PMID- 30983000
OWN - NLM
STAT- MEDLINE
DCOM- 20200812
LR  - 20200812
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 8
DP  - 2019 Aug
TI  - A new subset of small stem cells in bovine bone marrow stromal cell populations.
PG  - 13881-13892
LID - 10.1002/jcb.28661 [doi]
AB  - Bone marrow stromal cells (BMSCs) are a unique population of multipotent cells 
      that exhibit pluripotent properties to a certain extent and are significantly 
      heterogeneous in terms of the cell population. We isolate a small cell 
      subpopulation from bovine BMSCs, bovine small stem cells (bSSCs), and herein 
      characterize their properties. The bSSCs are smaller in size and express nuclear 
      Oct-4 and other pluripotency markers. In addition, when cultured in suspension 
      conditions, bSSCs form three-dimensional spheres and display a strong capability 
      for self-renewal and differentiation into cells from three germ layers. Notably, 
      bSSCs display neural features with Sox1 and Pax6 expression. Using bSSCs as donor 
      nuclear cells for somatic cell nuclear transfer, we further demonstrate that the 
      developmental potential of cloned embryos in vitro is significantly increased. 
      Our study identifies a new bovine bone marrow stromal cell-derived stem cell 
      subtype that could have broad importance for developmental biology as well as 
      great potential for regenerative medicine.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Pan, Shaohui
AU  - Pan S
AUID- ORCID: 0000-0002-3615-2917
AD  - Laboratory for Stem Cell and Retinal Regeneration, Institute of Stem Cell 
      Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical 
      University, Wenzhou, China.
AD  - State Key Laboratory of Ophthalmology, Optometry and Visual Sciences, Wenzhou, 
      China.
FAU - Chen, Yu-Chen
AU  - Chen YC
AD  - Laboratory for Stem Cell and Retinal Regeneration, Institute of Stem Cell 
      Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical 
      University, Wenzhou, China.
AD  - State Key Laboratory of Ophthalmology, Optometry and Visual Sciences, Wenzhou, 
      China.
FAU - Zhao, Ning
AU  - Zhao N
AD  - Laboratory for Stem Cell and Retinal Regeneration, Institute of Stem Cell 
      Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical 
      University, Wenzhou, China.
AD  - State Key Laboratory of Ophthalmology, Optometry and Visual Sciences, Wenzhou, 
      China.
FAU - Feng, Xiang
AU  - Feng X
AD  - Laboratory for Stem Cell and Retinal Regeneration, Institute of Stem Cell 
      Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical 
      University, Wenzhou, China.
AD  - State Key Laboratory of Ophthalmology, Optometry and Visual Sciences, Wenzhou, 
      China.
FAU - Yang, Dan-Dan
AU  - Yang DD
AD  - Laboratory for Stem Cell and Retinal Regeneration, Institute of Stem Cell 
      Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical 
      University, Wenzhou, China.
AD  - State Key Laboratory of Ophthalmology, Optometry and Visual Sciences, Wenzhou, 
      China.
FAU - Wang, Yongshen
AU  - Wang Y
AD  - Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of 
      Veterinary Medicine, Northwest A&F University, Yangling, China.
FAU - Jin, Zi-Bing
AU  - Jin ZB
AD  - Laboratory for Stem Cell and Retinal Regeneration, Institute of Stem Cell 
      Research, Division of Ophthalmic Genetics, The Eye Hospital, Wenzhou Medical 
      University, Wenzhou, China.
AD  - State Key Laboratory of Ophthalmology, Optometry and Visual Sciences, Wenzhou, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190415
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Biomarkers)
RN  - 0 (Octamer Transcription Factor-3)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers/metabolism
MH  - Blastocyst/cytology
MH  - Cattle
MH  - Cell Count
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cell Self Renewal
MH  - Cell Separation
MH  - *Cell Size
MH  - Cloning, Organism
MH  - Embryo, Mammalian/cytology
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Neural Plate/cytology
MH  - Octamer Transcription Factor-3/metabolism
OTO - NOTNLM
OT  - bovine
OT  - differentiation potential
OT  - mesenchymal stromal cell
OT  - multipotency
OT  - small stem cells
EDAT- 2019/04/16 06:00
MHDA- 2020/08/13 06:00
CRDT- 2019/04/16 06:00
PHST- 2018/08/06 00:00 [received]
PHST- 2018/12/21 00:00 [revised]
PHST- 2019/01/07 00:00 [accepted]
PHST- 2019/04/16 06:00 [pubmed]
PHST- 2020/08/13 06:00 [medline]
PHST- 2019/04/16 06:00 [entrez]
AID - 10.1002/jcb.28661 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Aug;120(8):13881-13892. doi: 10.1002/jcb.28661. Epub 2019 
      Apr 15.