PMID- 30988743
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 17
IP  - 5
DP  - 2019 May
TI  - Effect of T-cadherin on the AKT/mTOR signaling pathway, gastric cancer cell 
      cycle, migration and invasion, and its association with patient survival rate.
PG  - 3607-3613
LID - 10.3892/etm.2019.7350 [doi]
AB  - Gastric cancer (GC) is among the most common types of human cancer and is 
      associated with recurrence and metastasis, despite comprehensive surgical and 
      medical treatment. Previous studies observed downregulation of T-cadherin 
      expression in GC tissues, suggesting that this protein may act as an 
      oncosuppressor. The current study investigated the activity of T-cadherin in GC 
      tissues. In a follow-up study of 81 patients with GC, a Kaplan-Meier analysis of 
      overall survival revealed a strong association of T-cadherin overexpression with 
      increased overall survival (P<0.01). Furthermore, stable 
      T-cadherin-overexpressing cell lines were established from HGC-27 cells via 
      transfection of a pcDNA3.1-T-cadherin plasmid and in vitro growth and cell cycle 
      of these cells were measured using MTT and flow cytometry assays, respectively. 
      MTT assays revealed that proliferation of engineered T-cadherin-overexpressing 
      cells was significantly inhibited and flow cytometry demonstrated that T-cadherin 
      overexpression in HGC-27 cells induced cell cycle arrest in the G(0)/G(1) phase. 
      Transwell assays demonstrated that T-cadherin-overexpressing HGC-27 cells 
      exhibited reduced invasiveness and metastatic potential. Phosphorylated 
      (p)-protein kinase B (AKT) and p-mammalian target of rapamycin (mTOR) protein 
      levels were reduced in T-cadherin overexpressing HGC-27 cells, suggesting that 
      the AKT/mTOR signaling pathway was involved in the gastric tumor inhibitory 
      effect of T-cadherin. Administration of AKT-activator, insulin-like growth 
      factor-1, to T-cadherin-overexpressing HGC-27 cells significantly affected the 
      proliferation phenotype. In conclusion, the current study provided clinical 
      evidence and revealed a potential mechanism supporting that T-cadherin inhibits 
      gastric tumorigenesis through inhibition of the AKT/mTOR signaling pathway.
FAU - Lin, Jianqing
AU  - Lin J
AD  - Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Chen, Zhiyao
AU  - Chen Z
AD  - Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Huang, Zhijun
AU  - Huang Z
AD  - Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Chen, Feng
AU  - Chen F
AD  - Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Ye, Zeyi
AU  - Ye Z
AD  - Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Lin, Shaoze
AU  - Lin S
AD  - Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
FAU - Wang, Weidong
AU  - Wang W
AD  - Department of Surgical Oncology, Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, Fujian 362000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190306
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6447793
OTO - NOTNLM
OT  - T-cadherin
OT  - gastric cancer
OT  - invasion
OT  - migration
OT  - overexpression
EDAT- 2019/04/17 06:00
MHDA- 2019/04/17 06:01
PMCR- 2019/03/06
CRDT- 2019/04/17 06:00
PHST- 2017/11/04 00:00 [received]
PHST- 2019/01/03 00:00 [accepted]
PHST- 2019/04/17 06:00 [entrez]
PHST- 2019/04/17 06:00 [pubmed]
PHST- 2019/04/17 06:01 [medline]
PHST- 2019/03/06 00:00 [pmc-release]
AID - ETM-0-0-7350 [pii]
AID - 10.3892/etm.2019.7350 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 May;17(5):3607-3613. doi: 10.3892/etm.2019.7350. Epub 2019 Mar 
      6.