PMID- 30989865
OWN - NLM
STAT- MEDLINE
DCOM- 20190814
LR  - 20190814
IS  - 1001-5302 (Print)
IS  - 1001-5302 (Linking)
VI  - 44
IP  - 5
DP  - 2019 Mar
TI  - [Effects of Jingfang n-butanol extraction isolated fraction A on LPS-induced 
      inflammation in RAW264.7 cells].
PG  - 1026-1033
LID - 10.19540/j.cnki.cjcmm.20181214.004 [doi]
AB  - The LPS-induced RAW264. 7 cells inflammation model was used as a carrier to 
      investigate the in vitro anti-inflammation effects of Jingfang n-butanol 
      extraction(JFNE) isolated fraction A and explore its preliminary 
      anti-inflammation mechanism by observing the regulatory effect on PI3 K/AKT 
      signaling pathway and NF-kappaB pathway. The RAW264. 7 cells inflammation model was 
      established by stimulating with LPS for 12 h. After 3 h pre-treatment with 
      fraction A,the contents of interleukin-6(IL-6),interleukin-1beta(IL-1beta) and tumor 
      necrosis factor(TNF-alpha) in the supernatant of RAW264. 7 cells inflammation model 
      were determined by ELISA and the contents of NO in supernatant were assayed by 
      Griess. Reverse transcription-polymerase chain reaction(RT-PCR) method was used 
      to determine the expression of IL-6,IL-1beta,TNF-alpha,IFN-gamma,i NOS,PI3 K,AKT,CHUK,NF-kappaB1 
      and Rela mRNA in RAW264. 7 inflammatory cells,and the expression levels of 
      phosphorylated and total PI3 K/AKT protein,NF-kappaB p50,p65,p-p65,p105 protein in 
      cells were determined via Western blot. In addition,LC-MS and database were used 
      to identify the possible chemical constituents in fraction A. The results showed 
      that fraction A could significantly reduce the release levels of NO,IL-6,IL-1beta 
      and TNF-alpha in the supernatant and the expression of IL-6,IL-1beta,TNF-alpha,IFN-gamma,i 
      NOS,PI3 K,AKT,CHUK,NF-kappaB1 and Rela mRNA in RAW264. 7 inflammation model 
      cells(P<0. 05 or P<0. 01) and significantly inhibit the phosphorylation 
      expression levels of PI3 K and AKT protein and mRNA expressions(P<0. 05 or P<0. 
      01). Moreover,fraction A could significantly reduce the levels of NF-kappaB p50,p-p65 
      and i NOS protein,as well as NF-kappaB1,Rela mRNA expressions in RAW264. 7 cells,and 
      increase the expression of CHUK gene.A total of 196 compounds were identified 
      from fraction A in the composition analysis,and 
      isoobtusilactone,5-O-methyl-vismitol,emebel(embelin) and prim-O-glucosylcimifugin 
      showed high contents. The results all above showed that fraction A had a certain 
      antiinflammatory effect in LPS-induced RAW264. 7 inflammation model cells,and its 
      anti-inflammatory effects may be related to its regulatory effect on the 
      activation of PI3 K/AKT signaling pathway and NF-kappa B signaling pathway. In 
      addition,emblin may be its effective anti-inflammation chemical composition.
FAU - Rao, Zhi-Li
AU  - Rao ZL
AD  - Pharmacy College of Chengdu University of Traditional Chinese Medicine Chengdu 
      611137,China.
FAU - Cao, Hai-Juan
AU  - Cao HJ
AD  - Pharmacy College of Chengdu University of Traditional Chinese Medicine Chengdu 
      611137,China.
FAU - Shi, Bo-Yu
AU  - Shi BY
AD  - Pharmacy College of Chengdu University of Traditional Chinese Medicine Chengdu 
      611137,China.
FAU - Luo, Jie
AU  - Luo J
AD  - Pharmacy College of Chengdu University of Traditional Chinese Medicine Chengdu 
      611137,China.
FAU - Liu, Xiao-Bo
AU  - Liu XB
AD  - Pharmacy College of Chengdu University of Traditional Chinese Medicine Chengdu 
      611137,China.
FAU - Zeng, Nan
AU  - Zeng N
AD  - Pharmacy College of Chengdu University of Traditional Chinese Medicine Chengdu 
      611137,China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Yao Za Zhi
JT  - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese 
      materia medica
JID - 8913656
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (interleukin-6, mouse)
RN  - 8PJ61P6TS3 (1-Butanol)
MH  - 1-Butanol
MH  - Animals
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - *Inflammation
MH  - Interleukin-1beta/metabolism
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides
MH  - Macrophages/*drug effects
MH  - Mice
MH  - Plant Extracts/*pharmacology
MH  - RAW 264.7 Cells
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Jingfang n-butanol extraction
OT  - LPS
OT  - NF-kappaB
OT  - PI3K/AKT
OT  - RAW264.7 cells
OT  - fractions A
EDAT- 2019/04/17 06:00
MHDA- 2019/08/15 06:00
CRDT- 2019/04/17 06:00
PHST- 2019/04/17 06:00 [entrez]
PHST- 2019/04/17 06:00 [pubmed]
PHST- 2019/08/15 06:00 [medline]
AID - 10.19540/j.cnki.cjcmm.20181214.004 [doi]
PST - ppublish
SO  - Zhongguo Zhong Yao Za Zhi. 2019 Mar;44(5):1026-1033. doi: 
      10.19540/j.cnki.cjcmm.20181214.004.