PMID- 30974362
OWN - NLM
STAT- MEDLINE
DCOM- 20190621
LR  - 20190621
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 672
DP  - 2019 Jul 1
TI  - Microbial induced carbonate precipitation for immobilizing Pb contaminants: Toxic 
      effects on bacterial activity and immobilization efficiency.
PG  - 722-731
LID - S0048-9697(19)31286-0 [pii]
LID - 10.1016/j.scitotenv.2019.03.294 [doi]
AB  - Microbial induced carbonate precipitation (MICP) is a natural bio-mediated 
      process, which has been explored for soil stabilization and heavy metals 
      immobilization in soil and groundwater. Previous studies have shown that MICP is 
      capable of immobilizing various heavy metals including lead (Pb). However, most 
      studies focus merely on the immobilization of heavy metals with relatively low 
      concentration. This study: (1) presents results of an investigation into the 
      toxic effects of Pb on bacterial activity and immobilization efficiency within a 
      wide range of Pb concentrations; and (2) identifies controlling biotic and 
      abiotic factors of Pb immobilization by MICP. In the first series of tests, 
      bacterial strains (Sporosarcina pasteurii) are inoculated into nutrient solutions 
      containing 0-50 mM Pb(NO(3))(2) and incubated at 30  degrees C. Biochemical parameters 
      are measured over time, which include pH, electrical conductivity, urease 
      activity, and viable cell number. In the second series of tests, grown bacterial 
      strains are mixed with urea, calcium salts and Pb(NO(3))(2) in solution. Viable 
      cell number, produced ammonium concentration, aqueous Pb concentration of the 
      mixed solution, and total precipitation mass are measured. The results show that 
      the presence of Pb has marginal effect on bacterial growth and associated urease 
      activity at Pb concentration < 30 mM. The calcium source and initial bacteria 
      concentration are found to remarkably influence Pb immobilization efficiency in 
      terms of Pb removal percentage. Supplementary geochemical simulation results 
      indicate that the Pb immobilization mechanisms includes abiotic precipitation, 
      biotic precipitation and bio-sorption.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Jiang, Ning-Jun
AU  - Jiang NJ
AD  - Jiangsu Key Laboratory of Urban Underground Engineering & Environmental Safety, 
      Institute of Geotechnical Engineering, Southeast University, Nanjing 210096, 
      China; Department of Civil and Environmental Engineering, University of Hawaii at 
      Manoa, Honolulu, HI 96822, USA. Electronic address: jiangn@hawaii.edu.
FAU - Liu, Rui
AU  - Liu R
AD  - Jiangsu Key Laboratory of Urban Underground Engineering & Environmental Safety, 
      Institute of Geotechnical Engineering, Southeast University, Nanjing 210096, 
      China. Electronic address: liurui1926@seu.edu.cn.
FAU - Du, Yan-Jun
AU  - Du YJ
AD  - Jiangsu Key Laboratory of Urban Underground Engineering & Environmental Safety, 
      Institute of Geotechnical Engineering, Southeast University, Nanjing 210096, 
      China. Electronic address: duyanjun@seu.edu.cn.
FAU - Bi, Yu-Zhang
AU  - Bi YZ
AD  - Jiangsu Key Laboratory of Urban Underground Engineering & Environmental Safety, 
      Institute of Geotechnical Engineering, Southeast University, Nanjing 210096, 
      China. Electronic address: biyuzhang@imde.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20190320
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Metals, Heavy)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 2P299V784P (Lead)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacteria/*drug effects/metabolism
MH  - Biodegradation, Environmental/drug effects
MH  - Calcium Carbonate/metabolism
MH  - Groundwater
MH  - Lead/*toxicity
MH  - Metals, Heavy
MH  - Soil
MH  - *Soil Microbiology
MH  - Soil Pollutants/*toxicity
OTO - NOTNLM
OT  - Lead contamination
OT  - Microbial induced carbonate precipitation
OT  - Sporosarcina pasteurii
OT  - Urease activity
EDAT- 2019/04/12 06:00
MHDA- 2019/06/22 06:00
CRDT- 2019/04/12 06:00
PHST- 2018/10/22 00:00 [received]
PHST- 2019/03/14 00:00 [revised]
PHST- 2019/03/19 00:00 [accepted]
PHST- 2019/04/12 06:00 [pubmed]
PHST- 2019/06/22 06:00 [medline]
PHST- 2019/04/12 06:00 [entrez]
AID - S0048-9697(19)31286-0 [pii]
AID - 10.1016/j.scitotenv.2019.03.294 [doi]
PST - ppublish
SO  - Sci Total Environ. 2019 Jul 1;672:722-731. doi: 10.1016/j.scitotenv.2019.03.294. 
      Epub 2019 Mar 20.