PMID- 30984042
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220410
IS  - 1664-0640 (Print)
IS  - 1664-0640 (Electronic)
IS  - 1664-0640 (Linking)
VI  - 10
DP  - 2019
TI  - SiNiSan Ameliorates the Depression-Like Behavior of Rats That Experienced 
      Maternal Separation Through 5-HT1A Receptor/CREB/BDNF Pathway.
PG  - 160
LID - 10.3389/fpsyt.2019.00160 [doi]
LID - 160
AB  - Background: Early adverse life stress is an important dangerous factor in the 
      development of psychiatric disorders, particularly depression. Available clinical 
      antidepressant agents, such as fluoxetine, [a selective serotonin reuptake 
      inhibitor (SSRI)], are unsatisfactory because of their side effects. SiNiSan 
      (SNS) is a classic Chinese medicine prescription regarded to disperse stagnated 
      liver qi to relieve qi stagnation. Therefore, this study was designed to detect 
      the effects and molecular mechanism of SNS treatment in rats subjected to 
      maternal separation (MS). Method: Male neonatal Wistar rats were divided into six 
      groups including control + ddH(2)O, MS + ddH(2)O, MS + fluoxetine (5 g/kg), MS + 
      SNS -low dose (2.5 g/kg), MS + SNS -medium dose (5 g/kg), MS + SNS -high dose (10 
      g/kg). The volume of drugs and ddH2O in each group are according to the weight of 
      rats every day (10 mL/kg). Each group comprised 16 pups with 8 young and 8 adult 
      pups. Except for the control group, all MS groups were separated from their 
      mothers for 4 h/day from 9:00 to 13:00 during postnatal days (PNDs) 1 to 21. 
      After MS, the six groups were intragastrically administered with ddH2O, 
      fluoxetine, and different doses of SNS until PND 28 (for young pups) and PND 56 
      (for adult pups). The pups were weighed every day, and depression-like behavior 
      was assessed by sucrose preference test, open field test, and forced swimming 
      test. Serotonin 1A (5-HT1A) receptor, phosphorylated protein kinase A (p-PKA) 
      substrate, cAMP response element-binding protein (CREB), p-CREB and brain-derived 
      neurotrophic factor (BDNF) in the hippocampus were examined by Western blot, and 
      in situ 5-HT1A receptor expression was measured by IHC. Results: Young and adult 
      MS rats exhibited depression-like behavior. However, the depression-like behavior 
      was ameliorated by SNS in both age groups. The levels of 5-HT1A receptor, p-CREB, 
      and BDNF in the hippocampus were reduced in young and adult MS rats. SNS 
      treatment significantly up-regulated the expression of 5-HT1A receptor, p-CREB, 
      and BDNF in the hippocampus of adult MS rats. However, few significant effects on 
      the protein expression were observed in the young MS rats. Conclusion: MS in 
      infancy could develop depression-like behavior in young and adult. SNS treatment 
      may perform antidepressant effects on young and adult MS rats through the 
      BDNF/PKA/CREB pathway.
FAU - Cao, Kerun
AU  - Cao K
AD  - School of Fundamental Medical Science, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Shen, Chongkun
AU  - Shen C
AD  - School of Fundamental Medical Science, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Yuan, Yumei
AU  - Yuan Y
AD  - Shenzhen Baoan Hospital of Chinese Medicine, Shenzhen, China.
FAU - Bai, Shasha
AU  - Bai S
AD  - Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Yang, Lei
AU  - Yang L
AD  - Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Guo, Lili
AU  - Guo L
AD  - Third Affiliated Hospital of Henan University of Chinese Medicine, Henan 
      University of Chinese Medicine, Zhengzhou, China.
FAU - Zhang, Rong
AU  - Zhang R
AD  - Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
FAU - Shi, Yafei
AU  - Shi Y
AD  - School of Fundamental Medical Science, Guangzhou University of Chinese Medicine, 
      Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20190328
PL  - Switzerland
TA  - Front Psychiatry
JT  - Frontiers in psychiatry
JID - 101545006
PMC - PMC6447714
OTO - NOTNLM
OT  - SiNiSan
OT  - brain-derived neurotrophic factor
OT  - depression
OT  - early life stress
OT  - serotonin 1A receptor
EDAT- 2019/04/16 06:00
MHDA- 2019/04/16 06:01
PMCR- 2019/03/28
CRDT- 2019/04/16 06:00
PHST- 2018/09/14 00:00 [received]
PHST- 2019/03/04 00:00 [accepted]
PHST- 2019/04/16 06:00 [entrez]
PHST- 2019/04/16 06:00 [pubmed]
PHST- 2019/04/16 06:01 [medline]
PHST- 2019/03/28 00:00 [pmc-release]
AID - 10.3389/fpsyt.2019.00160 [doi]
PST - epublish
SO  - Front Psychiatry. 2019 Mar 28;10:160. doi: 10.3389/fpsyt.2019.00160. eCollection 
      2019.