PMID- 30988364
OWN - NLM
STAT- MEDLINE
DCOM- 20201015
LR  - 20231011
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 Apr 15
TI  - Different effects of prenatal MAM vs. perinatal THC exposure on regional cerebral 
      blood perfusion detected by Arterial Spin Labelling MRI in rats.
PG  - 6062
LID - 10.1038/s41598-019-42532-z [doi]
LID - 6062
AB  - Clinical studies consistently report structural impairments (i.e.: ventricular 
      enlargement, decreased volume of anterior cingulate cortex or hippocampus) and 
      functional abnormalities including changes in regional cerebral blood flow in 
      individuals suffering from schizophrenia, which can be evaluated by magnetic 
      resonance imaging (MRI) techniques. The aim of this study was to assess cerebral 
      blood perfusion in several schizophrenia-related brain regions using Arterial 
      Spin Labelling MRI (ASL MRI, 9.4 T Bruker BioSpec 94/30USR scanner) in rats. In 
      this study, prenatal exposure to methylazoxymethanol acetate (MAM, 22 mg/kg) at 
      gestational day (GD) 17 and the perinatal treatment with Delta-9-tetrahydrocannabinol 
      (THC, 5 mg/kg) from GD15 to postnatal day 9 elicited behavioral deficits 
      consistent with schizophrenia-like phenotype, which is in agreement with the 
      neurodevelopmental hypothesis of schizophrenia. In MAM exposed rats a significant 
      enlargement of lateral ventricles and perfusion changes (i.e.: increased blood 
      perfusion in the circle of Willis and sensorimotor cortex and decreased perfusion 
      in hippocampus) were detected. On the other hand, the THC perinatally exposed 
      rats did not show differences in the cerebral blood perfusion in any region of 
      interest. These results suggest that although both pre/perinatal insults showed 
      some of the schizophrenia-like deficits, these are not strictly related to 
      distinct hemodynamic features.
FAU - Drazanova, Eva
AU  - Drazanova E
AD  - Department of Pharmacology, Faculty of Medicine, Masaryk University, Brno, Czech 
      Republic. edrazan@isibrno.cz.
AD  - Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech 
      Republic. edrazan@isibrno.cz.
FAU - Ruda-Kucerova, Jana
AU  - Ruda-Kucerova J
AUID- ORCID: 0000-0002-1846-0799
AD  - Department of Pharmacology, Faculty of Medicine, Masaryk University, Brno, Czech 
      Republic.
FAU - Kratka, Lucie
AU  - Kratka L
AD  - Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech 
      Republic.
AD  - Department of Biomedical Engineering, Faculty of Electrical Engineering and 
      Communication, University of Technology, Brno, Czech Republic.
FAU - Stark, Tibor
AU  - Stark T
AUID- ORCID: 0000-0001-8118-9914
AD  - Department of Pharmacology, Faculty of Medicine, Masaryk University, Brno, Czech 
      Republic.
FAU - Kuchar, Martin
AU  - Kuchar M
AD  - Forensic Laboratory of Biologically Active Substances, Department of Chemistry of 
      Natural Compounds, University of Chemistry and Technology Prague, Prague, Czech 
      Republic.
FAU - Maryska, Michal
AU  - Maryska M
AUID- ORCID: 0000-0001-9593-9472
AD  - Forensic Laboratory of Biologically Active Substances, Department of Chemistry of 
      Natural Compounds, University of Chemistry and Technology Prague, Prague, Czech 
      Republic.
FAU - Drago, Filippo
AU  - Drago F
AD  - Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, 
      School of Medicine, University of Catania, Catania, Italy.
FAU - Starcuk, Zenon Jr
AU  - Starcuk Z Jr
AD  - Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech 
      Republic.
FAU - Micale, Vincenzo
AU  - Micale V
AD  - Department of Biomedical and Biotechnological Sciences, Section of Pharmacology, 
      School of Medicine, University of Catania, Catania, Italy.
AD  - National Institute of Mental Health, Klecany, Czech Republic.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190415
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 592-62-1 (Methylazoxymethanol Acetate)
RN  - 7J8897W37S (Dronabinol)
SB  - IM
MH  - Animals
MH  - Behavior Observation Techniques
MH  - Cerebrovascular Circulation/drug effects
MH  - Circle of Willis/diagnostic imaging/drug effects/embryology
MH  - Disease Models, Animal
MH  - Dronabinol/*toxicity
MH  - Female
MH  - Hippocampus/blood supply/diagnostic imaging/drug effects/embryology
MH  - Humans
MH  - Magnetic Resonance Angiography/methods
MH  - Male
MH  - Methylazoxymethanol Acetate/*toxicity
MH  - Neurogenesis/*drug effects
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects/chemically induced/*diagnostic imaging
MH  - Rats
MH  - Schizophrenia/*chemically induced/diagnosis
MH  - Sensorimotor Cortex/blood supply/diagnostic imaging/drug effects/embryology
PMC - PMC6465353
COIS- The authors declare no competing interests.
EDAT- 2019/04/17 06:00
MHDA- 2020/10/21 06:00
PMCR- 2019/04/15
CRDT- 2019/04/17 06:00
PHST- 2018/08/22 00:00 [received]
PHST- 2019/04/02 00:00 [accepted]
PHST- 2019/04/17 06:00 [entrez]
PHST- 2019/04/17 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2019/04/15 00:00 [pmc-release]
AID - 10.1038/s41598-019-42532-z [pii]
AID - 42532 [pii]
AID - 10.1038/s41598-019-42532-z [doi]
PST - epublish
SO  - Sci Rep. 2019 Apr 15;9(1):6062. doi: 10.1038/s41598-019-42532-z.