PMID- 30986097
OWN - NLM
STAT- MEDLINE
DCOM- 20190715
LR  - 20190715
IS  - 1945-0257 (Electronic)
IS  - 1945-0257 (Linking)
VI  - 23
IP  - 4
DP  - 2019 Apr
TI  - Identification of an Exosomal Long Noncoding RNA SOX2-OT in Plasma as a Promising 
      Biomarker for Lung Squamous Cell Carcinoma.
PG  - 235-240
LID - 10.1089/gtmb.2018.0103 [doi]
AB  - AIMS: Evaluation of nucleic acids in plasma exosomes is a noninvasive method that 
      can be used to detect different types of cancer. The aim of this study was to 
      determine the value of exosomal long noncoding RNAs (lncRNAs) in detecting lung 
      squamous cell carcinoma (LSCC). MATERIALS AND METHODS: A total of 75 LSCC 
      patients and 79 negative control subjects were enrolled in the study. Twenty 
      differentially expressed lncRNAs were evaluated as potential candidates. Exosomes 
      were isolated by ultracentrifugation, and lncRNA levels in exosomes were 
      determined using real-time polymerase chain reaction. Receiver Operating 
      Characteristic (ROC) curves were used to determine specificity and sensitivity. 
      RESULTS: Exosomal SOX2-OT was significantly upregulated in LSCC patients and 
      showed the strongest power in detecting LSCC. The area under the ROC curve was 
      0.815, and the sensitivity and specificity were 76% and 73.17%, respectively. 
      Moreover, exosomal SOX2-OT levels were significantly correlated with tumor size, 
      TNM stage, and lymph node metastasis. Exosomal SOX2-OT levels were significantly 
      decreased in the postoperative plasma of LSCC patients. CONCLUSION: SOX2-OT may 
      serve as a promising noninvasive plasma-based tumor biomarker for LSCC.
FAU - Teng, Yun
AU  - Teng Y
AD  - Department of Laboratory Medicine, The First Affiliated Hospital of China Medical 
      University, Shenyang, P.R. China.
FAU - Kang, Hui
AU  - Kang H
AD  - Department of Laboratory Medicine, The First Affiliated Hospital of China Medical 
      University, Shenyang, P.R. China.
FAU - Chu, Yang
AU  - Chu Y
AD  - Department of Laboratory Medicine, The First Affiliated Hospital of China Medical 
      University, Shenyang, P.R. China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genet Test Mol Biomarkers
JT  - Genetic testing and molecular biomarkers
JID - 101494210
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SOX2 protein, human)
RN  - 0 (SOXB1 Transcription Factors)
RN  - EC 3.1.- (Exosome Multienzyme Ribonuclease Complex)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics
MH  - Carcinoma, Non-Small-Cell Lung/genetics
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Exosome Multienzyme Ribonuclease Complex/blood/*genetics
MH  - Exosomes/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Genes, Overlapping/genetics
MH  - Humans
MH  - Lung Neoplasms/diagnosis/genetics
MH  - Lymphatic Metastasis
MH  - Male
MH  - Middle Aged
MH  - RNA, Long Noncoding/analysis/genetics
MH  - ROC Curve
MH  - SOXB1 Transcription Factors/*genetics
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - SOX2-OT
OT  - biomarker
OT  - exosome
OT  - long noncoding RNA
OT  - lung squamous cell carcinoma
EDAT- 2019/04/16 06:00
MHDA- 2019/07/16 06:00
CRDT- 2019/04/16 06:00
PHST- 2019/04/16 06:00 [entrez]
PHST- 2019/04/16 06:00 [pubmed]
PHST- 2019/07/16 06:00 [medline]
AID - 10.1089/gtmb.2018.0103 [doi]
PST - ppublish
SO  - Genet Test Mol Biomarkers. 2019 Apr;23(4):235-240. doi: 10.1089/gtmb.2018.0103.