PMID- 30984289
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231006
IS  - 1755-8166 (Print)
IS  - 1755-8166 (Electronic)
IS  - 1755-8166 (Linking)
VI  - 12
DP  - 2019
TI  - The karyotype of Aegilops geniculata and its use to identify both addition and 
      substitution lines of wheat.
PG  - 15
LID - 10.1186/s13039-019-0428-2 [doi]
LID - 15
AB  - BACKGROUND: The annual allotetraploid species Aegilops geniculata harbors a 
      number of traits relevant for wheat improvement. An effective cytogenetic method 
      has yet to be developed to distinguish between each of its 14 chromosomes. 
      RESULTS: A fluorescence in situ hybridization (FISH) approach was adopted to 
      describe the karyotype of Ae. geniculata. Each of its 14 chromosomes was 
      unequivocally recognized using a cocktail of three probes, namely pTa-713, 
      (AAC)(5) and pTa71. FISH karyotyping was then used to detect and characterize 
      selections from an Ae. geniculata x bread wheat wide cross of a chromosome 1M(g) 
      disomic addition line and three 4M(g)(4B) substitution lines. The identity of the 
      addition line was confirmed by the presence of Glu-M1, detected both using an 
      SDS-PAGE separation of endosperm proteins and by applying a PCR assay directed at 
      the Glu-M1 locus. The status of the substitution lines was validated by 
      genotyping using a wheat single nucleotide polymorphism chip. CONCLUSION: FISH 
      karyotyping based on pTa-713, (AAC)(5) and pTa71 will be useful for determining 
      the contribution of Ae. geniculata to derivatives of an Ae. geniculata x wheat 
      wide cross. SNP chip-based genotyping is effective for confirming the status of 
      whole chromosome wheat/alien substitution lines.
FAU - Yi, Yingjin
AU  - Yi Y
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Zheng, Ke
AU  - Zheng K
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Ning, Shunzong
AU  - Ning S
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Zhao, Laibin
AU  - Zhao L
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Xu, Kai
AU  - Xu K
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Hao, Ming
AU  - Hao M
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Zhang, Lianquan
AU  - Zhang L
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Yuan, Zhongwei
AU  - Yuan Z
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
FAU - Liu, Dengcai
AU  - Liu D
AUID- ORCID: 0000-0001-5508-1125
AD  - Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130 
      Sichuan China. ISNI: 0000 0001 0185 3134. GRID: grid.80510.3c
LA  - eng
PT  - Journal Article
DEP - 20190402
PL  - England
TA  - Mol Cytogenet
JT  - Molecular cytogenetics
JID - 101317942
PMC - PMC6446333
OTO - NOTNLM
OT  - Addition line
OT  - Fluorescence in situ hybridization
OT  - Single nucleotide polymorphism
OT  - Substitution line
COIS- Not applicable.Not applicable.The authors declare that they have no competing 
      interests.Springer Nature remains neutral with regard to jurisdictional claims in 
      published maps and institutional affiliations.
EDAT- 2019/04/16 06:00
MHDA- 2019/04/16 06:01
PMCR- 2019/04/02
CRDT- 2019/04/16 06:00
PHST- 2018/12/24 00:00 [received]
PHST- 2019/03/26 00:00 [accepted]
PHST- 2019/04/16 06:00 [entrez]
PHST- 2019/04/16 06:00 [pubmed]
PHST- 2019/04/16 06:01 [medline]
PHST- 2019/04/02 00:00 [pmc-release]
AID - 428 [pii]
AID - 10.1186/s13039-019-0428-2 [doi]
PST - epublish
SO  - Mol Cytogenet. 2019 Apr 2;12:15. doi: 10.1186/s13039-019-0428-2. eCollection 
      2019.