PMID- 30974676
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231006
IS  - 2073-4360 (Electronic)
IS  - 2073-4360 (Linking)
VI  - 8
IP  - 11
DP  - 2016 Nov 16
TI  - Patterned Fibers Embedded Microfluidic Chips Based on PLA and PDMS for Ag 
      Nanoparticle Safety Testing.
LID - 10.3390/polym8110402 [doi]
LID - 402
AB  - A new method to integrate poly-dl-lactide (PLA) patterned electrospun fibers with 
      a polydimethylsiloxane (PDMS) microfluidic chip was successfully developed via 
      lithography. Hepatocyte behavior under static and dynamic conditions was 
      investigated. Immunohistochemical analyses indicated good hepatocyte survival 
      under the dynamic culture system with effective hepatocyte spheroid formation in 
      the patterned microfluidic chip vs. static culture conditions and tissue culture 
      plate (TCP). In particular, hepatocytes seeded in this microfluidic chip under a 
      flow rate of 10 muL/min could re-establish hepatocyte polarity to support biliary 
      excretion and were able to maintain high levels of albumin and urea secretion 
      over 15 days. Furthermore, the optimized system could produce sensitive and 
      consistent responses to nano-Ag-induced hepatotoxicity during culture. Thus, this 
      microfluidic chip device provides a new means of fabricating complex liver 
      tissue-engineered scaffolds, and may be of considerable utility in the toxicity 
      screening of nanoparticles.
FAU - Liu, Yaowen
AU  - Liu Y
AD  - College of Food Science, Sichuan Agricultural University, Yaan 625014, China. 
      lyw@my.swjtu.edu.cn.
AD  - School of Materials Science and Engineering, Southwest Jiaotong University, 
      Chengdu 610031, China. lyw@my.swjtu.edu.cn.
FAU - Wang, Shuyao
AU  - Wang S
AD  - College of Food Science, Sichuan Agricultural University, Yaan 625014, China. 
      shuyaow@163.com.
FAU - Wang, Yihao
AU  - Wang Y
AD  - College of Food Science, Sichuan Agricultural University, Yaan 625014, China. 
      cdyihaow@163.com.
LA  - eng
PT  - Journal Article
DEP - 20161116
PL  - Switzerland
TA  - Polymers (Basel)
JT  - Polymers
JID - 101545357
PMC - PMC6431932
OTO - NOTNLM
OT  - hepatocyte spheroid
OT  - microfluidic chips
OT  - nano-toxicity
OT  - patterned fibers
COIS- The authors declare no conflict of interest.
EDAT- 2016/11/16 00:00
MHDA- 2016/11/16 00:01
PMCR- 2016/11/16
CRDT- 2019/04/13 06:00
PHST- 2016/09/30 00:00 [received]
PHST- 2016/11/08 00:00 [revised]
PHST- 2016/11/10 00:00 [accepted]
PHST- 2019/04/13 06:00 [entrez]
PHST- 2016/11/16 00:00 [pubmed]
PHST- 2016/11/16 00:01 [medline]
PHST- 2016/11/16 00:00 [pmc-release]
AID - polym8110402 [pii]
AID - polymers-08-00402 [pii]
AID - 10.3390/polym8110402 [doi]
PST - epublish
SO  - Polymers (Basel). 2016 Nov 16;8(11):402. doi: 10.3390/polym8110402.