PMID- 30986273
OWN - NLM
STAT- MEDLINE
DCOM- 20200107
LR  - 20200309
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 14
IP  - 4
DP  - 2019
TI  - Prevalence of kdr mutations and insecticide susceptibility among natural 
      population of Aedes aegypti in West Bengal.
PG  - e0215541
LID - 10.1371/journal.pone.0215541 [doi]
LID - e0215541
AB  - BACKGROUND: Aedes albopictus and Aedes aegypti are the major vectors of arboviral 
      diseases. As effective vaccines are not available for most of the arboviral 
      diseases, vector control by using insecticides play the key role to reduce the 
      disease transmission. The emergence and spread of resistance to different classes 
      of insecticides by the vectors is a major obstacle to control the disease 
      transmission. Information about vector susceptibility to different insecticides 
      and their mechanisms are very important for formulating proper vector control 
      measures. The present study was designed to assess the susceptibility of Ae. 
      aegypti against three different classes of adulticides, one larvicidal agent 
      available and polymorphisms in the voltage-gated sodium channel (VGSC) gene 
      related to insecticide resistance. METHODS: Immature stages of Ae. aegypti were 
      collected from three dengue endemic municipal areas of West Bengal and reared in 
      the laboratory. Larvae and adults (F1 progeny) were used for insecticide bioassay 
      as per WHO protocols. Knock down resistance gene (kdr) mutations were assessed by 
      direct sequencing of PCR products. RESULTS: The Ae. aegypti population was found 
      to be susceptible to type II pyrethroids and malathion but highly resistant to 
      DDT. A high rate of polymorphisms in the VGSC gene was observed among the 
      collected mosquitoes. A double mutant V1016G + F1534C was found to be associated 
      with DDT resistance but neither V1016G nor F1534C alone showed the same 
      association. Association between the kdr mutations and the susceptibility status 
      of pyrethroids could not be established due to very small sample size. A low to 
      moderate level of resistance was noticed against temephos among the larval 
      population based on WHO criteria. CONCLUSION: The replacement of DDT by type II 
      pyrethroids for the management of dengue vectors is an appropriate decision taken 
      by the national program which is supported by the findings of a higher level of 
      resistance to DDT. Persistence of polymorphisms in the VGSC gene might be an 
      indication of emergence of resistance against pyrethroid insecticides that should 
      be monitored at a regular interval. Attempts should be made to determine the 
      effectiveness of other larvicides for replacement of temephos if needed in 
      future. Along with the chemical insecticides different biological vector control 
      methods as well as biopesticides should also be used in vector control 
      programmes.
FAU - Saha, Pabitra
AU  - Saha P
AD  - Department of Microbiology, Calcutta School of Tropical Medicine, Kolkata, West 
      Bengal, India.
AD  - Department of Zoology, A. P. C. Roy Govt. College, Himachal Bihar, Matigara, 
      Siliguri, West Bengal, India.
FAU - Chatterjee, Moytrey
AU  - Chatterjee M
AD  - Department of Microbiology, Calcutta School of Tropical Medicine, Kolkata, West 
      Bengal, India.
FAU - Ballav, Sudeep
AU  - Ballav S
AD  - Department of Microbiology, Calcutta School of Tropical Medicine, Kolkata, West 
      Bengal, India.
FAU - Chowdhury, Akash
AU  - Chowdhury A
AD  - Department of Zoology, A. P. C. Roy Govt. College, Himachal Bihar, Matigara, 
      Siliguri, West Bengal, India.
FAU - Basu, Nandita
AU  - Basu N
AD  - Department of Pathology, Jagannath Gupta Institute of Medical Sciences and 
      Hospital, Budge Budge, Kolkata, West Bengal, India.
FAU - Maji, Ardhendu Kumar
AU  - Maji AK
AD  - Department of Microbiology, Calcutta School of Tropical Medicine, Kolkata, West 
      Bengal, India.
LA  - eng
PT  - Journal Article
DEP - 20190415
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Insect Proteins)
RN  - 0 (Insecticides)
RN  - 0 (Pyrethrins)
RN  - 0 (Voltage-Gated Sodium Channels)
SB  - IM
MH  - *Aedes/enzymology/genetics
MH  - Animals
MH  - Drug Resistance/drug effects/*genetics
MH  - India
MH  - *Insect Proteins/genetics/metabolism
MH  - Insecticides/*pharmacology
MH  - *Mutation
MH  - Prevalence
MH  - Pyrethrins/*pharmacology
MH  - *Voltage-Gated Sodium Channels/genetics/metabolism
PMC - PMC6464230
COIS- The authors have declared that no competing interests exist.
EDAT- 2019/04/16 06:00
MHDA- 2020/01/08 06:00
PMCR- 2019/04/15
CRDT- 2019/04/16 06:00
PHST- 2018/11/25 00:00 [received]
PHST- 2019/04/03 00:00 [accepted]
PHST- 2019/04/16 06:00 [entrez]
PHST- 2019/04/16 06:00 [pubmed]
PHST- 2020/01/08 06:00 [medline]
PHST- 2019/04/15 00:00 [pmc-release]
AID - PONE-D-18-33652 [pii]
AID - 10.1371/journal.pone.0215541 [doi]
PST - epublish
SO  - PLoS One. 2019 Apr 15;14(4):e0215541. doi: 10.1371/journal.pone.0215541. 
      eCollection 2019.