PMID- 30977342
OWN - NLM
STAT- MEDLINE
DCOM- 20190731
LR  - 20190731
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 4
DP  - 2019 Apr 8
TI  - [Asymmetrical flow field flow fractionation combined with ultra-performance 
      liquid chromatography-quadrupole time-of-flight mass spectrometry used to screen 
      allergen protein epitopes].
PG  - 398-403
LID - 10.3724/SP.J.1123.2018.11047 [doi]
AB  - Asymmetrical flow field flow fractionation (AF4) combined with ultra-performance 
      liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) 
      was used to screen allergen protein epitopes. The selected allergen protein 
      (tropomyosin, TM) was enzymatically digested into peptide segments and analyzed 
      via UPLC-QTOF-MS to establish a protein-specific peptide database. The peptide 
      segments were incubated with immunoglobulin E (IgE) for 30 min. During the 
      incubation procedure, the specific peptide segments (with the antigen epitope) 
      combine with IgE while the other peptide segments remain in solution. After 
      incubation, the solution was injected into the AF4 device. The combined peptide 
      segments flowed out of the outlet along with IgE, and the other peptide segments 
      flowed into the waste liquid. The components of outlet were then collected, 
      analyzed by UPLC-QTOF-MS, and the results matched with the spectra of the protein 
      peptides. Eventually the specific peptide segments were identified to detect the 
      antigen epitopes. This study extends the application of AF4 with a preliminary 
      exploration of the detection of an allergen protein epitope, providing a novel 
      research strategy for the screening of allergen epitopes.
FAU - Li, Yang
AU  - Li Y
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
AD  - Beijing Center for Disease Prevention and Control, Beijing 100013, China.
FAU - Yang, Yi
AU  - Yang Y
AD  - Beijing Center for Disease Prevention and Control, Beijing 100013, China.
FAU - Shao, Bing
AU  - Shao B
AD  - Beijing Center for Disease Prevention and Control, Beijing 100013, China.
FAU - Zou, Yue
AU  - Zou Y
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Song, Yu
AU  - Song Y
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Shu, Lin
AU  - Shu L
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Liang, Qihui
AU  - Liang Q
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
FAU - Han, Nanyin
AU  - Han N
AD  - School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Allergens)
RN  - 0 (Epitopes)
RN  - 0 (Proteins)
RN  - 0 (Tropomyosin)
MH  - Allergens/*analysis
MH  - Chromatography, High Pressure Liquid
MH  - Epitopes/*analysis
MH  - *Fractionation, Field Flow
MH  - Proteins/*analysis
MH  - Tandem Mass Spectrometry
MH  - Tropomyosin/analysis
OTO - NOTNLM
OT  - allergen
OT  - asymmetrical flow field flow fractionation (AF4)
OT  - epitope
OT  - immunoglobulin E (IgE)
OT  - mass spectrometry (MS)
EDAT- 2019/04/13 06:00
MHDA- 2019/08/01 06:00
CRDT- 2019/04/13 06:00
PHST- 2019/04/13 06:00 [entrez]
PHST- 2019/04/13 06:00 [pubmed]
PHST- 2019/08/01 06:00 [medline]
AID - 10.3724/SP.J.1123.2018.11047 [doi]
PST - ppublish
SO  - Se Pu. 2019 Apr 8;37(4):398-403. doi: 10.3724/SP.J.1123.2018.11047.