PMID- 30977826
OWN - NLM
STAT- MEDLINE
DCOM- 20200518
LR  - 20200518
IS  - 1758-4469 (Electronic)
IS  - 0829-318X (Linking)
VI  - 39
IP  - 7
DP  - 2019 Jul 18
TI  - Copper accumulation, subcellular partitioning and physiological and molecular 
      responses in relation to different copper tolerance in apple rootstocks.
PG  - 1215-1234
LID - 10.1093/treephys/tpz042 [doi]
AB  - To unravel the physiological and molecular regulation mechanisms underlying the 
      variation in copper (Cu)accumulation, translocation and tolerance among five 
      apple rootstocks, seedlings were exposed to either basal or excess Cu. Excess Cu 
      suppressed plant biomass and root architecture, which was less pronounced in 
      Malus prunifolia Borkh., indicating its relatively higher Cu tolerance. Among the 
      five apple rootstocks, M. prunifolia exhibited the highest Cu concentration and 
      bio-concentration factor in roots but the lowest translocation factor, indicating 
      its greater ability to immobilize Cu and restrict translocation to the aerial 
      parts. Higher Cu concentration in cell wall fraction but lower Cu proportion in 
      membrane-containing and organelle-rich fractions were found in M. prunifolia. 
      Compared with the other four apple rootstocks under excess Cu conditions, M. 
      prunifolia had a lower increment of hydrogen peroxide in roots and leaves and 
      malondialdehyde in roots, but higher concentrations of carbohydrates and enhanced 
      antioxidants. Transcript levels of genes involved in Cu uptake, transport and 
      detoxification revealed species-specific differences that are probably related to 
      alterations in Cu tolerance. M. prunifolia had relatively higher gene transcript 
      levels including copper transporters 2 (COPT2), COPT6 and zinc/iron-regulated 
      transporter-related protein 2 (ZIP2), which probably took part in Cu uptake, and 
      C-type ATP-binding cassette transporter 2 (ABCC2), copper chaperone for Cu/Zn 
      superoxide dismutase (CCS), Cu/Zn superoxide dismutase 1 (CSD1) and 
      metallothionein 2 (MT2) probably implicated in Cu detoxification, and relatively 
      lower mRNA levels of yellow stripe-like transporter 3 (YSL3) and heavy metal 
      ATPase 5 (HMA5) involved in transport of Cu to aerial parts. These results 
      suggest that M. prunifolia is more tolerant to excess Cu than the other four 
      apple rootstocks under the current experimental conditions, which is probably 
      attributed to more Cu retention in roots, subcellular partitioning, 
      well-coordinated antioxidant defense mechanisms and transcriptional expression of 
      genes involved in Cu uptake, translocation and detoxification.
CI  - (c) The Author(s) 2019. Published by Oxford University Press. All rights reserved. 
      For permissions, please e-mail: journals.permissions@oup.com.
FAU - Wan, Huixue
AU  - Wan H
AD  - College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, 
      People's Republic of China.
AD  - Key Lab of Fruit Quality Development and Regulation of Liaoning Province, 
      Shenyang, Liaoning, People's Republic of China.
FAU - Du, Jiayi
AU  - Du J
AD  - College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, 
      People's Republic of China.
AD  - Key Lab of Fruit Quality Development and Regulation of Liaoning Province, 
      Shenyang, Liaoning, People's Republic of China.
FAU - He, Jiali
AU  - He J
AD  - College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, 
      People's Republic of China.
AD  - Key Lab of Fruit Quality Development and Regulation of Liaoning Province, 
      Shenyang, Liaoning, People's Republic of China.
FAU - Lyu, Deguo
AU  - Lyu D
AD  - College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning, 
      People's Republic of China.
AD  - Key Lab of Fruit Quality Development and Regulation of Liaoning Province, 
      Shenyang, Liaoning, People's Republic of China.
FAU - Li, Huifeng
AU  - Li H
AD  - Institute of Pomology, Shandong Academy of Agricultural Sciences, Tai'an, 
      People's Republic of China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Tree Physiol
JT  - Tree physiology
JID - 100955338
RN  - 789U1901C5 (Copper)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Copper
MH  - *Malus
MH  - Plant Leaves
MH  - Plant Roots
MH  - Superoxide Dismutase
OTO - NOTNLM
OT  - Malus rootstock
OT  - copper (Cu)
OT  - gene expression
OT  - subcellular distribution
OT  - tolerance
EDAT- 2019/04/13 06:00
MHDA- 2020/05/19 06:00
CRDT- 2019/04/13 06:00
PHST- 2019/02/02 00:00 [received]
PHST- 2019/04/01 00:00 [accepted]
PHST- 2019/04/13 06:00 [pubmed]
PHST- 2020/05/19 06:00 [medline]
PHST- 2019/04/13 06:00 [entrez]
AID - 5445898 [pii]
AID - 10.1093/treephys/tpz042 [doi]
PST - ppublish
SO  - Tree Physiol. 2019 Jul 18;39(7):1215-1234. doi: 10.1093/treephys/tpz042.