PMID- 30989886 OWN - NLM STAT- MEDLINE DCOM- 20190814 LR - 20190814 IS - 1001-5302 (Print) IS - 1001-5302 (Linking) VI - 44 IP - 4 DP - 2019 Feb TI - [Establishment of QAMS method with dehydroandrographolide as internal reference substance for quality control of Andrographis Herba]. PG - 730-739 LID - 10.19540/j.cnki.cjcmm.20181129.006 [doi] AB - To solve the problems of the poor resolution of chromatographic separation,the weak durability of the relative correction factors,and the low accuracy of content determination results in the quantitative analysis of multi-components by single-marker( QAMS) method with andrographolide as the internal reference substance in the existing research of Andrographis Herba,a new QAMS method using dehydroandrographolide as the internal reference substance was established for the first time in this study. This new method can be used to simultaneously determine four diterpene lactones,including andrographolide( A),neoandrographolide( B),14-deoxyandrographolide( C),and dehydroandrographolide( S) through the optimization of chromatographic conditions and systematic investigation of methodology. At the present HPLC chromatographic conditions,four components could be well separated( R > 1. 5),and the methodology validations could satisfy the requirement of quantitative analysis. The relative correction factors( RCFs) of fA/S,fB/S,fC/S were determined as 0. 65,0. 54,0. 78,respectively. The relative standard deviations( RSDs) of their RCFs ranged between 1. 3%-5. 1%,0. 25%-0. 33%,0. 070%-0. 15%,0. 070%-0. 22%,respectively with three brands of HPLC instruments,five brands of C18 column,different flow rates( 0. 9,1. 0,1. 1 m L.min~(-1)),and different column temperatures( 25,30,35 ℃),indicating good durability of the RCFs. The relative retention value( RRV) method was used to locate the chromatographic peak of the components to be determined.The RRVs of rA/S,rB/S,and rC/Swere 0. 44,0. 86,0. 97,respectively. The RSDs of the RRVs ranged between 0. 030%-1. 6% with different HPLC instruments and columns,showing accurate peak location. The present QAMS method and the external standard method( ESM)were both used to determine the contents of four diterpene lactones from Andrographis Herba( 6 batches of medicinal materials and 18 batches of cut crude drugs). The relative errors of the determined content results between two methods were less than 2. 0%. It demonstrated that there was no significant difference in content results between these two methods,indicating good accuracy of the present QAMS method. Therefore,in this study,an accurate and highly durable QAMS method using dehydroandrographolide as the internal reference substance was established for simultaneous determination of four diterpene lactones. This method could be used to effectively control the quality of Andrographis Herba and provide technical basis for the formulation of traditional Chinese medicine industry standard and improvement of the Chinese Pharmacopoeia standard of Andrographis Herba. FAU - Chen, Liang-Mian AU - Chen LM AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. FAU - Gao, Hui-Min AU - Gao HM AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. FAU - Hu, Xue-Hua AU - Hu XH AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China Yunnan University of Traditional Chinese Medicine Kunming 650500,China. FAU - Zhu, Jing-Jing AU - Zhu JJ AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. FAU - Feng, Wei-Hong AU - Feng WH AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. FAU - Yan, Li-Hua AU - Yan LH AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. FAU - Liu, Xiao-Qian AU - Liu XQ AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. FAU - Zhang, Yong-Xin AU - Zhang YX AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. FAU - Wang, Zhi-Min AU - Wang ZM AD - National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine,Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700,China. LA - chi PT - Journal Article PL - China TA - Zhongguo Zhong Yao Za Zhi JT - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JID - 8913656 RN - 0 (Diterpenes) RN - 0 (Drugs, Chinese Herbal) RN - TKB45D7LVX (dehydroandrographolide) MH - *Andrographis MH - Chromatography, High Pressure Liquid MH - Diterpenes MH - *Drugs, Chinese Herbal MH - Quality Control OTO - NOTNLM OT - Andrographis Herba OT - QAMS method OT - diterpene lactones OT - quality control of traditional Chinese medicine OT - relative correction factor EDAT- 2019/04/17 06:00 MHDA- 2019/08/15 06:00 CRDT- 2019/04/17 06:00 PHST- 2019/04/17 06:00 [entrez] PHST- 2019/04/17 06:00 [pubmed] PHST- 2019/08/15 06:00 [medline] AID - 10.19540/j.cnki.cjcmm.20181129.006 [doi] PST - ppublish SO - Zhongguo Zhong Yao Za Zhi. 2019 Feb;44(4):730-739. doi: 10.19540/j.cnki.cjcmm.20181129.006.