PMID- 30977425
OWN - NLM
STAT- MEDLINE
DCOM- 20190802
LR  - 20190802
IS  - 2169-141X (Electronic)
IS  - 2169-1401 (Linking)
VI  - 47
IP  - 1
DP  - 2019 Dec
TI  - The therapeutic effect of secretome from human umbilical cord-derived mesenchymal 
      stem cells in age-related osteoporosis.
PG  - 1357-1366
LID - 10.1080/21691401.2019.1596945 [doi]
AB  - Senile osteoporosis is closely related to the loss of function of stem cells. In 
      this study, we tried to investigate the potential of secretome from human 
      umbilical cord-derived mesenchymal stem cells (hUCMSCs) in recovering stem cell 
      ability from senescence and then delaying bone loss. We first harvested bone 
      marrow-derived mesenchymal stem cells (BMSCs) from young and old rats and then 
      compared their cellular characteristics such as cell growth, anti-senescence and 
      differentiation. The results showed that these abilities were negatively affected 
      by animal aging. Subsequently, aged BMSCs were exposed to secretome from hUCMSCs, 
      and we found that this loss of cell potential can be modified by secretome 
      treatment. Thereafter, the secretome was loaded into silk fibroin-based hydrogels 
      and used for an in vivo animal study. The results showed that compared to the old 
      untreated group, the bone formation capacity of aged rats was improved by local 
      treatment of secretome-loaded silk fibroin hydrogels. In conclusion, these 
      findings demonstrated that secretome from hUCMSCs has the capacity to recover 
      stem cell potential and delay local bone loss in age-related osteoporosis, which 
      could potentially be applied in osteoporosis therapy in the future.
FAU - Liang, Min
AU  - Liang M
AD  - a Department of Spine Surgery , the First Affiliated Hospital of Harbin Medical 
      University , Harbin , China.
FAU - Liu, Wanguo
AU  - Liu W
AD  - b Department of Orthopaedic Surgery, China-Japan Union Hospital , Jilin 
      University , Changchun , China.
FAU - Peng, Zhibin
AU  - Peng Z
AD  - a Department of Spine Surgery , the First Affiliated Hospital of Harbin Medical 
      University , Harbin , China.
FAU - Lv, Shihong
AU  - Lv S
AD  - c Department of Gastroenterol , the Second Affiliated Hospital of Mudanjiang 
      Medical University , Mudanjiang , China.
FAU - Guan, Ying
AU  - Guan Y
AD  - a Department of Spine Surgery , the First Affiliated Hospital of Harbin Medical 
      University , Harbin , China.
FAU - An, Gang
AU  - An G
AD  - a Department of Spine Surgery , the First Affiliated Hospital of Harbin Medical 
      University , Harbin , China.
FAU - Zhang, Yubo
AU  - Zhang Y
AD  - a Department of Spine Surgery , the First Affiliated Hospital of Harbin Medical 
      University , Harbin , China.
FAU - Huang, Tianwen
AU  - Huang T
AD  - a Department of Spine Surgery , the First Affiliated Hospital of Harbin Medical 
      University , Harbin , China.
FAU - Wang, Yansong
AU  - Wang Y
AD  - a Department of Spine Surgery , the First Affiliated Hospital of Harbin Medical 
      University , Harbin , China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Artif Cells Nanomed Biotechnol
JT  - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN  - 0 (Reactive Oxygen Species)
MH  - Animals
MH  - Biomechanical Phenomena
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Humans
MH  - Intracellular Space/metabolism
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/*cytology
MH  - Osteoporosis/pathology/*therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reactive Oxygen Species/metabolism
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - Osteoporosis
OT  - delay bone loss
OT  - hydrogel
OT  - secretome
EDAT- 2019/04/13 06:00
MHDA- 2019/08/03 06:00
CRDT- 2019/04/13 06:00
PHST- 2019/04/13 06:00 [entrez]
PHST- 2019/04/13 06:00 [pubmed]
PHST- 2019/08/03 06:00 [medline]
AID - 10.1080/21691401.2019.1596945 [doi]
PST - ppublish
SO  - Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1357-1366. doi: 
      10.1080/21691401.2019.1596945.