PMID- 30978447
OWN - NLM
STAT- MEDLINE
DCOM- 20190716
LR  - 20190716
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 89
DP  - 2019 Jun
TI  - Production and functional insights into the potential regulation of three 
      isoforms of grass carp p40 subunit in inflammation.
PG  - 403-410
LID - S1050-4648(19)30253-0 [pii]
LID - 10.1016/j.fsi.2019.04.015 [doi]
AB  - The p40 subunit is known as a component of Interleukin (IL)-12 and IL-23. In 
      mammals, p40 can be secreted as a monomer or homodimer and acts independently to 
      mediate cellular responses. Recently, three p40 paralogues were isolated and 
      identified from grass carp and other fish species, but whether they exist 
      independently as well as their functional consequences and significance remain 
      unclear. In the present study, using grass carp as the model, we for the first 
      time demonstrated the existence of natural fish p40a, p40b and p40c (gcp40a, 
      gcp40b and gcp40c) mainly as a monomer in culture supernatant of head kidney 
      leukocytes (HKLs). Particularly, their excessive secretion induced by various 
      immune stimuli suggests possible involvement of free p40s in fish immune 
      responses. To define their functions, recombinant grass carp p40a/b/c (rgcp40a, 
      rgcp40b and rgcp40c) were prepared by Pichia pastoris expression system, and they 
      possessed the activities to enhance the secretion of pro-inflammatory cytokines 
      including Il-1beta and tumor necrosis factor-alpha (Tnf-alpha) in grass carp HKLs. These 
      pro-inflammatory properties of p40 isoforms prompted us to investigate their 
      roles during the inflammatory process. In line with this, in vivo study revealed 
      the pathogenic effect of rgcp40a on intestinal inflammation, whereas gcp40a 
      polyclonal antibodies remarkably ameliorated Aeromonas hydrophila-induced 
      intestinal histopathological changes. Taken together, our results uncover the 
      biological significance of free p40s in teleost, and provide new clue for 
      targeting fish intestinal inflammation.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Feng, Shiyu
AU  - Feng S
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Qiu, Xingyang
AU  - Qiu X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wang, Yanyan
AU  - Wang Y
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Zhang, Na
AU  - Zhang N
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Liao, Bohua
AU  - Liao B
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Zhang, Anying
AU  - Zhang A
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Yang, Kun
AU  - Yang K
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China. Electronic address: 
      zhouhongzh@uestc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190410
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Protein Isoforms)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Aeromonas hydrophila/physiology
MH  - Animals
MH  - Carps/*immunology
MH  - Fish Diseases/*immunology
MH  - Fish Proteins/*immunology
MH  - Gene Expression Profiling/veterinary
MH  - Gene Expression Regulation/immunology
MH  - Gram-Negative Bacterial Infections/immunology/veterinary
MH  - Inflammation/immunology/veterinary
MH  - Interleukin-12 Subunit p40/*immunology
MH  - Protein Isoforms/immunology
MH  - Recombinant Proteins/immunology
OTO - NOTNLM
OT  - Grass carp
OT  - IL12/IL23p40 subunit
OT  - Independent secretion
OT  - Intestinal inflammation
OT  - Isoforms
OT  - Paralogue
OT  - Pro-inflammatory activity
EDAT- 2019/04/13 06:00
MHDA- 2019/07/17 06:00
CRDT- 2019/04/13 06:00
PHST- 2018/12/18 00:00 [received]
PHST- 2019/03/28 00:00 [revised]
PHST- 2019/04/05 00:00 [accepted]
PHST- 2019/04/13 06:00 [pubmed]
PHST- 2019/07/17 06:00 [medline]
PHST- 2019/04/13 06:00 [entrez]
AID - S1050-4648(19)30253-0 [pii]
AID - 10.1016/j.fsi.2019.04.015 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Jun;89:403-410. doi: 10.1016/j.fsi.2019.04.015. Epub 
      2019 Apr 10.