PMID- 30978448
OWN - NLM
STAT- MEDLINE
DCOM- 20190716
LR  - 20190716
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 89
DP  - 2019 Jun
TI  - Effect of CXCL12-expressing viral hemorrhagic septicemia virus replicon particles 
      on leukocytes migration and vaccine efficacy in olive flounder (Paralichthys 
      olivaceus).
PG  - 378-383
LID - S1050-4648(19)30256-6 [pii]
LID - 10.1016/j.fsi.2019.04.018 [doi]
AB  - Viral replicon particles are single-cycle viruses defective for function(s) 
      needed for viral replication, which allow them to be recognized as a safer form 
      for the vaccination of animals compared to attenuated live viruses. However, 
      deletion of genes that are critical for the induction of protective immunity can 
      diminish the vaccine potential of viral replicon particles. Therefore, the 
      manipulation of viral replicon particles to produce a molecular adjuvant can be a 
      way to increase immunogenicity of vaccines based on viral replicon particles. 
      Chemokines are a class of chemotactic cytokines that control the migration of 
      diverse cells of vertebrates. CXC chemokine ligand 12 (CXCL12) binds to a 
      receptor CXCR4, and CXCL12-CXCR4 signaling plays an important role in the 
      migration of hematopoietic cells during embryogenesis and the attraction of 
      leukocytes. In the present study, to evaluate the possible use of CXCL12 as a 
      molecular adjuvant for an rVHSV-DeltaG vaccine and to know differences between 
      CXCL12a and CXCL12b in the adjuvant ability, we rescued VHSV replicon particles 
      that are expressing olive flounder CXCL12a, CXCL12b, or eGFP (rVHSV-DeltaG-CXCL12a, 
      rVHSV-DeltaG-CXCL12b, or rVHSV-DeltaG-eGFP), and compared the ability to attract olive 
      flounder leucocytes and to induce protection against a VHSV challenge. In the 
      leukocytes migration assay, supernatants collected from cells infected with 
      rVHSV-DeltaG-CXCL12a and rVHSV-DeltaG-CXCL12b showed significantly higher ability to 
      attract olive flounder leukocytes than the supernatant of cells infected with 
      rVHSV-DeltaG-eGFP. Moreover, the significantly higher number of leukocytes were 
      attracted to rVHSV-CXCL12a supernatant compared to rVHSV-CXCL12b supernatant, 
      suggesting that CXCL12a would be more appropriate for the induction of immunity 
      than CXCL12b in olive flounder. In the immunization experiment, olive flounder 
      immunized with rVHSV-DeltaG-CXCL12a showed significantly higher survival rate than 
      fish immunized with rVHSV-DeltaG-CXCL12b or rVHSV-DeltaG-eGFP. In addition, fish 
      immunized with rVHSV-DeltaG-CXCL12a showed the highest serum neutralization activity. 
      These results suggest the availability of CXCL12a for a molecular adjuvant of 
      vaccines based on VHSV replicon particles.
CI  - Copyright (c) 2019 Elsevier Ltd. All rights reserved.
FAU - Choi, Myoung Gwang
AU  - Choi MG
AD  - Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, 
      South Korea.
FAU - Kim, Min Sun
AU  - Kim MS
AD  - Department of Integrative Bio-industrial Engineering, Sejong University, Seoul, 
      05006, South Korea.
FAU - Choi, Tae-Jin
AU  - Choi TJ
AD  - Department of Microbiology, Pukyong National University, Busan, 48513, South 
      Korea.
FAU - Kim, Ki Hong
AU  - Kim KH
AD  - Department of Aquatic Life Medicine, Pukyong National University, Busan, 48513, 
      South Korea. Electronic address: khkim@pknu.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20190409
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Fish Proteins)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Cell Movement
MH  - Chemokine CXCL12/*immunology
MH  - Fish Diseases/immunology/*prevention & control/virology
MH  - Fish Proteins/*immunology
MH  - Flatfishes/*immunology
MH  - Hemorrhagic Septicemia, Viral/immunology/*prevention & control/virology
MH  - Leukocytes/immunology/physiology
MH  - Novirhabdovirus/*immunology
MH  - Random Allocation
MH  - Replicon/immunology
MH  - Viral Vaccines/*administration & dosage/immunology
OTO - NOTNLM
OT  - CXCL12a
OT  - Molecular adjuvant
OT  - VHSV replicon particle
OT  - Vaccine
EDAT- 2019/04/13 06:00
MHDA- 2019/07/17 06:00
CRDT- 2019/04/13 06:00
PHST- 2018/11/20 00:00 [received]
PHST- 2019/03/26 00:00 [revised]
PHST- 2019/04/05 00:00 [accepted]
PHST- 2019/04/13 06:00 [pubmed]
PHST- 2019/07/17 06:00 [medline]
PHST- 2019/04/13 06:00 [entrez]
AID - S1050-4648(19)30256-6 [pii]
AID - 10.1016/j.fsi.2019.04.018 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2019 Jun;89:378-383. doi: 10.1016/j.fsi.2019.04.018. Epub 
      2019 Apr 9.