmb.2019.04.007 [doi]
AB  - In most insects, hemolymph coagulation, which is analogous to mammalian blood 
      clotting, involves close collaboration between humoral and cellular components. 
      To gain insights into the secretion of cellular clotting factors, we created 
      tagged versions of three different clotting factors. Our focus was on factors 
      which are released in a non-classical manner and to characterize them in 
      comparison to a protein that is classically released, namely Glutactin (Glt). 
      Transglutaminase-A (Tg) and Prophenoloxidase 2 (PPO2), both of which lack signal 
      peptide sequences, have been previously demonstrated to be released from 
      plasmatocytes and crystal cells (CCs) respectively, the two hemocyte classes in 
      naive larvae. We found that at the molecular level, Tg secretion resembles the 
      release of tissue transglutaminase in mammals. Specifically, Drosophila Tg is 
      associated with vesicular membranes and remains membrane-bound after release, in 
      contrast to Glt, which we found localizes to a different class of vesicles and is 
      integrated into clot fibers. PPO2 on the other hand, is set free from CCs through 
      cytolysis. We confirm that PPO2 is a central component of the cytosolic crystals 
      and find that the distribution of PPO2 appears to vary across crystals and cells. 
      We propose a tentative scheme for the secretory events during early and late 
      hemolymph coagulation.
CI  - Copyright (c) 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Schmid, Martin R
AU  - Schmid MR
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: martin.schmid@su.se.
FAU - Dziedziech, Alexis
AU  - Dziedziech A
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: 
      alexis.dziedziech@su.se.
FAU - Arefin, Badrul
AU  - Arefin B
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: 
      badrul.arefin@liu.se.
FAU - Kienzle, Thomas
AU  - Kienzle T
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: thomas-k1@gmx.net.
FAU - Wang, Zhi
AU  - Wang Z
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: zhiwang33@gmail.com.
FAU - Akhter, Munira
AU  - Akhter M
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: munira.akhter@su.se.
FAU - Berka, Jakub
AU  - Berka J
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: 
      berkajakub@seznam.cz.
FAU - Theopold, Ulrich
AU  - Theopold U
AD  - Stockholm University, Department of Molecular Biosciences, The Wenner-Gren 
      Institute, SE-106 91, Stockholm, Sweden. Electronic address: uli.theopold@su.se.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190408
PL  - England
TA  - Insect Biochem Mol Biol
JT  - Insect biochemistry and molecular biology
JID - 9207282
RN  - 0 (Blood Coagulation Factors)
RN  - 0 (Drosophila Proteins)
SB  - IM
MH  - Animals
MH  - Blood Coagulation Factors/*genetics/metabolism
MH  - Drosophila Proteins/genetics/*physiology
MH  - Drosophila melanogaster/genetics/*physiology
MH  - Hemolymph/*physiology
OTO - NOTNLM
OT  - Bacteria
OT  - Clotting
OT  - Coagulation
OT  - Defense
OT  - Drosophila melanogaster
OT  - Glutactin
OT  - Hemolymph
OT  - Non-classical secretion
OT  - Prophenoloxidase
OT  - Secretion
OT  - Septic injury
OT  - Transglutaminase
OT  - Wounding
EDAT- 2019/04/12 06:00
MHDA- 2020/01/11 06:00
CRDT- 2019/04/12 06:00
PHST- 2018/11/29 00:00 [received]
PHST- 2019/03/27 00:00 [revised]
PHST- 2019/04/02 00:00 [accepted]
PHST- 2019/04/12 06:00 [pubmed]
PHST- 2020/01/11 06:00 [medline]
PHST- 2019/04/12 06:00 [entrez]
AID - S0965-1748(18)30441-7 [pii]
AID - 10.1016/j.ibmb.2019.04.007 [doi]
PST - ppublish
SO  - Insect Biochem Mol Biol. 2019 Jun;109:63-71. doi: 10.1016/j.ibmb.2019.04.007. 
      Epub 2019 Apr 8.