PMID- 30978613
OWN - NLM
STAT- MEDLINE
DCOM- 20191120
LR  - 20191120
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 179
DP  - 2019 Jul 1
TI  - Organic-inorganic nanocomposites fabricated via functional ionic liquid as the 
      bridging agent for Laccase immobilization and its application in 
      2,4-dichlorophenol removal.
PG  - 260-269
LID - S0927-7765(19)30225-5 [pii]
LID - 10.1016/j.colsurfb.2019.04.002 [doi]
AB  - In this study, organic-inorganic nanocomposite including mesoporous silica SBA-15 
      and chitosan was combined using carboxyl-functionalized ionic liquid as the 
      bridging agent (SBA-CIL-CS), and which was characterized by scanning electron 
      microscope, Fourier transform infrared spectroscopy, thermogravimetric analysis, 
      and nitrogen adsorption-desorption isotherms. Thus prepared nanocomposite was 
      applied to laccase immobilization via physical adsorption (SBA-CIL-CS-Lac) and 
      utilized in 2,4-dichlorophenol (2,4-DCP) removal for the first time. 
      SBA-CIL-CS-Lac showed relatively high immobilization efficiency, activity 
      retention (75.3%), pH stability and storage stability. Kinetic experiment showed 
      SBA-CIL-CS-Lac had the eminent affinity to the substrate. Circular dichroism and 
      fluorescence analysis verified the active conformation of SBA-CIL-CS-Lac was 
      maintained well to keep its enzymatic activity. Compared with the previous 
      methods, SBA-CIL-CS-Lac exhibited prominent catalytic efficiency and reusability 
      as well as pH and temperature stability for 2,4-DCP removal. The removal rate of 
      SBA-CIL-CS-Lac for 2,4-DCP could be up to  90% after 35 h. Moreover, 58.8% 
      removal rate could be retained after five operation runs. These results indicated 
      that organic-inorganic nanocomposite SBA-CIL-CS was an ideal support for enzyme 
      immobilization.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Qiu, Xiang
AU  - Qiu X
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 210009, China.
FAU - Qin, Jing
AU  - Qin J
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 210009, China.
FAU - Xu, Min
AU  - Xu M
AD  - College of Pharmaceutical Science, Zhejiang University of Technology, Hangzhou, 
      310014, China.
FAU - Kang, Lifeng
AU  - Kang L
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 210009, China.
FAU - Hu, Yi
AU  - Hu Y
AD  - State Key Laboratory of Materials-Oriented Chemical Engineering, School of 
      Pharmaceutical Sciences, Nanjing Tech University, Nanjing, 210009, China; 
      Shandong Key Laboratory of Water Pollution Control and Resource Reuse, China. 
      Electronic address: huyi@njtech.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20190403
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Chlorophenols)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Inorganic Chemicals)
RN  - 0 (Ionic Liquids)
RN  - 0 (Organic Chemicals)
RN  - EC 1.10.3.2 (Laccase)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Biocatalysis
MH  - Chlorophenols/*isolation & purification
MH  - Enzyme Stability
MH  - Enzymes, Immobilized/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Inorganic Chemicals/*chemistry
MH  - Ionic Liquids/*chemistry
MH  - Kinetics
MH  - Laccase/chemistry/*metabolism
MH  - Nanocomposites/*chemistry/ultrastructure
MH  - Organic Chemicals/*chemistry
MH  - Protein Structure, Secondary
MH  - Spectrometry, Fluorescence
MH  - Temperature
OTO - NOTNLM
OT  - 2,4-dichlorophenol
OT  - Chitosan
OT  - Ionic liquid
OT  - Laccase immobilization
OT  - Mesoporous silica
EDAT- 2019/04/13 06:00
MHDA- 2019/11/21 06:00
CRDT- 2019/04/13 06:00
PHST- 2019/01/24 00:00 [received]
PHST- 2019/03/16 00:00 [revised]
PHST- 2019/04/02 00:00 [accepted]
PHST- 2019/04/13 06:00 [pubmed]
PHST- 2019/11/21 06:00 [medline]
PHST- 2019/04/13 06:00 [entrez]
AID - S0927-7765(19)30225-5 [pii]
AID - 10.1016/j.colsurfb.2019.04.002 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2019 Jul 1;179:260-269. doi: 
      10.1016/j.colsurfb.2019.04.002. Epub 2019 Apr 3.