PMID- 30987723
OWN - NLM
STAT- MEDLINE
DCOM- 20200529
LR  - 20220809
IS  - 2224-6614 (Electronic)
IS  - 1021-9498 (Print)
VI  - 27
IP  - 2
DP  - 2019 Apr
TI  - Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis.
PG  - 518-525
LID - 10.1016/j.jfda.2019.03.001 [doi]
AB  - Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic 
      hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the 
      ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts 
      with cellular DNA to form BPDE-dG adducts responsible for BaP-induced 
      mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our 
      developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 
      normal human umbilical cord blood samples and 42 birth defect cases. We 
      determined that there is no significant difference in the level of BPDE-dG 
      formation between the normal and birth defect groups. This represents the first 
      time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood 
      samples. The results indicated that under experimental conditions, BPDE-dG 
      adducts were detected in all the human umbilical cord blood samples from the 
      normal and birth defect groups.
CI  - Copyright (c) 2019. Published by Elsevier Taiwan LLC.
FAU - Guo, Ling
AU  - Guo L
AD  - Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control 
      and Prevention, Chinese Center for Disease Control and Prevention, 100050 
      Beijing, China.
AD  - National Institute of Occupational Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, 100050 Beijing, China.
AD  - Department of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
FAU - Jiang, Xiao
AU  - Jiang X
AD  - Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control 
      and Prevention, Chinese Center for Disease Control and Prevention, 100050 
      Beijing, China.
AD  - National Institute of Occupational Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, 100050 Beijing, China.
FAU - Tian, Hao-Yuan
AU  - Tian HY
AD  - Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control 
      and Prevention, Chinese Center for Disease Control and Prevention, 100050 
      Beijing, China.
AD  - National Institute of Occupational Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, 100050 Beijing, China.
FAU - Yao, Shang-Jin
AU  - Yao SJ
AD  - Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control 
      and Prevention, Chinese Center for Disease Control and Prevention, 100050 
      Beijing, China.
AD  - National Institute of Occupational Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, 100050 Beijing, China.
AD  - Department of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
FAU - Li, Bo-Ya
AU  - Li BY
AD  - Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control 
      and Prevention, Chinese Center for Disease Control and Prevention, 100050 
      Beijing, China.
AD  - National Institute of Occupational Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, 100050 Beijing, China.
FAU - Zhang, Rong-Jie
AU  - Zhang RJ
AD  - Henan Center for Disease Control and Prevention, Zhengzhou 450016, China.
FAU - Zhang, Shu-Sheng
AU  - Zhang SS
AD  - Department of Chemistry, Zhengzhou University, Zhengzhou 450001, China.
FAU - Sun, Xin
AU  - Sun X
AD  - Key Laboratory of Chemical Safety and Health, Chinese Center for Disease Control 
      and Prevention, Chinese Center for Disease Control and Prevention, 100050 
      Beijing, China.
AD  - National Institute of Occupational Health and Poison Control, Chinese Center for 
      Disease Control and Prevention, 100050 Beijing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190323
PL  - China (Republic : 1949- )
TA  - J Food Drug Anal
JT  - Journal of food and drug analysis
JID - 101630927
RN  - 0 (DNA Adducts)
RN  - 0 (benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA)
RN  - 55097-80-8 (7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide)
SB  - IM
MH  - 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide/chemistry
MH  - Chromatography, Liquid
MH  - DNA Adducts/*blood/chemistry
MH  - Female
MH  - Fetal Blood/*chemistry
MH  - Humans
MH  - Molecular Conformation
MH  - Pregnancy
MH  - Tandem Mass Spectrometry
PMC - PMC9296209
OTO - NOTNLM
OT  - BPDE-dG
OT  - Human biomonitoring
OT  - LC-MS/MS
OT  - Molecular epidemiology
COIS- Conflicts of interest The authors declare no conflict of interest.
EDAT- 2019/04/17 06:00
MHDA- 2020/05/30 06:00
PMCR- 2019/03/23
CRDT- 2019/04/17 06:00
PHST- 2019/02/27 00:00 [received]
PHST- 2019/02/27 00:00 [accepted]
PHST- 2019/04/17 06:00 [entrez]
PHST- 2019/04/17 06:00 [pubmed]
PHST- 2020/05/30 06:00 [medline]
PHST- 2019/03/23 00:00 [pmc-release]
AID - j.jfda.2019.03.001 [pii]
AID - jfda-27-02-518 [pii]
AID - 10.1016/j.jfda.2019.03.001 [doi]
PST - ppublish
SO  - J Food Drug Anal. 2019 Apr;27(2):518-525. doi: 10.1016/j.jfda.2019.03.001. Epub 
      2019 Mar 23.