PMID- 30990028
OWN - NLM
STAT- MEDLINE
DCOM- 20200217
LR  - 20200217
IS  - 1520-5010 (Electronic)
IS  - 0893-228X (Linking)
VI  - 32
IP  - 5
DP  - 2019 May 20
TI  - Carbon Nanotube- and Asbestos-Induced DNA and RNA Methylation Changes in 
      Bronchial Epithelial Cells.
PG  - 850-860
LID - 10.1021/acs.chemrestox.8b00406 [doi]
AB  - Carbon nanotubes (CNTs) are nanoscale tube-shaped carbon materials used in many 
      industrial areas. Their fiber shape has caused concerns about their toxicity 
      given their structural similarity with asbestos. The aim here was to elucidate 
      the effect of CNTs and asbestos exposure on global DNA and RNA methylation and 
      the methylation of genes associated with cell cycle, inflammation, and DNA damage 
      processes in human lung cells. Human bronchial epithelial cells (16HBE14o-) were 
      exposed for 24 h to 25 and 100 mug/mL CNTs (single-walled CNTs [SWCNTs] and 
      multiwalled CNTs [MWCNTs]) and 2.5 mug/mL asbestos (chrysotile, amosite, and 
      crocidolite). Global DNA and RNA (hydroxy)methylation to cytosines was measured 
      by a validated liquid chromatography tandem-mass spectrometry method. Global RNA 
      methylation to adenines was measured by a colorimetric ELISA-like assay. 
      Gene-specific DNA methylation status at certain cytosine-phosphate-guanine (CpG) 
      sites of cyclin-dependent kinase inhibitor 1A ( CDKN1A), serine/threonine kinase 
      ( ATM), and TNF receptor-associated factor 2 ( TRAF2) were analyzed by using 
      bisulfite pyrosequencing technology. Only MWCNT-exposed cells showed significant 
      global DNA hypomethylation of cytosine and global RNA hypomethylation of 
      adenosine. SWCNT, MWCNT, and amosite exposure decreased DNA methylation of 
      CDKN1A. ATM methylation was affected by chrysotile, SWCNT, and MWCNT. However, 
      SWCNT exposure led to DNA hypermethylation of TRAF2. These findings contribute to 
      further understanding of the effect of CNTs on different carcinogenic pathways.
FAU - Emerce, Esra
AU  - Emerce E
AD  - Department of Public Health and Primary Care, Unit of Environment and Health , KU 
      Leuven , 3000 Leuven , Belgium.
AD  - Department of Toxicology, Faculty of Pharmacy , Gazi University , 06560 Ankara , 
      Turkey.
FAU - Ghosh, Manosij
AU  - Ghosh M
AD  - Department of Public Health and Primary Care, Unit of Environment and Health , KU 
      Leuven , 3000 Leuven , Belgium.
FAU - Oner, Deniz
AU  - Oner D
AD  - Department of Public Health and Primary Care, Unit of Environment and Health , KU 
      Leuven , 3000 Leuven , Belgium.
FAU - Duca, Radu-Corneliu
AU  - Duca RC
AD  - Department of Public Health and Primary Care, Unit of Environment and Health , KU 
      Leuven , 3000 Leuven , Belgium.
FAU - Vanoirbeek, Jeroen
AU  - Vanoirbeek J
AD  - Department of Public Health and Primary Care, Unit of Environment and Health , KU 
      Leuven , 3000 Leuven , Belgium.
FAU - Bekaert, Bram
AU  - Bekaert B
AD  - Forensic Biomedical Sciences, Department of Imaging and Pathology , KU Leuven - 
      University of Leuven , 3000 Leuven , Belgium.
AD  - Department of Forensic Medicine, Laboratory of Forensic Genetics and Molecular 
      Archaeology , University Hospitals Leuven , 3000 Leuven , Belgium.
FAU - Hoet, Peter H M
AU  - Hoet PHM
AD  - Department of Public Health and Primary Care, Unit of Environment and Health , KU 
      Leuven , 3000 Leuven , Belgium.
FAU - Godderis, Lode
AU  - Godderis L
AUID- ORCID: 0000-0003-4764-8835
AD  - Department of Public Health and Primary Care, Unit of Environment and Health , KU 
      Leuven , 3000 Leuven , Belgium.
AD  - External Service for Prevention and Protection at Work , IDEWE , B-3001 Leuven , 
      Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190423
PL  - United States
TA  - Chem Res Toxicol
JT  - Chemical research in toxicology
JID - 8807448
RN  - 0 (Nanotubes, Carbon)
RN  - 1332-21-4 (Asbestos)
RN  - 63231-63-0 (RNA)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Asbestos/*toxicity
MH  - Bronchi/cytology
MH  - Cell Cycle/drug effects
MH  - Cell Line
MH  - DNA/genetics/*metabolism
MH  - DNA Damage/drug effects
MH  - DNA Methylation/*drug effects
MH  - Epigenesis, Genetic/drug effects
MH  - Epithelial Cells/*drug effects
MH  - Humans
MH  - Inflammation/physiopathology
MH  - Nanotubes, Carbon/*toxicity
MH  - RNA/genetics/*metabolism
EDAT- 2019/04/17 06:00
MHDA- 2020/02/18 06:00
CRDT- 2019/04/17 06:00
PHST- 2019/04/17 06:00 [pubmed]
PHST- 2020/02/18 06:00 [medline]
PHST- 2019/04/17 06:00 [entrez]
AID - 10.1021/acs.chemrestox.8b00406 [doi]
PST - ppublish
SO  - Chem Res Toxicol. 2019 May 20;32(5):850-860. doi: 10.1021/acs.chemrestox.8b00406. 
      Epub 2019 Apr 23.